A novel member of murine Polycomb-group proteins, Sex comb on midleg homolog protein, is highly conserved, and interacts with RAE28/mph1 in vitro

Tomotsune, Daihachiro; Takihara, Yoshihiro; Berger, Joel; Duhl, David M.; Joo, Sunghae; Kyba, Michael; Shirai, Makoto; Ohta, Hiroyuki; Matsuda, Yoichi; Honda, Barry M.; Simon, John; Shimada, Kazunori; Brock, Hugh W.; Randazzo, Filippo

doi:10.1046/j.1432-0436.1999.6540229.x

Cited by 39 publications

(33 citation statements)

References 54 publications

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“…Drosophila PRC1 contains the core subunits Polycomb (Pc), Polyhomeotic (Ph), Posterior sex combs (Psc) and Sex combs extra (Sce)/Ring1 12,51,52 ( Table 1). Several observations suggest that Scm also associates with PRC1 albeit not as a stoichiometric subunit: Biochemical purifications of PRC1 contain substoichiometric amounts of Scm, 52,53 Scm interacts with the PRC1 subunit Ph in vitro 37,54 and can stably assemble into a reconstituted PRC1 complex. 55 However, co-immunoprecipitations failed to confirm a robust interaction between Scm and PRC1 components and the bulk of Scm in embryo extract separates from Ph in gel filtration analysis.…”

Section: Mbt Complexesmentioning

confidence: 99%

Chromatin regulation: How complex does it get?

Meier¹,

Brehm

2014

Epigenetics

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Section: Mbt Complexesmentioning

confidence: 99%

Chromatin regulation: How complex does it get?

Meier¹,

Brehm

2014

Epigenetics

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“…PcG proteins associate with each other through conserved domains and form multimeric complexes (Alkema et al, 1997;Hashimoto et al, 1998;Takihara and Hara, 2000;Tomotsune et al, 1999). There exist at least two classes of PcG complexes, PcG complexes 1 and 2 (Takihara and Hara, 2000).…”

mentioning

confidence: 99%

“…There exist at least two classes of PcG complexes, PcG complexes 1 and 2 (Takihara and Hara, 2000). PcG complex 1 consists of multimeric complexes, including PcG family members, M33, bmi1, mel18, and rae28 (also designated as mph1), mph2, and Scmh1 (Alkema et al, 1997;Hashimoto et al, 1998;Tomotsune et al, 1999) and may be a homolog to Drosophila Polycomb repressive complex 1, which competitively antagonizes the ATP-dependent nucleosome remodeling ability of SWI/SNF family complexes (Shao et al, 1999). PcG complex 2 is distinct from PcG complex 1 and includes eed and Enx1/EZH2 or Enx2 (Takihara and Hara, 2000).…”

mentioning

confidence: 99%

Overexpression of Polycomb-Group Gene rae28 in Cardiomyocytes Does Not Complement Abnormal Cardiac Morphogenesis in Mice Lacking rae28 But Causes Dilated Cardiomyopathy

Koga

Kaji

Nishii

et al. 2002

Laboratory Investigation

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SUMMARY:The Polycomb-group genes (PcG) are widely conserved from Drosophila to mammals and are required for maintaining positional information during development. The rae28 gene (rae28) is a member of the mouse PcG. Mice deficient in rae28 (rae28 Ϫ/Ϫ ) demonstrated that rae28 has a role not only in anteroposterior patterning but also in cardiac morphogenesis. In this study we generated transgenic mice with ubiquitous or cardiomyocyte-specific exogenous rae28 expression. Genetic complementation experiments with these transgenic mice showed that ubiquitous expression of rae28 could reverse the cardiac anomalies in rae28 Ϫ/Ϫ , whereas cardiomyocyte-specific expression of rae28 could not, suggesting that rae28 is involved in cardiac morphogenesis through a noncardiomyocyte pathway. Interestingly, however, cardiomyocyte-specific overexpression of rae28 caused dilated cardiomyopathy, which was associated with cardiomyocyte apoptosis, abnormal myofibrils, and severe heart failure. Cardiac expression of rae28 was predominant in the early embryonic stage, whereas that of the other PcG members was relatively constitutive. Because rae28 forms multimeric complexes with other PcG proteins in the nucleus, it is presumed that constitutive cardiomyocyte-specific rae28 overexpression impaired authentic PcG functions in the heart. rae28-induced dilated cardiomyopathy may thus provide a clue for clarifying the direct role of PcG in the maintenance of cardiomyocytes. (Lab Invest 2002, 82:375-385).

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“…The sex comb on midleg like-2 (SCML2) gene is one of the four homologs of Scm (the others being SCML1, SCM homolog 1 and SCM-like with four MBT domains) in mammals (7)(8)(9)(10)(11). SCML2 has been identified as a human gene in the Xp22 region that encodes a protein of 700 amino acids (7).…”

Section: Introductionmentioning

confidence: 99%

Sex comb on midleg like-2 is a novel specific marker for the diagnosis of gastroenteropancreatic neuroendocrine tumors

Yang

Huang

Xiao

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Sex comb on midleg like-2 (SCML2) is a polycomb-group protein that encodes transcriptional repressors essential for appropriate development in the fly and in mammals. On the basis of previous findings, the present study aimed to explore the possibility of developing SCML2 into a new diagnostic marker for gastroenteropancreatic neuroendocrine tumors (GEP-NETs). A total of 64 paired GEP-NET tissues and adjacent non-tumorous tissues were obtained from patients who had undergone surgical resection between January 2009 and January 2014, and the expression of SCML2 and two neuroendocrine markers, namely synaptophysin (Syn) and chromogranin A (CgA), in the tissues was assessed by immunohistochemistry. Strong SCML2 staining was observed predominantly in the cell nuclei of GEP-NET tissues, and the overall expression rate and staining intensity of SCML2 were higher than those of Syn or CgA, respectively. Spearman rank correlation analysis demonstrated that SCML2 was not correlated with either Syn or CgA, while the combined detection of SCML2 with Syn or with CgA increased the diagnostic sensitivity to 100%. SCML2 expression in GEP-NETs was associated with several clinicopathological parameters, such as histological type, tumor grade, depth of invasion and clinical stage. Kaplan-Meier survival curves revealed that patients with higher SCML2 expression had lower survival rates than those with lower expression levels, while Cox proportional hazards regression analysis revealed that SCML2 was not an independent prognostic factor for GEP-NET patients. Therefore, SCML2 may have potential as a specific marker for joint use with other markers to improve the diagnostic efficiency of GEP-NETs.

show abstract

A novel member of murine Polycomb-group proteins, Sex comb on midleg homolog protein, is highly conserved, and interacts with RAE28/mph1 in vitro

Cited by 39 publications

References 54 publications

Chromatin regulation: How complex does it get?

Chromatin regulation: How complex does it get?

Overexpression of Polycomb-Group Gene rae28 in Cardiomyocytes Does Not Complement Abnormal Cardiac Morphogenesis in Mice Lacking rae28 But Causes Dilated Cardiomyopathy

Sex comb on midleg like-2 is a novel specific marker for the diagnosis of gastroenteropancreatic neuroendocrine tumors

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