A novel method for preparation of HAMLET-like protein complexes

Permyakov, Sergei E.; Knyazeva, Ekaterina L.; Leonteva, Marina V.; Фадеев, Р. С.; Chekanov, Aleksei V.; Zhadan, Andrei P.; Håkansson, Anders; Акатов, В. С.; Permyakov, Eugene A.

doi:10.1016/j.biochi.2011.05.002

Cited by 41 publications

(53 citation statements)

References 37 publications

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“…Furthermore, all the OA-bound states of -LA had a higher affinity for interaction with the membranes compared to -LA alone, indicating a Ca 2+ -independent association. Similar results were obtained regarding the bactericidal activity and membrane depolarization effect of HAMLET, bovine -lactoglobulin and pike parvalbumin in complex with OA on S. pneumococci bacteria [40,64]. Accordingly, it has been proposed that once the complex has adsorbed to the plasma membrane, it is most likely internalized by nonspecific pinocytosis, while other mechanisms of endocytosis, such as receptor-mediated endocytosis have not been ruled out [65].…”

Section: Cellular Trafficking Of Hamletsupporting

confidence: 70%

“…These include OA-conditioned anion exchange chromatography using either a DEAE-Trisacryl M in the conventional method [3,5,6,19] or a DEAE-Sepharose column in an alternative method [53]. Direct mixing under heat denaturation [30,36,52], heat denaturation of -LA and OA-conditioned anion exchange chromatography [62], direct mixing in solution at room temperature with alternated pH conditions [45,48,49,63], direct mixing followed by anion exchange chromatography [61] and alkaline conditions [64] have also been used.…”

Section: Methods Of Hamlet or Hamlet-analog Preparation In Vitromentioning

confidence: 99%

“…Regardless of the preparation method used in the different experiments, it has been shown that the cytotoxicity of the resulting complexes was similar to that of conventionally prepared HAMLET or BAMLET [30,36,48,49,54,[61][62][63][64][65].…”

Section: Methods Of Hamlet or Hamlet-analog Preparation In Vitromentioning

confidence: 99%

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The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

Jøhnke¹,

Petersen²

2012

Milk Protein

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Section: Cellular Trafficking Of Hamletsupporting

confidence: 70%

Section: Methods Of Hamlet or Hamlet-analog Preparation In Vitromentioning

confidence: 99%

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The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

Jøhnke¹,

Petersen²

2012

Milk Protein

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“…Together this indicates that the fatty acids play a crucial role in the cytotoxicity of HAMLET and BAMLET-like complexes. Furthermore, the comparability of the various complexes underlines that the cytotoxic complexes can be prepared using different methods as also shown by Permyakov [37].…”

Section: Discussionmentioning

confidence: 75%

The cytotoxicity of fatty acid/α‐lactalbumin complexes depends on the amount and type of fatty acid

Brinkmann

Brodkorb

Thiel

et al. 2013

Euro J Lipid Sci & Tech

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Complexes of the milk protein, α‐lactalbumin, and the fatty acid, oleic acid, have previously been shown to be cytotoxic. Complexes of α‐lactalbumin and five different fatty acids (vaccenic, linoleic, palmitoleic, stearic, and elaidic acid) were prepared and compared to those formed with oleic acid. All complexes were cytotoxic to human promyelocytic leukemia‐derived (HL‐60) cells but to different degrees depending on the fatty acid. The amount of fatty acid per α‐lactalbumin molecule was found to correlate with the cytotoxicity; the higher the number of fatty acids per protein, the more cytotoxic the complex. Importantly, all the tested fatty acids were also found to be cytotoxic on their own in a concentration dependent manner. The cytotoxic effect of complexes between α‐lactalbumin and linoleic acid, vaccenic acid, or oleic acid was further investigated using flow cytometry and found to induce cell death resembling apoptosis on Jurkat cells.Practical applications: Cytotoxic complexes of α‐lactalbumin and several different fatty acids could be produced. The cytotoxicity of all the variants is similar to that previously determined for α‐lactalbumin/oleic acid complexes.

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“…When complexed with oleic acid HAMLET exerts bactericidal effects against S. pneumoniae via calcium dependent membrane depolarization [70,71] . Furthermore, acetylation of cationic peptides has been shown to impart antimicrobial activity or increase their antimicrobial activity [72] .…”

Section: Synergism With Ampsmentioning

confidence: 99%

Antimicrobial lipids: Emerging effector molecules of innate host defense

Porter

Daniel²,

Alvarez³

et al. 2015

WJI

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The antimicrobial properties of host-derived derived lipids have become increasingly recognized and evidence is mounting that antimicrobial lipids (AMLs), like antimicrobial peptides, are effector molecules of the innate immune system and are regulated by its conserved pathways. This review, with primary focus on the human body, provides some background on the biochemistry of lipids, summarizes their biological functions, expands on their antimicrobial properties and site-specific composition, presents modes of synergism with antimicrobial peptides, and highlights the more recent reports on the regulation of AML production as well as bacterial resistance mechanisms. Based on extant data a concept of innate epithelial defense is proposed where epithelial cells, in response to microbial products and proinflammatory cytokines and through activation of conserved innate signaling pathways, increase their lipid uptake and up-regulate transcription of enzymes involved in antimicrobial lipid biosynthesis, and induce transcription of antimicrobial peptides as well as cytokines and chemokines. The subsequently secreted antimicrobial peptides and lipids then attack and eliminate the invader, assisted by or in synergism with other antimicrobial molecules delivered by other defense cells that have been recruited to the site of infection, in most of the cases. This review invites

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A novel method for preparation of HAMLET-like protein complexes

Cited by 41 publications

References 37 publications

The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

The cytotoxicity of fatty acid/α‐lactalbumin complexes depends on the amount and type of fatty acid

Antimicrobial lipids: Emerging effector molecules of innate host defense

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