A novel method for human oocyte vitrification with a closed device using super-cooled air

Machac, S; Hubinka, V.; Larman, Mark G.; Koudelka, M

doi:10.1016/j.fertnstert.2013.07.1686

Cited by 2 publications

(1 citation statement)

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“…Following ICSI, the fertilization rate was 76 %. Blastocyst transfer resulted in a 49 % ongoing pregnancy rate, with five healthy live births reported so far [98].…”

Section: Closed Systemsmentioning

confidence: 99%

Cryopreservation in ART and concerns with contamination during cryobanking

Larman¹,

Hashimoto

Morimoto

2014

Reprod Medicine & Biology

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The cryopreservation of gametes and embryos is vital to numerous fields of reproductive biology, including assisted human reproduction. With improved culture conditions, there are an increasing number of embryos to cryopreserve for potential use in subsequent cycles. Many of the gametes and embryos in human IVF are cryopreserved in open systems. Because liquid nitrogen is not sterile, concerns have been raised with regard to contamination from the liquid nitrogen and also cross-contamination between patients' germplasm. Human gamete and embryo cryopreservation are discussed, with recommendations on how to minimize and eliminate contamination, emphasizing the benefits of closed vitrification devices.

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“…Following ICSI, the fertilization rate was 76 %. Blastocyst transfer resulted in a 49 % ongoing pregnancy rate, with five healthy live births reported so far [98].…”

Section: Closed Systemsmentioning

confidence: 99%

Cryopreservation in ART and concerns with contamination during cryobanking

Larman¹,

Hashimoto

Morimoto

2014

Reprod Medicine & Biology

View full text Add to dashboard Cite

show abstract

Appendix E: Rapid-iTM: Closed Vitrification Device by Vitrolife

Larman

2017

Methods in Molecular Biology

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Cryopreservation of gametes and embryos is a growing technique in numerous reproductive fields including human-assisted reproduction. With improved understanding of embryo physiology and optimized culture conditions, there are now more embryos than ever to vitrify for potential use in subsequent cycles. Many gametes and embryos have been cryopreserved in open systems, but there are concerns with regard to contamination from the liquid nitrogen and also cross-contamination between patients' germplasm. The development of the Rapid-i™, a closed vitrification device that does not use direct contact with liquid nitrogen during vitrification or subsequent storage, will be discussed as well as clinical protocols for human oocytes and embryos.

show abstract

A novel method for human oocyte vitrification with a closed device using super-cooled air

Cited by 2 publications

References 0 publications

Cryopreservation in ART and concerns with contamination during cryobanking

Cryopreservation in ART and concerns with contamination during cryobanking

Appendix E: Rapid-iTM: Closed Vitrification Device by Vitrolife

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