A novel method for calculating mean erythrocyte age using erythrocyte creatine

Kameyama, Masashi; Koga, Masafumi; Okumiya, Toshika

doi:10.18632/aging.103193

Cited by 14 publications

(25 citation statements)

References 15 publications

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“…The creatine concentration in the supernatant was measured using the enzymatic assay method. The mean RBC age (days) was − 22.84 × log e (erythrocyte creatine) + 65.83 [6]. Erythrocyte creatine levels represent the average or cumulative erythropoiesis up to the present.…”

Section: Erythrocyte Creatinementioning

confidence: 99%

“…Therefore, erythrocyte creatine levels are indicative of chronic rather than an acute haemolytic conditions. The RBC ages in 305 healthy subjects in our previous study were extracted as healthy controls [5,6].…”

Section: Erythrocyte Creatinementioning

confidence: 99%

“…Compared with the 51 Cr-labelling method, erythrocyte creatine is a simple, rapid, and economically favourable marker that can be measured using a single blood sample examination. Erythrocyte creatine level is considered a quantitative marker of mean RBC age because young RBCs contain substantially higher creatine levels than older RBCs, and creatine contents in RBCs decrease gradually with advancing cell age; an elevated erythrocyte creatine level reflects a shortened RBC age [ 4 – 6 ]. Moreover, in contrast to reticulocyte levels, which reflect present erythropoiesis, erythrocyte creatine levels reflect average or cumulative erythropoiesis up to the present [ 5 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Shortened red blood cell age in patients with end-stage renal disease who were receiving haemodialysis: a cross-sectional study

et al. 2020

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Background The causes of anaemia in patients with end-stage renal disease include a relative deficiency in erythropoietin production and complex clinical conditions. We aimed to investigate the underlying mechanisms of anaemia in patients with end-stage renal disease who were undergoing maintenance dialysis by measuring erythrocyte creatine levels. Methods In a cross-sectional study, we evaluated 69 patients with end-stage renal disease who were receiving haemodialysis (n = 55) or peritoneal dialysis (n = 14). Erythrocyte creatine level, a quantitative marker of mean red blood cell (RBC) age, was measured. Results The mean RBC age was significantly shorter in the haemodialysis group than in the peritoneal dialysis group (47.7 days vs. 59.8 days, p < 0.0001), although the haemoglobin levels were comparable between the groups. A Spearman correlation coefficient analysis revealed that shortened RBC age positively correlated with transferrin saturation (r = 0.54), ferritin level (r = 0.47), and haptoglobin level (r = 0.39) but inversely related with reticulocyte (r = − 0.36), weekly doses of erythropoiesis-stimulating agents (ESAs; r = − 0.62), erythropoietin resistance index (r = − 0.64), and intradialytic ultrafiltration rate (r = − 0.32). Conclusions Shortened RBC age was observed in patients who were receiving maintenance haemodialysis and was associated with iron deficiency, greater haptoglobin consumption, higher ESA requirements, and poor erythropoietin responsiveness, as well as with greater intradialytic fluid extraction.

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Section: Erythrocyte Creatinementioning

confidence: 99%

Section: Erythrocyte Creatinementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Shortened red blood cell age in patients with end-stage renal disease who were receiving haemodialysis: a cross-sectional study

et al. 2020

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“…The relationship between and EC was previously established based on a model and the data 8 from 21 patients, which included EC and , as following 9 : …”

Section: Introductionmentioning

confidence: 99%

Estimation of the hemoglobin glycation rate constant

Kameyama

Okumiya

Tokuhiro

et al. 2021

Sci Rep

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In a previous study, a method of obtaining mean erythrocyte age ($$M_{RBC}$$ M RBC ) from HbA1c and average plasma glucose (AG) was proposed. However, the true value of the hemoglobin glycation constant ($$k_g$$ k g dL/mg/day), required for this model has yet to be well characterized. Another study also proposed a method of deriving $$M_{RBC}$$ M RBC from erythrocyte creatine (EC). Utilizing these formulae, this study aimed to determine a more accurate estimate of $$k_g$$ k g . One hundred and seven subjects including 31 patients with hemolytic anemia and 76 subjects without anemia were included in this study. EC and HbA1c data were analyzed, and $$M_{RBC}$$ M RBC using HbA1c, AG and the newly-derived constant, $$k_g$$ k g were compared to $$M_{RBC}$$ M RBC using traditional $$^{51}\hbox {Cr}$$ 51 Cr in three patients whose data were taken from previous case studies. A value of $$7.0\times 10^{-6}$$ 7.0 × 10 - 6 dL/mg/day was determined for $$k_g$$ k g . $$M_{RBC}$$ M RBC using HbA1c, AG and $$k_g$$ k g were found to no be significantly different (paired t-test, $$p=0.45$$ p = 0.45 ) to $$M_{RBC}$$ M RBC using traditional $$^{51}\hbox {Cr}$$ 51 Cr . $$k_g$$ k g enables the estimation of $$M_{RBC}$$ M RBC from HbA1c and AG.

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“…Erythrocyte creatine level is considered a quantitative marker of mean RBC age because young RBCs contain substantially higher creatine levels than older RBCs, and creatine contents in RBCs decrease gradually with advancing cell age; an elevated erythrocyte creatine level re ects a shortened RBC age [4][5][6]. Moreover, in contrast to reticulocyte levels, which re ect present erythropoiesis, erythrocyte creatine levels re ect average or cumulative erythropoiesis up to the present [5,7].…”

Section: Introductionmentioning

confidence: 99%

Shortened red blood cell age in patients with end-stage renal disease who were receiving haemodialysis: a cross-sectional study

Matsumura

Okumiya

Sugiura

et al. 2020

Preprint

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Background: The causes of anaemia in patients with end-stage renal disease include a relative deficiency in erythropoietin production and complex clinical conditions. We aimed to investigate the underlying mechanisms of anaemia in patients with end-stage renal disease who were undergoing maintenance dialysis by measuring erythrocyte creatine levels.Methods: In a cross-sectional study, we evaluated 69 patients with end-stage renal disease who were receiving haemodialysis (n = 55) or peritoneal dialysis (n = 14). Erythrocyte creatine level, a quantitative marker of mean red blood cell (RBC) age, was measured.Results: The mean RBC age was significantly shorter in the haemodialysis group than in the peritoneal dialysis group (47.7 days vs. 59.8 days, p<0.0001), although the haemoglobin levels were comparable between the groups. A Spearman correlation coefficient analysis revealed that shortened RBC age positively correlated with transferrin saturation (r = 0.54), ferritin level (r= 0.47), and haptoglobin level (r = 0.39) but inversely related with reticulocyte (r = −0.36), weekly doses of erythropoiesis-stimulating agents (ESAs; r = −0.62), erythropoietin resistance index (r = −0.64), and intradialytic ultrafiltration rate (r = −0.32).Conclusions: Shortened RBC age was observed in patients who were receiving maintenance haemodialysis and was associated with iron deficiency, greater haptoglobin consumption, higher ESA requirements, and poor erythropoietin responsiveness, as well as with greater intradialytic fluid extraction.

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A novel method for calculating mean erythrocyte age using erythrocyte creatine

Cited by 14 publications

References 15 publications

Shortened red blood cell age in patients with end-stage renal disease who were receiving haemodialysis: a cross-sectional study

Shortened red blood cell age in patients with end-stage renal disease who were receiving haemodialysis: a cross-sectional study

Estimation of the hemoglobin glycation rate constant

Shortened red blood cell age in patients with end-stage renal disease who were receiving haemodialysis: a cross-sectional study

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