A novel method for spectrophotometric determination of pregabalin in pure form and in capsules

Abstract

BackgroundPregabalin, a γ-amino-n-butyric acid derivative, is an antiepileptic drug not yet official in any pharmacopeia and development of analytical procedures for this drug in bulk/formulation forms is a necessity. We herein, report a new, simple, extraction free, cost effective, sensitive and reproducible spectrophotometric method for the determination of the pregabalin.ResultsPregabalin, as a primary amine was reacted with ninhydrin in phosphate buffer pH 7.4 to form blue violet colored chromogen which could be measured spectrophotometrically at λmax 402.6 nm. The method was validated with respect to linearity, accuracy, precision and robustness. The method showed linearity in a wide concentration range of 50-1000 μg mL-1 with good correlation coefficient (0.992). The limits of assays detection was found to be 6.0 μg mL-1 and quantitation limit was 20.0 μg mL-1. The suggested method was applied to the determination of the drug in capsules. No interference could be observed from the additives in the capsules. The percentage recovery was found to be 100.43 ± 1.24.ConclusionThe developed method was successfully validated and applied to the determination of pregabalin in bulk and pharmaceutical formulations without any interference from common excipients. Hence, this method can be potentially useful for routine laboratory analysis of pregabalin.

Screening and optimization of γ-aminobutyric acid production fromMonascus sanguineusunder solid-state fermentation

“…Hosseinimehr et al (2010) reported a similar ninhydrin procedure for the estimation of baclofen, which is an analogue of GABA. The same protocol was applied by Bali and Gaur (2011) for the estimation of pregabalin, another analogue of GABA.…”

Mechanisms of L‐lysine extraction with sec‐octylphenoxy acetic acid in sulfonated kerosene

“…The extraction method of the competition experiment was in accordance with that detailed in the Extraction experiments section. The amino acids were analyzed by ninhydrin‐colorimetry method . A curve reflecting the influences of the three typical amino acids on L‐Lys extraction was plotted with the molar ratios of the other amino acids (L‐Arg, L‐Asp, L‐Ala) to L‐Lys as abscissa and the extraction efficiency as vertical coordinate, respectively.…”

Kinetic spectrophotometric determination of an important pharmaceutical compound, pregabalin

“…Several methods have been developed for the determination of the drug in pure and pharmaceutical preparations which are mostly based on chromatographic, spectrofluorimetric and spectrophotometric methods listed elsewhere (Bali and Gaur 2011). A detailed literature survey shows that few spectrophotometric methods have been developed for the determination of PGB in bulk and pharmaceutical preparation.…”