A novel mechanism of Korean Red Ginseng-mediated anti-inflammatory action via targeting caspase-11 non-canonical inflammasome in macrophages

Min, Ji‐Hyun; Cho, Hui-Jin; Yi, Young-Su

doi:10.1016/j.jgr.2021.12.009

Cited by 24 publications

(16 citation statements)

References 57 publications

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“…Investigation into the ginsenoside-mediated anti-inflammatory effects of KRG have mainly focused on the priming process and canonical inflammasomes of the triggering process [ 13 , 14 , 15 ]. Our previous study reported a new mechanism by which KRG exerts anti-inflammatory action and ameliorates lethal sepsis in mice through inhibiting caspase-11 non-canonical inflammasome in macrophages [ 16 ]; however, the anti-inflammatory constituents in KRG that inhibit caspase-11 non-canonical inflammasome remain unknown. The present study is the first to demonstrate that KRGSF is a key anti-inflammatory factor in caspase-11 noncanonical inflammasome activation during macrophage-mediated inflammatory responses.…”

Section: Discussionmentioning

confidence: 99%

“…Another principal consequence of inflammasome activation is the secretion of pro-inflammatory cytokines and inflammatory mediators through the GSDMD pores of pyroptotic cells [ 12 , 16 ]. KRGSF decreased the expression and secretion of IL-1β and IL-18 in caspase-11 non-canonical inflammasome-activated J774A.1 cells, but diclofenac showed less and no inhibitory effect, respectively, indicating that KRGSF has better inhibitory potential in mRNA expression and secretion of pro-inflammatory cytokines in caspase-11 non-canonical inflammasome-activated macrophages with a mechanism distinct from that of diclofenac.…”

Section: Discussionmentioning

confidence: 99%

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Korean Red Ginseng Saponins Play an Anti-Inflammatory Role by Targeting Caspase-11 Non-Canonical Inflammasome in Macrophages

Cho

Kim

2023

IJMS

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We previously reported that Korean red ginseng (KRG) exerts an anti-inflammatory role through inhibiting caspase-11 non-canonical inflammasome in macrophages; however, the components responsible for the anti-inflammatory role remained unclear. This study explored the anti-inflammatory activity of the KRG saponin fraction (KRGSF) in caspase-11 non-canonical inflammasome-activated macrophages. KRGSF inhibited pyroptosis, pro-inflammatory cytokine secretion, and inflammatory mediator production in caspase-11 non-canonical inflammasome-activated J774A.1 cells. A mechanism study revealed that KRGSF-induced anti-inflammatory action was mediated via suppressing the proteolytic activation of caspase-11 and gasdermin D (GSDMD) in caspase-11 non-canonical inflammasome-activated J774A.1 cells. Moreover, KRGSF increased the survival of lethal septic mice. Taken together, these results reveal KRGSF-mediated anti-inflammatory action with a novel mechanism, by inhibiting caspase-11 non-canonical inflammasome in macrophages.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Korean Red Ginseng Saponins Play an Anti-Inflammatory Role by Targeting Caspase-11 Non-Canonical Inflammasome in Macrophages

Cho

Kim

2023

IJMS

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“…LDH secretion was assayed using a kit according to the manufacturer’s protocol (BCT-LDHP, Biomax, Seoul, Korea). The sup was transferred to a 96-well plate, and the reagent was added to each well and incubated for 30 min [ 28 ]. The absorbance signal was measured at 490 nm using a multi-microplate spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

et al. 2022

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Maltol (3-hydroxy-2-methyl-4-pyrone) is used widely as a food and cosmetic supplement, and it has antioxidant and anti-inflammatory activities. Inflammasome causes the maturation and secretion of interleukin (IL)-1β and -18 through the activation of caspase-1 (Casp1), which contributes to various inflammatory diseases. This study examined the effects of maltol on the inflammasome activation in macrophages and mice. Lipopolysaccharide (LPS)-primed macrophages were treated with a trigger of NLRP3, NLRC4, AIM2, or non-canonical (NC) inflammasomes in the presence of maltol. The secretion of IL-1β and IL-18 and the cleavage of Casp1 were analyzed as indices of inflammasome activation. Mice were injected with LPS and an NLRP3 trigger with or without maltol, and the peritoneal IL-1β secretions were observed. The effects of maltol on reactive oxygen species (ROS) production and Casp1 activity were analyzed to determine the mechanism. Maltol inhibited the activation of NLRP3 and NC inflammasomes, but it did not alter the other inflammasomes. Maltol also attenuated IL-1β secretion resulting from the inflammasome activation in mice. The anti-inflammatory mechanism of maltol was revealed by the inhibition of ROS production and Casp1 activity. Maltol is suggested to be promising as a anti-inflammasome molecule.

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“…Korea red ginseng (KRG) has immunomodulatory [ 28 , 31 , 32 ], anti-inflammatory [ [32] , [33] , [34] , [35] , [36] ], and anti-proliferative effects [ 37 , 38 ]. It can maintain immune system homeostasis and enhance resistance to microbial attack by regulating the immune system.…”

Section: Ginseng and Endometriosismentioning

confidence: 99%

Protective role of ginseng in endometriosis during covid-19

Song

Lee

2023

Journal of Ginseng Research

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A novel mechanism of Korean Red Ginseng-mediated anti-inflammatory action via targeting caspase-11 non-canonical inflammasome in macrophages

Cited by 24 publications

References 57 publications

Korean Red Ginseng Saponins Play an Anti-Inflammatory Role by Targeting Caspase-11 Non-Canonical Inflammasome in Macrophages

Korean Red Ginseng Saponins Play an Anti-Inflammatory Role by Targeting Caspase-11 Non-Canonical Inflammasome in Macrophages

Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

Protective role of ginseng in endometriosis during covid-19

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