A novel method for distinguishing between dsDNA and ssRNA virus infections

Nuutila, Jari; Hohenthal, Ulla; Laitinen, Iina; Kotilainen, Pirkko; Rajamäki, Allan; Nikoskelainen, J; Lilius, Esa‐Matti

doi:10.1016/j.jcv.2008.04.004

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…Neutrophil CD64 has been used as a marker for sepsis [32-35] and to distinguish between infections with dsDNA and ssRNA viruses [35,36]. It has been suggested that neutrophil CD64 expression can be used to aid in the diagnosis of infections in patients with rheumatoid arthritis [37].…”

Section: Discussionmentioning

confidence: 99%

Monocyte surface expression of Fcγ receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus

Lee

Kellner

et al. 2010

Arthritis Res Ther

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IntroductionMore than half of systemic lupus erythematosus (SLE) patients show evidence of excess type I interferon (IFN-I) production, a phenotype associated with renal disease and certain autoantibodies. However, detection of IFN-I proteins in serum is unreliable, and the measurement of interferon-stimulated gene (ISG) expression is expensive and time consuming. The aim of this study was to identify a surrogate marker for IFN-I activity in clinical samples for monitoring disease activity and response to therapy.MethodsMonocyte surface expression of Fcγ receptors (FcγRs), chemokine receptors, and activation markers were analyzed with flow cytometry in whole blood from patients with SLE and healthy controls. FcγR expression also was measured in peripheral blood mononuclear cells (PBMCs) from healthy controls cultured with Toll-like receptor (TLR) agonists, cytokines, or serum from SLE patients. Expression of ISGs was analyzed with real-time PCR.ResultsCirculating CD14+ monocytes from SLE patients showed increased surface expression of FcγRI (CD64). The mean fluorescent intensity of CD64 staining correlated highly with the ISG expression (MX1, IFI44, and Ly6E). In vitro, IFN-I as well as TLR7 and TLR9 agonists, induced CD64 expression on monocytes from healthy controls. Exposure of monocytes from healthy controls to SLE sera also upregulated the expression of CD64 in an IFN-I-dependent manner. Decreased CD64 expression was observed concomitant with the reduction of ISG expression after high-dose corticosteroid therapy.ConclusionsExpression of CD64 on circulating monocytes is IFN-I inducible and highly correlated with ISG expression. Flow-cytometry analysis of CD64 expression on circulating monocytes is a convenient and rapid approach for estimating IFN-I levels in SLE patients.

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Section: Discussionmentioning

confidence: 99%

Monocyte surface expression of Fcγ receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus

Lee

Kellner

et al. 2010

Arthritis Res Ther

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“…Some of these tools include the analysis of neutrophil activation markers, such as CD64 (FcgRI) and CD35 (also known as complement receptor 1, CR1), which are expressed at different levels in patients infected with bacteria or viruses ( Jalava-Karvinen et al, 2009). This method has been used to distinguish between bacteria-and virusinduced arthritis (Mokuda et al, 2012), and between dsDNA and ssRNA virus infections (Nuutila et al, 2008). Moreover, bacterial and viral infections could also be distinguished by a lumino-dependent chemiluminescence assay that measures the amount of ROS produced by neutrophils upon activation (Prilutsky et al, 2011).…”

Section: Neutrophils As a Tool To Distinguish Between Bacterial And Vmentioning

confidence: 99%

Neutrophils: Neglected Players in Viral Diseases

Gabriel

Her

2013

DNA and Cell Biology

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“…In all the other cases, CF and HF were 1. At a cutoff point of higher than or equal to 1.5, the DNAVS points differentiated between dsDNA and ssRNA virus infections with 95% sensitivity and 100% specificity [11]. …”

Section: Distinguishing Between Dsdna and Ssrna Virus Infections Wmentioning

confidence: 99%

Bacterial Infections, DNA Virus Infections, and RNA Virus Infections Manifest Differently in Neutrophil Receptor Expression

Lilius

Nuutila

2012

The Scientific World Journal

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Treating viral illnesses or noninfective causes of inflammation with antibiotics is ineffective and contributes to the development of antibiotic resistance, toxicity, and allergic reactions, leading to increasing medical costs. A major factor behind unnecessary use of antibiotics is, of course, incorrect diagnosis. For this reason, timely and accurate information on whether the infection is bacterial in origin would be highly beneficial. In this paper we will present our recent studies on the expression of opsonin receptors on phagocytes. The analysis of the expression levels of FcγRI, CR1, and CR3, along with CRP and ESR data, provides a novel application to the diagnosis of infectious and inflammatory diseases. The best clinical benefit will be obtained when the individual variables are combined to generate the CIS point method for a bacterial infection marker, DNAVS point for differentiating between DNA and RNA virus infections, and CRP/CD11b ratio for a marker of Gram-positive sepsis.

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A novel method for distinguishing between dsDNA and ssRNA virus infections

Cited by 11 publications

References 16 publications

Monocyte surface expression of Fcγ receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus

Monocyte surface expression of Fcγ receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus

Neutrophils: Neglected Players in Viral Diseases

Bacterial Infections, DNA Virus Infections, and RNA Virus Infections Manifest Differently in Neutrophil Receptor Expression

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