Abstract:Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 22 R EVERSE genetic approach was used to isolate and characterize Medicago truncatula containing "knockout" mutations in gene involved in nodulation process and in many other physiological processes. More than 60 Tnt1-Flanking sequence tags (FSTs) ranging from ~70bp to ~600bp were isolated, sequenced and submitted to Genebank referring for akt1 mutant characterization. The proper Tnt1-insertion was mapped in chromosome number 8 of Medicago truncatula geno… Show more
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