A novel method for quantitative thin layer chromatography

Baum, D. E.; Shanks, Robert A.

doi:10.1021/ed052p738

J. Chem. Educ.

1975

DOI: 10.1021/ed052p738

|View full text |Cite

A novel method for quantitative thin layer chromatography

D. E. Baum¹,

Robert A. Shanks²

Abstract: The well-known paper chromatography method of quantitation by weighing cut out spot areas has been applied to thin layer chromatograms by a simple technique.Separation of phenol and 4,4'-diphenylolpropane was achieved by thin layer chromatography on a 0.2-mm layer of Silica Gel H'(Merck reagent) using two elutions with benzene-methanol-ethyl acetate (90:2:8)' for development. The

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2005

2008

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Modified Diatomaceous Earth as a Principal Stationary Phase Component in TLC

Ergül¹,

Kadan²,

Savaşcı³

et al. 2005

Journal of Chromatographic Science

View full text Add to dashboard Cite

Modified natural diatomaceous earth (DE) is a principal component of the stationary phase in normal thin-layer chromatography (TLC) applications and is mixed with commercial silica gel 60GF254 (Si-60GF254). Modification is carried out by flux calcination and refluxing with acid. Natural DE, modified DEs [flux calcinated (FC)DE and FCDE-I), and Si-60GF254 are characterized by scanning electron microscopy and Fourier-transform-IR spectroscopy. Particle size, specific surface area, pore distribution, pore volume, and surface hydroxyl group density parameters of materials are determined by various techniques. FCDE-I and Si-60GF254 are investigated for their usefulness in the stationary phase of TLC both individually and in composition. Commercially available red and blue ink samples are run on layers of Si-60GF254 and FCDE-I individually, and on various FCDE-I and Si-60GF254 mixtures. Butanol-ethanol-2M ammonia (3:1:1, v/v) and butanol-acetic acid-water (12:3:5, v/v) mixtures are used as mobile phases. The polarities of stationary phases decrease, and the retention factor (Rf) values of ink components increase when the FCDE-I content of the stationary phase increases. The properties of the stationary phase can be optimized by adding FCDE-I to Si-60GF254. This study may be useful in understanding both the systematic effects of stationary phase properties [e.g., specific surface area and surface hydroxyl group density, aOH(s)] and those of the mobile phase (e.g., polarity and acidity) on Rf values and the separability of components.

show abstract

Modified Diatomaceous Earth as a Principal Stationary Phase Component in TLC

Ergül¹,

Kadan²,

Savaşcı³

et al. 2005

Journal of Chromatographic Science

View full text Add to dashboard Cite

show abstract

Linkage Between Separation of Cu2+, Co2+, and Ni2+ on TLC and Crystal Field Theory

Ergül

2008

Journal of Chromatographic Science

View full text Add to dashboard Cite

M(DEDTC)2 (M = Cu, Co, or Ni) and M(PyDTC)2 (M = Cu or Co) complexes prepared by reactions of sodium diethyldithiocarbamate (NaDEDTC) and ammonium pyrrolidinedithiocarbamate (NH4PyDTC) with metal (II) nitrates are examined for qualitative analysis and separation using thin-layer chromatography (TLC) systems in the literature. Already known separability of metal cations cited and their complexes are reexamined and discussed in the context of relation to the crystal field theory (CFT) and TLC as a new approach and adaptation. Because the chromatographic data have been agreed on with assumptions of CFT, it is concluded that CFT is closely related to the TLC separation of these metal cations and their complexes. Therefore, this study may be useful in understanding of the linkage between the CFT on coordination chemistry and the chromatographic parameters [e.g., retention factor (Rf) and theoretical plate numbers (N)] of the complexes.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A novel method for quantitative thin layer chromatography

Cited by 2 publications

References 0 publications

Modified Diatomaceous Earth as a Principal Stationary Phase Component in TLC

Modified Diatomaceous Earth as a Principal Stationary Phase Component in TLC

Linkage Between Separation of Cu2+, Co2+, and Ni2+ on TLC and Crystal Field Theory

Contact Info

Product

Resources

About