A Novel MALDI Matrix for Analyzing Peptides and Proteins: Paraffin Wax Immobilized Matrix

Abstract

A new kind of MALDI matrix, termed paraffin wax immobilized matrix, was used to study peptide mixtures and proteins. During the preparation process, the paraffin wax was heated and coated on the stainless-steel target plate, and then 2,5-dihydrobenzoic acid (DHB) was deposited on the paraffin layer and stainless-steel target plate to obtain different kinds of matrix spots. The morphology of matrices on different supports and peptide-matrix co-crystallization were observed by a high resolution digital-video microscopy system. Peptide mixtures and bovine serum albumin (BSA) digests were used to investigate the performance of the immobilized matrices on the paraffin target. The MALDI-FTMS analysis results also showed that the detection sensitivity of matrices immobilized in the paraffin sample support was better than that on other sample supports.

12-Tungstophosphoric Acid Anchored to Zeolite Hβ: Synthesis, Characterization, and Biodiesel Production by Esterification of Oleic Acid with Methanol

“…A sharp XRD peak at 2θ equal to 26° corresponds to the characteristic peak of TPA ions was observed in the case of TPA 3 /Hβ. This confirms the retention of Keggin unit after supporting on to Hβ supercages …”

“…This confirms the retention of Keggin unit after supporting on to Hβ supercages. 24 The data for surface area, pore width, and NH 3 -TPD are presented in Table 2. Specific surface area and pore diameter strongly decreased for TPA-containing Hβ relative to the starting support.…”

Efficient synthesis of biodiesel over a recyclable catalyst comprising a monolacunary silicotungstate and zeolite Hβ

“…The reection at 2q ¼ 29 indicate the presence of the crystalline phase of SiW 11 on to the support. 27 The catalyst shows reections of SiW 11 with very low intensity indicating a well dispersion of SiW 11 inside the channels of the support. 28 The SEM images show that the surface morphology of 30% SiW 11 /Hb (Fig.…”