A novel method for encapsulation of macromolecules in liposomes

Shew, Ronald L.; Deamer, David W.

doi:10.1016/0005-2736(85)90386-4

Cited by 116 publications

(54 citation statements)

References 12 publications

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“…Furthermore, under these conditions amphiphilic compounds present as micelles or vesicles assemble into multilamellar structures on the surface, and any solutes that are present are concentrated and organized between lamellae (Toppozini et al, 2013). A subsequent rehydration cycle produces large lipid vesicles that have encapsulated a significant fraction of the solutes (Shew and Deamer, 1985). This observation gave rise to the hypothesis that chemical reactions could be promoted in concentrated films.…”

Section: Hydrothermal Fieldsmentioning

confidence: 94%

Lipids as Universal Biomarkers of Extraterrestrial Life

Georgiou

Deamer

2014

Astrobiology

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The conditions and properties of hydrothermal vents and hydrothermal fields are compared in terms of their ability to support processes related to the origin of life. The two sites can be considered as alternative hypotheses, and from this comparison we propose a series of experimental tests to distinguish between them, focusing on those that involve concentration of solutes, self-assembly of membranous compartments and synthesis of polymers.

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Section: Hydrothermal Fieldsmentioning

confidence: 94%

Lipids as Universal Biomarkers of Extraterrestrial Life

Georgiou

Deamer

2014

Astrobiology

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“…For encapsulation efficiency measurement (Shew & Deamer, 1985), a 0.1 ml liposome suspension was passed through a Sephadex G-50 gel-filtration column, and the contents of PTX and DOX were measured by high-performance liquid chromatography (Agilent Technologies Inc., Santa Clara, CA) through HPLC method at 227 nm and a fluorospectrophotometry (Ex ¼ 470 nm, Em ¼ 590 nm), respectively. The encapsulation efficiency was calculated as follows:…”

Section: Characterization Of Drug Loaded Liposomesmentioning

confidence: 99%

Targeted delivery of transferrin and TAT co-modified liposomes encapsulating both paclitaxel and doxorubicin for melanoma

et al. 2015

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The purpose of this study was to develop an efficient dual-ligand based liposomal drug delivery system with targeting specificity as well as properties that would kill melanoma cells. Liposomes modified with transferrin (Tf) and cell-penetrating peptide TAT was prepared, which encapsulated two kinds of chemotherapy drugs, paclitaxel and doxorubicin (Tf/TAT-PTX/DOX-LP). The Tf ligands specifically bind to the overexpressed Tf receptors on the surface of melanoma cells, while the TAT ligands functioned as a classical cell penetrating peptide, helping dual-ligand liposomes be internalized by melanoma cells. The effect of dual-targeting system and ''double-drug'' combination therapy were evaluated both in vitro and in vivo. In vitro, cellular uptake, intracellular distribution and tumor spheroids penetration studies demonstrated that the system could not only be selectively and efficiently penetrate melanoma cells. Besides, apoptosis staining assay and cytotoxicity showed effective anti-tumor capability and obvious synergistic effect of combination therapy of PTX and DOX. In vivo imaging and fluorescent images of tumor section further demonstrated that Tf/TAT-PTX/DOX-LP had the highest tumor distribution. The results of these experiments demonstrated that double-drug liposomal drug delivery systems (DDS) had both enhanced targeting efficiency and increased therapeutic efficacy.

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“…Another method (16) that produces dehydration-rehydration vesicles (DRV) is both simple and easy to scale up, and usually gives high yields of solute entrapment (up to 80%) (16)(17)(18)(19)(20). Another advantage of the DRV method compared to the REV method is that it does not expose the solute to potentially denaturating organic solvents and/or to sonication.…”

Section: Methods For Liposome Preparation and Solute Encapsulationmentioning

confidence: 99%

Liposomes: from biophysics to the design of peptide vaccines

Frézard

1999

Braz J Med Biol Res

111

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Liposomes (lipid-based vesicles) have been widely studied as drug delivery systems due to their relative safety, their structural versatility concerning size, composition and bilayer fluidity, and their ability to incorporate almost any molecule regardless of its structure. Liposomes are successful in inducing potent in vivo immunity to incorporated antigens and are now being employed in numerous immunization procedures. This is a brief overview of the structural, biophysical and pharmacological properties of liposomes and of the current strategies in the design of liposomes as vaccine delivery systems.

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A novel method for encapsulation of macromolecules in liposomes

Cited by 116 publications

References 12 publications

Lipids as Universal Biomarkers of Extraterrestrial Life

Lipids as Universal Biomarkers of Extraterrestrial Life

Targeted delivery of transferrin and TAT co-modified liposomes encapsulating both paclitaxel and doxorubicin for melanoma

Liposomes: from biophysics to the design of peptide vaccines

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