A novel method for dengue virus detection and antibody screening using a graphene-polymer based electrochemical biosensor

Navakul, Krongkaew; Warakulwit, Chompunuch; Yenchitsomanus, Pa‐thai; Panya, Aussara; Lieberzeit, Peter A.; Sangma, Chak

doi:10.1016/j.nano.2016.08.009

Cited by 112 publications

(56 citation statements)

References 36 publications

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“…albumin, lysozyme [23] and nuclear matrix protein 22 (NMP22) [24]) and even microorganisms (e.g. viruses [25], bacteria [26] and algae [27]) have been used as the imprinting templates. Moreover, molecularly imprinted polymers can be integrated on microfluidic systems for either dielectric or electrochemical sensing of viruses [28] and urinary melatonin [29], respectively.…”

Section: Introductionmentioning

confidence: 99%

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

et al. 2017

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Three peptides (each containing 13-18 amino acids) were synthesized and used as templates for molecular imprinting and epitope recognition of the regenerating Protein 1B (REG1B), which is one of the urinary biomarkers for pancreatic ductal adenocarcinoma (PDAC). Poly(ethylene-co-vinyl alcohol)s were employed as the host for molecular imprinting of the peptides. Following their preparation, the molecularly imprinted polymers (MIP) were examined by cyclic voltammetry. The electrochemical responses of a screen-printed gold substrate coated with the MIP were measured at a working voltage of 300 mV (vs. Ag/AgCl); the entire protein and the peptides gave similar responses at concentrations of <1.0 pg⋅mL⁻¹, with detection limits as low as 0.1 pg⋅mL⁻¹. Urine samples from healthy and PDAC patients were then analyzed by using this modified gold electrode, and the results are in agreement with data obtained with ELISA.

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Section: Introductionmentioning

confidence: 99%

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

et al. 2017

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“…In particular, Dickert and Lieberzelt developed a plethora of platforms (principally based on quartz microbalance) with molecularly imprinted polymers (MIPs) to detect cells [47], bacteria [48], and viruses [49]. In addition, Lieberzelt and his group recently reported some electrochemical applications with MIPs: in 2014 [50], Cu 2+ ions were conductometrically detected down to 0.2 mM by polymerizing N-vinyl-2-pyrrolidone and, in 2016 [51], dengue virus was impedimetrically detected with a 0.12 pfu/mL detection limit by using a graphene oxide-polymer composite. Even the group of Cleij in Maastrischt, which is one of the most active regarding the use of MIPs, developed an impedimetric assay to detect histamine in intestinal fluid patient samples [52].…”

Section: Polymers As the Sensing Elementmentioning

confidence: 99%

Polymeric Materials for Printed-Based Electroanalytical (Bio)Applications

Cinti

2017

Chemosensors

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Advances in design of selective interfaces and printed technology have mighty contributed to the expansion of the electroanalysis fame. The real advantage in electroanalytical field is the possibility to manufacture and customize plenty of different sensing platforms, thus avoiding expensive equipment, hiring skilled personnel, and expending economic effort. Growing developments in polymer science have led to further improvements in electroanalytical methods such as sensitivity, selectivity, reproducibility, and accuracy. This review provides an overview of the technical procedures that are used in order to establish polymer effectiveness in printed-based electroanalytical methods. Particular emphasis is placed on the development of electronalytical sensors and biosensors, which highlights the diverse role of the polymeric materials depending on their specific application. A wide overview is provided, taking into account the most significant findings that have been reported from 2010 to 2017.

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“…The composites were prepared by using a previously reported method: acrylamide (AAM), methyl methacrylate (MMA), methacrylic acid (MAA), and n‐vinylpyrrolidone (NVP) were mixed with graphene oxide sheets. In the composites, graphene oxide was used to enhance the polymer conductivity, making it suitable for EIS measurements . The monomers served as sensitive layer building blocks with side‐chain groups covering a range of interactions, which were present on the virus surface and BPA.…”

Section: Figurementioning

confidence: 99%

“…The composites were prepared by using ap reviously reported method: [48] acrylamide (AAM), methyl methacrylate (MMA), methacrylic acid (MAA), and n-vinylpyrrolidone (NVP) were mixed with graphene oxide sheets. In the composites, graphene oxide was used to enhance the polymer conductivity,m aking it suitable for EIS measurements.…”

Section: Mcipsa Nd Mips Eis Gold Electrode Preparationsmentioning

confidence: 99%

“…In the composites, graphene oxide was used to enhance the polymer conductivity,m aking it suitable for EIS measurements. [48] The monomers served as sensitive layer building blocks with side-chain groups covering ar ange of interac- tions, which were present on the virus surface and BPA. The EIS electrode was prepared as follows:t he monomers were polymerized through the radical polymerization reaction at 70 8Cu ntil agel form was obtained.…”

Section: Mcipsa Nd Mips Eis Gold Electrode Preparationsmentioning

confidence: 99%

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Small‐Molecule Dengue Virus Co‐imprinting and Its Application as an Electrochemical Sensor

et al. 2017

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Polymers can be synthesized to recognize small molecules. This is achieved by introducing the target molecule during monomer self‐assembly, where they can be incorporated during cross‐linking polymerization. Following additional pre‐processing, the material obtained can then be applied as a sensing layer for these molecules in many applications. The sensitivity of the polymers depends on the “active sites” imprinted on the surface. Increasing the number of active sites on the polymers surface can be achieved by using nanoparticles as a platform to support and concentrate the molecules for imprinting. In this work, we report the first use of dengue virus as a supporting nanoparticle to make for a more effective polymer composite sensor for the detection of bisphenol A (BPA), which is an environmental contaminant. The dengue virus has a nanoparticle size of around 100 nm and its surface provides regions where lipids and hydrophobic compounds can bind, making it an ideal support. The mixing of BPA with dengue prior to monomer self‐assembly led to imprinted polymer surfaces with much higher density BPA binding sites and a limit of detection of 0.1 pm. We demonstrate that a BPA–dengue co‐imprinting polymer composite sensor shows a very high sensitivity for BPA, but with lower production costs and technical requirements than other comparable methods.

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A novel method for dengue virus detection and antibody screening using a graphene-polymer based electrochemical biosensor

Cited by 112 publications

References 36 publications

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

Polymers imprinted with three REG1B peptides for electrochemical determination of Regenerating Protein 1B, a urinary biomarker for pancreatic ductal adenocarcinoma

Polymeric Materials for Printed-Based Electroanalytical (Bio)Applications

Small‐Molecule Dengue Virus Co‐imprinting and Its Application as an Electrochemical Sensor

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