A Novel Method for ABO Genotyping Using a DNA Chip

Watanabe, Ken; Ikegaya, Hiroshi; Hirayama, Koichi; Motani, Hisako; Iwase, Hirotaro; Kaneko, Hiroto; Fukushima, Hisashi; Akutsu, Tomoko; Sakurada, Koichi

doi:10.1111/j.1556-4029.2010.01630.x

Cited by 15 publications

(11 citation statements)

References 24 publications

(24 reference statements)

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“…The individual identification level of mitochondrial DNA is far below the STR approach [4], but effectively augments the value of other information used for investigations. In addition, DNA methodologies, such as Y-STR typing, single nucleotide polymorphisms, and next-generation sequencing technology have also been applied to various practical tasks [5][6][7]. Due to its reliability, DNA is widely recognized, not only by investigators, but also by the general public, as essential in criminal investigations.…”

Section: Introductionmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

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Section: Introductionmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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“…The genetic method determining the ABO blood group [7,8] is also known. However, discrepancies between phenotypic and genotypic ABO blood groups have been reported [9].…”

Section: Introductionmentioning

confidence: 99%

Involvement of soil bacteria in ABO blood mistyping

Takada

Mori²,

Takai³

et al. 2015

Legal Medicine

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“…For example, in 2011, Lee et al [19] announced a rapid direct ABO genotyping method that eliminates the need for DNA extraction, using a multiplex allele-specific primer set for the simultaneous detection of 3 single nucleotide polymorphism (SNP) sites. In 2011, another group, Watanabe et al [20], developed a simple method for ABO genotyping using a DNA chip. This method is suitable for the simultaneous analysis of ABO genotyping and primate-specific species identification, especially to identify the ABO type of decomposed samples.…”

Section: Discovery and Nomenclature Of The Abo Blood Groupsmentioning

confidence: 99%

Genetic Kinship Investigation from Blood Groups to DNA Markers

Geserick¹,

Wirth

2012

Transfus Med Hemother

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The forensic application of hereditary characteristics became possible after the discovery of human blood groups by Karl Landsteiner in 1901. The foundation for their use in kinship investigation was laid by Emil von Dungern and Ludwig Hirschfeld in 1910 by clarification of the inheritance of the ABO groups. Up to the middle of the 20th century further red cell membrane systems were discovered. From the 1920s Fritz Schiff and Georg Strassmann fought for the introduction of blood groups into forensic kinship investigation. A new era of hemogenetics was opened from 1955 as genetic polymorphisms were described in serum proteins. Starting in 1958 there followed the complex HLA system of white blood cells, which from 1963 was joined by polymophisms in erythrocyte enzymes. Therefore, from the 1980s, it was possible to clarify the majority of kinship cases with a combination of conventional markers. From 1990 to 2000 the conventional markers were gradually replaced by the more effective DNA markers. Simultaneously typing shifted from the phenotype level to the genotype level. The genomic structure of con ventional genetic markers could also now be explained. As a reflection of scientific progress the legal situation also changed, particularly in the form of the official guidelines for kinship investigation.

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A Novel Method for ABO Genotyping Using a DNA Chip

Cited by 15 publications

References 24 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Involvement of soil bacteria in ABO blood mistyping

Genetic Kinship Investigation from Blood Groups to DNA Markers

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