A novel mechanism of rs763110 polymorphism contributing to cervical cancer risk by affecting the binding affinity of C/EBPβ and OCT1 complex to chromatin

Wu, Shenshen; Wang, Shizhi; Fu, Yuli; Tang, Weiyan; Jin, Hua; Meng, Qingtao; Zhang, Chengcheng; Cui, Mengjing; Cao, Xiaoli; Li, Xiaobo; Zhang, Zhengdong; Chen, Rui

doi:10.1002/ijc.30490

Cited by 18 publications

(12 citation statements)

References 30 publications

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“…Further siRNA knockdown experiments showed that Krox-20 was recruited to Sp1 binding motif in both HeLa (HPV18–positive), SiHa (HPV16–positive), and C–33A (HPV–negative) cervical cancer cells in vitro knockdown experiments. This also confirmed that HPVs infection is not a necessary Etiological Factor for cervical cancer proposed by Wu et al [ 22 ].…”

Section: Discussionsupporting

confidence: 88%

XRCC1 mediated the development of cervival cancer through a novel Sp1/Krox-20 swich

et al. 2017

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Cervical cancer is the second leading cause of mortality among women. Impairment of the base excision repair (BER) pathway is one of the major causes of the initiation and progression of cervical cancer. However, whether the polymorphisms of the BER pathway components (i.e., HOGG1, XRCC1, ADPRT, and APE1) can affect the risk of cervical cancer remains unknown. Herein, we applied a hospital-based case-control study covering two independent cohorts and a subsequent functional assay to determine the roles of the single nucleotide polymorphisms (SNPs) of the BER pathway genes in cervical cancer. Results indicated that the XRCC1 rs3213245 (-77TC) TT genotype was associated with an increased risk of cervical cancer. The immunohistochemistry assay showed that XRCC1 protein expression levels were upregulated in cervical cancer patients with the XRCC1 rs3213245 CC genotype compared with the CT or TT genotypes. Further, results from ChIP assay showed that Sp1 could bind to the −77 site and that the rs3213245 C genotype promoted the binding of Sp1 to the XRCC1 promoter. Moreover, ChIP/Re-ChIP assays revealed that transcription factor Krox-20 was recruited to the XRCC1 rs3213245 mutation region and regulated the transcription of the XRCC1 gene by interacting with Sp1, ultimately mediated cervical cancer development. In summary, the findings indicated that the functional XRCC1 SNP rs3213245 was associated with the risk of cervical cancer based on the Sp1/Krox-20 switch.

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Section: Discussionsupporting

confidence: 88%

XRCC1 mediated the development of cervival cancer through a novel Sp1/Krox-20 swich

et al. 2017

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“…21 Briefly, the following antibodies were used for the immunoprecipitation reaction: Sp1, NF1, positive control RNA poly (Active Motif, Carlsbad, CA, USA) or negative control IgG (Active Motif, Carlsbad, CA, USA). 21 Briefly, the following antibodies were used for the immunoprecipitation reaction: Sp1, NF1, positive control RNA poly (Active Motif, Carlsbad, CA, USA) or negative control IgG (Active Motif, Carlsbad, CA, USA).…”

Section: Chromatin Immunoprecipitation Assay (Chip)mentioning

confidence: 99%

“…Human peripheral white blood and colorectal cancer cells were used for ChIP assay with a ChIP-IT TM Express Magnetic Assay kit and ChIP-IT TM Control qPCR kit (Active Motif, Carlsbad, CA, USA) as described previously. 21 Briefly, the following antibodies were used for the immunoprecipitation reaction: Sp1, NF1, positive control RNA poly (Active Motif, Carlsbad, CA, USA) or negative control IgG (Active Motif, Carlsbad, CA, USA). The precipitated genomic DNA was analyzed by quantitative RT-PCR with the following DR4 promoter primers including the rs13278062 polymorphism: 5 0 -CCTCAGCCTTTCTGTGACCC-3 0 (forward) and 5 0 -AAATCGCTTGAACCTGGGAG-3 0 (reverse).…”

Section: Chromatin Immunoprecipitation Assay (Chip)mentioning

confidence: 99%

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DR4 mediates the progression, invasion, metastasis and survival of colorectal cancer through the Sp1/NF1 switch axis on genomic locus

Meng

Zhang

et al. 2018

Intl Journal of Cancer

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The single nucleotide polymorphism (SNP), -397G > T (rs13278062) polymorphism, in the promoter of Death Receptor 4 (DR4) had been reported to be associated with a significantly increased risk for bladder cancer. However, the association of this SNP with the risk of colorectal cancer has not been reported. In this study, we performed a case-control study in 1,078 colorectal cancer patients and 1,175 matched healthy controls to evaluate the association of the potential functional genetic variants in DR4 with risk and survival of colorectal cancer. PCR-TaqMan were used to genotype the rs13278062, rs1000294 and rs2235126 polymorphisms. We found that subjects carrying the rs13278062 GT/TT genotypes had a significantly lower risk and increased survival time when compared to the GG genotype. We also constructed the rs13278062 GT/TT genotype in SW480 and SW620 cells (rs13278062 is GG in both cell lines) with the CRISPR/Cas9 system. Flow cytometry experiments showed that the rs13278062 TT genotype promoted apoptosis in colorectal cancer cells. In vitro and in vivo experiments established that the rs13278062 G to T mutation inhibited carcinogenesis and metastasis of colorectal cancer. Chromatin immunoprecipitation (ChIP) assays revealed that the rs13278062 G > T polymorphism altered the binding affinity of the transcription factors Sp1/NF1 to the rs13278062 mutation region. Immunohistochemistry, western blot, and qPCR corroborated that the rs13278062 GT/TT genotypes increased the expression of DR4 protein in colorectal cancer tissues and cells. In conclusion, these findings indicate that DR4 mediated progression, invasion, metastasis and survival of colorectal cancer via the Sp1/NF1 switch axis on genomics locus.

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“…It is estimated that approximately 500,000 new cases of cervical cancer are reported annually and about 230,000 women die of cervical cancer annually [4] . Cervical cancer is a complex disease involving the abnormal expression of many oncogenes and tumor suppressor genes [5] , [6] , [7] , [8] . Previous studies have revealed several genes associated with human cervical cancer [9] , [10] , [11] , [12] , [13] .…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-30e Functions as a Tumor Suppressor in Cervical Carcinoma Cells through Targeting GALNT7

Liu

et al. 2017

Translational Oncology

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Cervical cancer is the third most common cancer in women worldwide. However, the underlying mechanism of occurrence and development of cervical cancer is obscure. In this study, we observed that miR-30e was downregulated in clinical cervical cancer tissues and cervical cancer cells. Next, overexpression of miR-30e reduced the cervical cancer cell growth through MTT, colony formation, EdU, and Transwell assay in SiHa and Caski cells. Subsequently, UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7 (GALNT7) was identified as a potential miR-30e target by bioinformatics analysis. Moreover, we showed that miR-30e was able to bind to the 3′UTR of GALNT7 by luciferase reporter assay. In addition, the mRNA and protein levels of GALNT7 in cervical cancer cells were downregulated by miR-30e. And we validated that downregulation of GALNT7 repressed the proliferation of SiHa and Caski cells by MTT, colony formation, and Transwell assay. We identified that the restoration of GALNT7 expression was able to counteract the effect of miR-30e on cell proliferation of cervical cancer cells. Furthermore, we found that the expression levels of GALNT7 were frequently upregulated and negatively correlative to those of miR-30e in cervical cancer tissues. In addition, we validated that restoration of GALNT7 rescued the miR-30e–suppressed growth of cervical cancer xenografts in vivo. In conclusion, the current results suggest that miR-30e may function as tumor suppressors in cervical cancer through downregulation of GALNT7. Both miR-30e and its novel target, GALNT7, may play an important role in the process of cervical cancer.

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A novel mechanism of rs763110 polymorphism contributing to cervical cancer risk by affecting the binding affinity of C/EBPβ and OCT1 complex to chromatin

Cited by 18 publications

References 30 publications

XRCC1 mediated the development of cervival cancer through a novel Sp1/Krox-20 swich

XRCC1 mediated the development of cervival cancer through a novel Sp1/Krox-20 swich

DR4 mediates the progression, invasion, metastasis and survival of colorectal cancer through the Sp1/NF1 switch axis on genomic locus

MicroRNA-30e Functions as a Tumor Suppressor in Cervical Carcinoma Cells through Targeting GALNT7

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