A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine

Abstract

The presence of an α-1,6-glucosaccharide enhances absorption of water-soluble quercetin glycosides, a mixture of quercetin-3-O-β-d-glucoside (Q3G, 31.8%), mono (23.3%), di (20.3%) and more d-glucose adducts with α-1,4-linkage to a d-glucose moiety of Q3G, in a ligated small intestinal loop of anesthetized rats. We prepared α-1,6-glucosaccharides with different degrees of polymerization (DP) enzymatically and separated them into a megalo-isomaltosaccharide-containing fraction (M-IM, average DP=11.0) and an oligo-isomaltosaccharide-containing fraction (O-IM, average DP=3.6). Luminal injection of either saccharide fraction promoted the absorption of total quercetin-derivatives from the small intestinal segment and this effect was greater for M-IM than O-IM addition. M-IM also increased Q3G, but not the quercetin aglycone, concentration in the water-phase of the luminal contents more strongly than O-IM. The enhancement of Q3G solubilization in the luminal contents may be responsible for the increases in the quercetin glucoside absorption promoted by α-1,6-glucosaccharides, especially that by M-IM. These results suggest that the ingestion of α-1,6-glucosaccharides promotes Q3G bioavailability.

Characterisation of water-soluble proanthocyanidins of Pyracantha fortuneana fruit and their improvement in cell bioavailable antioxidant activity of quercetin

“…In the former studies, it has shown that the bioavailability of quercetin glycoside was promoted by the ingestion of a-1,6-glucosaccharides, this was because the supplementation of a-1,6-glucosaccharides could enhance the content of Q in the water-phase fraction (Shinoki et al, 2013). Another previous study indicated that it could influence on the plasma kinetics of hypericin in the presence of procyanidin B2 and hyperoside, which contains six phenolic hydroxyls at its molecular skeleton.…”

A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, α‐glucosyl‐isoquercitrin, on glucagon‐like peptide 1 (GLP‐1) secretion in rat intestine and enteroendocrine cells

“…B). FOS has shown to increase absorption and bioavailability of Q3G , which might be involved in the enhancement of Q3G‐induced GLP‐1 secretion. The detailed mechanism needs to be further examined.…”

Effects of stachyose on absorption and transportation of tea catechins in mice: possible role of Phase II metabolic enzymes and efflux transporters inhibition by stachyose

“…Interestingly, previous research has suggested that non-digestible oligosaccharides can enhance intestinal absorption of polyphenols, which is possibly due to the fact that non-digestible oligosaccharides increase water-solubility of flavonoids and inhibit their degradation in gut (10, 28). It is also widely recognized that TC can undergo Phase II biotransformation mainly including UGT and SULT in intestinal epithelial cells, which may greatly contribute to their first-pass metabolism (14, 27, 29).…”