A novel method for multiplex protein biomarker analysis of human serum using quantitative MALDI mass spectrometry

Taraskin, Aleksandr S.; Semenov, Konstantin K.; Lozhkov, Alexey A.; Baranovskaya, Irina; Protasov, A. V.; Ramsay, Edward; Tyulin, Alexandr A.; Mirgorodskaya, O. A.; Васин, А. В.; Klotchenko, Sergey A.; Zabrodskaya, Yana A.

doi:10.1016/j.jpba.2021.114575

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The level of IL-6 and IL-10 is especially increased in critical patients [ 25 , 29 ]. In previous work [ 30 ], we have also discovered increased serum level of IL-10, IL-6, as well as acute-phase proteins (CRP and Serum amyloid A1) in the same patients with SARS-CoV-2 induced viral pneumonia. This indicates that the investigated group of patients with SARS-CoV-2 infection suffered from exuberant systematic inflammation.…”

Section: Discussionmentioning

confidence: 57%

Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response

Lozhkov

Plotnikova

Егорова

et al. 2022

Viruses

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In this study, we developed a novel, multiplex qPCR assay for simultaneous detection of RIG-1, MDA5, and IFIT-1 at the mRNA level. The assay was validated in A549 cells transfected with in vitro transcribed RNAs. Both exogenous RNA-GFP and self-amplifying (saRNA-GFP) induced significant expression of RIG-1, MDA5, IFIT-1, as well as type I and III interferons. In contrast, native RNA from intact A549 cells did not upregulate expression of these genes. Next, we evaluated RIG-1, MDA5, and IFIT-1 mRNA levels in the white blood cells of patients with influenza A virus (H3N2) or SARS-CoV-2. In acute phase (about 4 days after disease onset) both viruses induced these genes expression. Clinical observations of SARS-CoV-2 typically describe a two-step disease progression, starting with a mild-to-moderate presentation followed by a secondary respiratory worsening 9 to 12 days after the first onset of symptoms. It revealed that the expression of RIG-1, MDA5, and MxA was not increased after 2 and 3 weeks from the onset the disease, while for IFIT-1 it was observed the second peak at 21 day post infection. It is well known that RIG-1, MDA5, and IFIT-1 expression is induced by the action of interferons. Due to the ability of SOCS-1 to inhibit interferon-dependent signaling, and the distinct antagonism of SARS-CoV-2 in relation to interferon-stimulated genes expression, we assessed SOCS-1 mRNA levels in white blood cells. SARS-CoV-2 patients had increased SOCS-1 expression, while the influenza-infected group did not differ from heathy donors. Moreover, SOCS-1 mRNA expression remained stably elevated during the course of the disease. It can be assumed that augmented SOCS-1 expression is one of multiple mechanisms that allow SARS-CoV-2 to escape from the interferon-mediated immune response. Our results implicate SOCS-1 involvement in the pathogenesis of SARS-CoV-2.

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Section: Discussionmentioning

confidence: 57%

Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response

Lozhkov

Plotnikova

Егорова

et al. 2022

Viruses

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“…Furthermore, multiple studies identified low serum fetuin-A associated with of disease severity, hospitalization duration, and mortality of COVID-19 patients [ 27 , 60 , 122 ]. Accordingly, the lowest levels of circulating fetuin-A were found in critically ill sepsis patients [ 54 , 115 ]. Along these lines, systemic fetuin-A levels reflect acute inflammatory disease states as well as calcification propensity.…”

Section: Fetuin-a and Inflammationmentioning

confidence: 99%

Tissue chaperoning—the expanded functions of fetuin-A beyond inhibition of systemic calcification

Rudloff

Jahnen-Dechent

Huynh‐Do

2022

Pflugers Arch - Eur J Physiol

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Traditionally, fetuin-A embodies the prototype anti-calcification protein in the blood, preventing cardiovascular calcification. Low serum fetuin-A is generally associated with mineralization dysbalance and enhanced mortality in end stage renal disease. Recent evidence indicates that fetuin-A is a crucial factor moderating tissue inflammation and fibrosis, as well as a systemic indicator of acute inflammatory disease. Here, the expanded function of fetuin-A is discussed in the context of mineralization and inflammation biology. Unbalanced depletion of fetuin-A in this context may be the critical event, triggering a vicious cycle of progressive calcification, inflammation, and tissue injury. Hence, we designate fetuin-A as tissue chaperone and propose the potential use of exogenous fetuin-A as prophylactic agent or emergency treatment in conditions that are associated with acute depletion of endogenous protein.

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A novel method for multiplex protein biomarker analysis of human serum using quantitative MALDI mass spectrometry

Cited by 2 publications

References 25 publications

Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response

Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response

Tissue chaperoning—the expanded functions of fetuin-A beyond inhibition of systemic calcification

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