A Novel Method for Predicting Disease-Associated LncRNA-MiRNA Pairs Based on the Higher-Order Orthogonal Iteration

Xuan, Zhanwei; Feng, Xin; Yu, Jingwen; Ping, Pengyao; Zhao, Haochen; Zhu, Xianyou; Wang, Lei

doi:10.1155/2019/7614850

Cited by 7 publications

(2 citation statements)

References 76 publications

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“…Long non-coding RNAs (lncRNAs) govern fundamental biochemical and cellular processes. To assess more important functional lncRNAs in non-small cell lung cancers (NSCLC), we examined the lncRNA-disease related database LncRNADisease v2.0 ( http://www.rnanut.net/lncrnadisease/index.php/home/search ), which was integrated comprehensive experimentally support and predicted ncRNA-disease associations curated from manual literatures and other resources [ 13 , 35 , 36 ], finding that a latent functional lncRNA, CBR3-AS1, is highly-expressed in NSCLC. To further validate the obtained results in database, we detected the expression of lncRNA CBR3-AS1 in three subtypes of NSCLC, 60 LAD, 40 LCC and 40 LSCC patient tumor tissues compared with its normal specimens.…”

Section: Resultsmentioning

confidence: 99%

LncRNA CBR3-AS1 potentiates Wnt/β-catenin signaling to regulate lung adenocarcinoma cells proliferation, migration and invasion

Hou

Yao

2021

Cancer Cell Int

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Background Long non-coding RNAs (lncRNAs) are pervasively transcribed in genome and emerging as a new player in tumorigenesis due to their functions in transcriptional, posttranscriptional and epigenetic mechanisms of gene regulation. As the most frequent malignancy and the foremost source of cancer mortality, lung cancer is a heterogeneous disorder. The most common type of lung cancer is Non-small cell lung cancer (NSCLC), occupying 85% of the total cases, and the main subtypes of NSCLC include lung adenocarcinoma (LAD), large cell carcinoma (LCC), and lung squamous cell carcinoma (LSCC). Recently, numerous lncRNAs have been reported to be strongly linked to NSCLC. In the present study, we found that a new lncRNA CBR3-AS1 is highly expressed in lung cancer. In addition, we also examined the expression of lncRNA CBR3-AS1 in 60 of LADs, 40 of LCCs and 40 of LSCCs patient samples, finding that CBR3-AS1 was specificity highly expressed in LAD cancer tissues. Mechanically, we discovered that CBR3-AS1 could regulate the proliferation, migration and invasion of LAD cells through targeting Wnt/β-catenin signaling. Methods Real-time PCR, RNA-pulldown, RIP, western blotting, lentivirus transfection, luciferase reporter assays, cell proliferation assays, colony formation assays, wound healing scratch assays and transwell assays were employed to examine the relationship between lncRNA CBR3-AS1 and its regulation of Wnt/β-catenin signaling in LAD cells. Results LncRNA CBR3-AS1 is highly-expressed in LAD and cell lines. LncRNA CBR3-AS1 shows physical association with β-catenin. CBR3-AS1 could facilitate Wnt/β-catenin signaling activation thought promoting nuclear localization of β-catenin. CBR3-AS1 promotes LAD cell proliferation, migration and invasion by targeting Wnt/β-catenin signaling. Conclusion It can be found that a new functional lncRNA CBR3-AS1 could promote nuclear localization of β-catenin so as to facilitate Wnt/β-catenin signaling activation and regulate the proliferation, migration and invasion of LAD cells.

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Section: Resultsmentioning

confidence: 99%

LncRNA CBR3-AS1 potentiates Wnt/β-catenin signaling to regulate lung adenocarcinoma cells proliferation, migration and invasion

Hou

Yao

2021

Cancer Cell Int

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“…It can degrade mRNA, inhibit translation and protein synthesis, and regulate genes by binding to the 3’-untranslated regions (3’-UTR) of mRNA. 4 , 5 MiRNA has been proved to play an important role in biological growth and development of organisms, metabolism and the occurrence and development of diseases. 6 Studies have shown that miRNA can be released from cells and participate in inflammation and tissue damage.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and Prognostic Value of Dysregulated miR-10a-3p in Patients with Severe Pneumonia

Xie¹,

Li²,

Wang³

et al. 2022

JIR

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Previous studies have shown that microRNA is involved in regulating a variety of human inflammatory diseases. The purpose of this study was to investigate the expression of miR-10a-3p in the blood of patients with severe pneumonia and evaluate its value in the diagnosis and prognosis of severe pneumonia. Patients and Methods: Seventy patients with severe pneumonia and 75 healthy individuals were included in this study. Venous blood of all subjects was obtained for RT-qPCR analysis to obtain the relative expression level of miR-10a-5p. The diagnostic accuracy of miR-10a-5p for severe pneumonia was assessed by ROC curve. After standardized treatment, the prognosis of patients with severe pneumonia was analyzed by a 28-day follow-up method. Kaplan-Meier curve and multivariate Cox regression analysis were used to determine the basic factors influencing the prognosis of patients. Results: Compared with healthy control, serum miR-10a-3p expression in patients with severe pneumonia was distinctly upregulated (P < 0.001). Besides, ROC analysis showed that miR-10a-3p had high diagnostic accuracy for severe pneumonia, with an AUC of 0.881, sensitivity and specificity of 75.7% and 84.0%, respectively. Kaplan-Meier curve exhibited that high miR-10a-3p expression group had a higher probability of death than those with low miR-10a-3p expression. Multivariate Cox regression analysis demonstrated that miR-10a-3p and CRP were independent risk factors affecting the prognosis of patients. Conclusion:The expression of miR-10a-3p was increased in patients with severe pneumonia, and abnormally expressed miR-10a-3p has the potential to be used as a diagnostic and prognostic marker for severe pneumonia, which provides a new biological direction for the early detection and risk assessment of severe pneumonia.

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Bioinformatics Approaches for Functional Prediction of Long Noncoding RNAs

Seifuddin

Pirooznia

2021

Methods in Molecular Biology

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A Novel Method for Predicting Disease-Associated LncRNA-MiRNA Pairs Based on the Higher-Order Orthogonal Iteration

Cited by 7 publications

References 76 publications

LncRNA CBR3-AS1 potentiates Wnt/β-catenin signaling to regulate lung adenocarcinoma cells proliferation, migration and invasion

LncRNA CBR3-AS1 potentiates Wnt/β-catenin signaling to regulate lung adenocarcinoma cells proliferation, migration and invasion

Diagnostic and Prognostic Value of Dysregulated miR-10a-3p in Patients with Severe Pneumonia

Bioinformatics Approaches for Functional Prediction of Long Noncoding RNAs

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