A Novel Method for Removal of Human Immunodeficiency Virus: Filtration with Porous Polymeric Membranes

Hamamoto, Yoshisuke; Harada, Shinji; Kobayashi, Susumu; Yamaguchi, Kazuhito; Iijima, Hideki; Manabe, Sei-ichi; Tsurumi, Takashi; Aizawa, Hiizu; Yamamoto, Naoki

doi:10.1111/j.1423-0410.1989.tb02034.x

Cited by 64 publications

(23 citation statements)

References 18 publications

(3 reference statements)

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“…Recently, large pore ultrafiltration membranes with MWCO (150$800 kDa) have been developed as virus filtration membranes (Christy and Vermant, 2002;Hamamoto et al, 1989;Maerz et al, 1996). Here the aim is to validate virus clearance by greatly reducing the number of contaminating viruses by retaining virions that may be present in the product stream.…”

Section: Introductionmentioning

confidence: 99%

Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification

Wickramasinghe

Kalbfuss

Zimmermann

et al. 2005

Biotechnol. Bioeng.

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Large scale purification of viruses and viral vectors for gene therapy applications and viral vaccines is a major separation challenge. Here tangential flow microfiltration and ultrafiltration using flat sheet membranes has been investigated for concentration of human influenza A virus. Ultrafiltration membranes with molecular weight cutoffs of 100 and 300 kDa as well as 0.1, 0.2 and 0.45 microm microfiltration membranes have been tested. The results indicate that use of 300 kDa membranes not only concentrate the virus particles but also lead to a significant removal of host cell proteins and DNA in the permeate. Tangential flow filtration may be used to fractionate virus particles. Human influenza A virus particles are spherical with an average size of 100 nm. Use of a 0.1 microm membrane leads to passage of virus particles less than 100 nm into the permeate and an increase of larger particles in the retentate. These results suggest that control of the transmembrane pressure, membrane pore size and pore size distribution could enable isolation of intact virus particles from damaged virions. Isolation of the virus particles of interest from viral fragments and other particulate matter could result in simplification of subsequent purification steps. Larger pore size membranes such as 0.45 microm that allow the passage of all virus particles may be used to remove host cell fragments. In addition virus particles attached to these fragments will be removed. Careful selection of membrane morphology and operating conditions will be essential in order to maximize the benefit of tangential flow filtration steps in the purification of viral products from cell cultures.

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Section: Introductionmentioning

confidence: 99%

Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification

Wickramasinghe

Kalbfuss

Zimmermann

et al. 2005

Biotechnol. Bioeng.

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“…We have validated a specific elimination treatment of vi ruses by filtration of two highly purified factor IX and fac tor XI concentrates [13,14] using newly developed cuprammonium-regenerated cellulose hollow fiber [15][16][17][18], Re sults show the efficiency of two membranes with mean pore sizes of 35 and 15 nm, respectively, at achieving steric re moval of enveloped and non-enveloped viruses of different sizes (from approximately 20 to 200 nm) and shapes. Pro tein recovery was good and no sign of alteration of the plas ma protein concentrates could be detected either in vitro or in animal models.…”

Section: Introductionmentioning

confidence: 99%

Nanofiltration, a New Specific Virus Elimination Method Applied to High-Purity Factor IX and Factor XI Concentrates

Burnouf-Radosevich¹,

Appourchaux²,

Huart³

et al. 1994

Vox Sanguinis

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We have validated the use of two new regenerated multilayered structured cellulose membranes (BMM), Planova 15 N and Planova 35 N, with respective mean pore sizes of 15 and 35 nm, as a new filtration system to eliminate viruses in highly purified factor IX and factor XI concentrates. Virus spiking experiments indicated that single dead-end filtration on the membranes could remove more than 5.7-7.8 log10 of human immunodeficiency virus, bovine viral diarrhoea virus, porcine pseudorabies virus, reovirus type 3, and simian virus 40, as well as the small non-enveloped viruses, poliovirus Sabin type 1 and bovine parvovirus. In vitro control tests and animal studies (Wessler stasis model, rat hypotension model) of the two concentrates did not reveal any significant differences with the non-nanofiltered material. Viral filtration of plasma derivatives on porous polymeric membranes might be an essential step in the improvement of their viral safety.

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“…A microporous membrane filter with an appropriate pore size is an effective tool for removal of HIV-1 particles from various aqueous samples [Hamamoto et al, 1989]. In fact, PLANOVA 75 TM (Asahi Chemical Industry Co., Tokyo, Japan), a commercially available virus removal filter with a mean pore size of 75 nm, achieved almost complete removal of viral particles from HIV-1 suspension (Table II).…”

Section: Resultsmentioning

confidence: 98%

Concanavalin A-immobilized polystyrene nanospheres capture HIV-1 virions and gp120: Potential approach towards prevention of viral transmission

et al. 1998

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To establish an effective tool for the prevention of HIV-1 transmission, lectin-immobilized polystyrene nanospheres were synthesized and examined for their HIV-1 capture activity. When concanavalin A (Con A) was immobilized on the surface of polystyrene nanospheres (400 nm in diameter) with poly(methacrylic acid) branches and incubated with HIV-1 suspension at room temperature for 60 min, the nanospheres (Con A-NSs) achieved a >3.3 log and a 2.2 log reduction of viral infectivity in HIV-1 (IIIB strain) suspension at a concentration of 2 and 0.5 mg/ml, respectively. Meanwhile, Con A-free nanospheres, which were not immobilized with Con A, achieved only a 0.29 log reduction at 0.5 mg/ ml. Con A-NSs (2 mg/ml) could also reduce the gp120 level of III(B) and HE strains to <7.1% and 5.5% of each control, respectively. The combination of Con A-NS treatment followed by filtration with a microporous membrane efficiently removed virion-free gp120 as well as infectious viral particles from HIV-1 suspension. Electron microscopic examination demonstrated that HIV-1 virions were trapped on the surface of Con A-NSs. Thus, Con A-NSs can capture HIV-1 virions and gp120 with high affinity, and may have potential as an effective tool for the prevention of HIV-1 transmission.

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A Novel Method for Removal of Human Immunodeficiency Virus: Filtration with Porous Polymeric Membranes

Cited by 64 publications

References 18 publications

Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification

Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification

Nanofiltration, a New Specific Virus Elimination Method Applied to High-Purity Factor IX and Factor XI Concentrates

Concanavalin A-immobilized polystyrene nanospheres capture HIV-1 virions and gp120: Potential approach towards prevention of viral transmission

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