A novel method for biomaterial scaffold internal architecture design to match bone elastic properties with desired porosity

Lin, Cheng-Yu; Kikuchi, Noboru; Hollister, Scott J.

doi:10.1016/j.jbiomech.2003.09.029

Cited by 348 publications

(213 citation statements)

References 29 publications

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“…To tackle this problem, computer-assisted-design and computerassisted-manufacture (CAD/CAM) techniques, a.k.a. solid freeform fabrication (SFF) or rapid prototyping, that have been widely used in modern manufacture industry, are adopted by the field of tissue engineering [3,[95][96][97][98]. However, the existing SFF techniques also have their limitations such as limited material selections, inadequate resolution, and structural heterogeneity due to the "pixel assembly" nature of these fabrication processes [2].…”

Section: Phase Separationmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,199

819

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Section: Phase Separationmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,199

819

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“…Scaffolds with high porosity and desirable mechanical properties for bone implants have been developed by many research groups with varying success [10][11][12][13][14][15]. One new method developed to produce highly oriented, microstructures with varying porosity is freeze-casting.…”

Section: Introductionmentioning

confidence: 99%

Potential Bone Replacement Materials Prepared by Two Methods

et al. 2012

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Natural and synthetic hydroxyapatite (HA) scaffolds for potential load-bearing bone implants were fabricated by two methods. The natural scaffolds were formed by heating bovine cancellous bone at 1325°C, which removed the organic and sintered the HA. The synthetic scaffolds were prepared by freeze-casting HA powders, using different solid loadings (20-35 vol.%) and cooling rates (1-10°C/min). Both types of scaffolds were infiltrated with polymethylmethacrylate (PMMA). The porosity, pore size, and compressive mechanical properties of the natural and synthetic scaffolds were investigated and compared to that of natural cortical and cancellous bone. Prior to infiltration, the sintered cancellous scaffolds exhibited pore sizes of 100 -300 µm, a strength of 0.4 -9.7 MPa, and a Young's modulus of 0.1 -1.2 GPa. The freeze-casted scaffolds had pore sizes of 10 -50 µm, strengths of 0.7 -95

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“…This issue is compounded by increased tissue synthesis at the periphery of the constructs, thereby further restricting diffusion of key chemical cues to the central regions of three dimensional (3D) constructs. Recent advances in both computational topology design (CTD) and solid free-form fabrication (SFF) have made it possible to create scaffolds with well defined architectures (8)(9)(10)(11)(12)(13). The benefits of these technological advancements include the enhancement of interconnected porosity which can improve cell seeding and the incorporation of channels to guide cell migration and tissue ingrowth (14) .…”

Section: Introductionmentioning

confidence: 99%

Engineering of Large Cartilaginous Tissues Through the Use of Microchanneled Hydrogels and Rotational Culture

Buckley

Thorpe

Kelly

2009

Tissue Engineering Part A

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The development of functional engineered cartilaginous tissues of sufficient size that can be used clinically to treat large defects remains a major and significant challenge. This study investigated if the introduction of microchannels into chondrocyte-seeded agarose hydrogels would result in the formation of a superior and more homogenous cartilaginous tissue due to enhanced nutrient transport. Microchannel construct cylinders were fabricated via a moulding process utilising a pillared structure to create the required architecture. Constructs were subjected to either constant rotation in a rotational bioreactor system or free swelling conditions. After 28 days of free swelling culture the presence of microchannels did not enhance GAG accumulation within the core of the construct compared to solid constructs (0.317 ± 0.002 % w/w vs. 0.401 ± 0.020 % w/w). However under dynamically rotating conditions, GAG accumulation in the cores (1.165 ± 0.132 % w/w) of microchannel constructs were similar to that in the periphery (1.23 ± 0.074 % w/w) of solid constructs, although still significantly lower than their corresponding periphery (1.64 ± 0.133 % w/w) after 28 days. These results confirm that cellular nutrient consumption is primarily responsible for creating the spatial gradients in molecules regulating the biosynthetic activity of chondrocytes through the volume of hydrogels, and that changing the scaffold architecture alone may have little effect while the inherent diffusivity of the material remains high. Rather a combination of forced convection and modified scaffold architecture is necessary to engineer large cartilaginous tissues in vitro.

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A novel method for biomaterial scaffold internal architecture design to match bone elastic properties with desired porosity

Cited by 348 publications

References 29 publications

Biomimetic materials for tissue engineering

Biomimetic materials for tissue engineering

Potential Bone Replacement Materials Prepared by Two Methods

Engineering of Large Cartilaginous Tissues Through the Use of Microchanneled Hydrogels and Rotational Culture

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