A novel method for differentiating L-form bacteria from their parental form using the Hucker Gram staining technique

Allan, Eunice J.; Jaß, Jana; Phillips, Lisa E.; Costerton, J. W.; Lappin‐Scott, Hilary M.

doi:10.1111/j.1472-765x.1992.tb00761.x

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…In our work, the maximum culture absorbance of E. faecium L-forms did not correspond to that of the parent; we attribute this to the absence of the cell wall, the large transparent bodies formed by the L-form cells and the presence of vacuoles. Throughout the growth of the L-forms, neither full nor partial reversions to the parent form occurred as demonstrated on either agar plates or when Gram stained [15] and viewed using light microscopy. Whether this type of L-form stability is present in vivo is not known.…”

Section: Resultsmentioning

confidence: 91%

“…During the colonisation studies, samples of the L-forms were regularly removed from the culture reservoir and checked for reversion to the cell walled state using a modified Gram stain [15]. The silastic surfaces were prepared for scanning electron microscopy by fixing in a solution consisting of 3% glutaraldehyde in cacodylate buffer (0.1 M, pH 7.4) for 2 h at room temperature or overnight at 4°C, followed by dehydration in a series of aqueous-ethanol solutions (30, 50, 70 and 100%) and then air dried.…”

Section: Methodsmentioning

confidence: 99%

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Growth and adhesion ofEnterococcus faeciumL-forms

Jaß

Phillips

Allan

et al. 1994

FEMS Microbiology Letters

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Comparisons of growth and surface colonisation of Enterococcus faecium L-forms and their cell-walled forms were undertaken to produce information about their ability to form sessile cells. The growth of L-forms in liquid culture was slower than that of the parent. This was reflected in their longer lag phase and slower specific growth rates: 0.16 h-1 for the L-form and 0.81 h-1 for the parent. Although E. faecium L-forms attached to a silastic rubber surface, the attached population density was 10-100-fold less than that of the parent. Confluent biofilms on the silastic surfaces were not observed for either bacterial form. Comparison of the attachment of E. faecium L-form and parent may provide important information on how bacteria overcome host defence mechanisms and antibiotic treatment.

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Section: Resultsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Growth and adhesion ofEnterococcus faeciumL-forms

Jaß

Phillips

Allan

et al. 1994

FEMS Microbiology Letters

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“…Detection of bacterial L-forms traditionally involves weeks of incubation in a selective medium, with ambigious results. Allan et al (1992) used a modification of the gram stain to differentiate L-forms of Enterococcusfaecium, Bacillus subtilis and Pseudomonas syringae from their parental forms. The use of in vivo bioluminescence for detecting and studying foodborne organisms is gaining acceptance (Baker et al, 1992;Stewart and Williams, 1992).…”

Section: Introductionmentioning

confidence: 99%

Infection and removal of L-forms of Listeria monocytogenes with bred bacteriophage

Hibma

Jassim

Griffiths

1997

International Journal of Food Microbiology

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Phage breeding was employed to produce a bacteriophage (Listeria monocytogenes phage ATCC 23074-B1) which was specific for L-forms of L. monocytogenes. The bred phage was compared to its unbred parent for lytic activity and specificity. It was also tested for its ability to prevent L-form biofilm formation on stainless steel and compared with an organic acid (lactic) at L-form biofilm inactivation on stainless steel. The bred phage lysed only L-forms of L. monocytogenes in broth culture and only plaqued on L-form lawns. Likewise, the unbred phage performed similarly with classical cell-walled culture and lawns. The bred phage successfully inhibited L-form biofilm formation on stainless steel and was as successful as lactic acid (130 ppm) at inactivating pre-formed L-form biofilms. Both reduced viable cell numbers by 3-long cycles over a 6 h period. It appears that phage breeding technology may be an attractive alternative to chemical sanitizers which lack specificity and can be toxic.

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The potential of L‐form bacteria in biotechnology

Grichko

Glick

1999

Can J Chem Eng

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L-form bacteria include both stable and unstable cell wall-less variants of native walled bacteria. These organisms have been implicated as etiologic agents in both plant and animal diseases. L-form bacteria may also be used to protect plants or animals against disease-causing organisms, or to act as a host bacterium for the production and secretion to the growth medium of recombinant proteins. This article summarizes the fundamental biology of these unusual bacteria and discusses their potential uses.Les bacteries en forme de L englobent des variantes sans parois de cellules stables et instables de bacteries avec parois originales. Ces organismes ont ete impliques comme agents etiologiques dans les maladies vegetales et animales. Les bacteries en forme de L peuvent egalement servir a proteger les plantes ou les animaux contre des organismes causant des maladies, ou a titre de bacteries hBtes pour la production et la secretion de milieu de croissance de proteines recombinantes. On resume dans cet article la biologie fondamentale de ces bacteries inhabituelles et on analyse leurs utilisations potentielles.

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A novel method for differentiating L-form bacteria from their parental form using the Hucker Gram staining technique

Cited by 7 publications

References 9 publications

Growth and adhesion ofEnterococcus faeciumL-forms

Growth and adhesion ofEnterococcus faeciumL-forms

Infection and removal of L-forms of Listeria monocytogenes with bred bacteriophage

The potential of L‐form bacteria in biotechnology

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