A novel maize-infecting mastrevirus from La Réunion Island

Pande, Daniel; Kraberger, Simona; Lefeuvre, Pierre; Lett, Jean‐Michel; Shepherd, Dionne N.; Varsani, Arvind; Martin, Darren P.

doi:10.1007/s00705-012-1314-y

Cited by 11 publications

(2 citation statements)

References 27 publications

(19 reference statements)

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“…This result breaks long-accepted conventions regarding begomoviruses and monocotyledonous plants. Nucleotide sequences from dicotyledon-infecting mastreviruses showed phylogenetic differences from previously reported mastreviruses ( Pande et al, 2012 ). Dicotyledon-infecting mastreviruses are believed to be the result of host adaptation of monocotyledon-infecting mastreviruses ( Liu et al, 1998 ).…”

Section: Discussioncontrasting

confidence: 64%

Tomato Yellow Leaf Curl Virus Infection in a Monocotyledonous Weed (Eleusine indica)

et al. 2021

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Tomato yellow leaf curl virus (TYLCV) is one of the most important plant viruses belonging to the genus Begomovirus of the family Geminiviridae. To identify natural weed hosts that could act as reservoirs of TYLCV, 100 samples were collected at a TYLCV-affected tomato farm in Iksan from 2013 to 2014. The sample weeds were identified as belonging to 40 species from 18 families. TYLCV was detected in 57 samples belonging to 28 species through polymerase chain reaction using root samples including five species (Eleusine indica, Digitaria ciliaris, Echinochloa crus-galli, Panicum dichotomiflorum, and Setaria faberi) from the family Poaceae. Whitefly Bemisia tabaci-mediated TYLCV transmission from TYLCV-infected E. indica plants to healthy tomatoes was confirmed, and inoculated tomatoes showed typical symptoms, such as leaf curling and yellowing. In addition, TYLCV was detected in leaf and root samples of E. indica plants inoculated by both whitefly-mediated transmission using TYLCV-viruliferous whitefly and agro-inoculation using a TYLCV infectious clone. The majority of mastreviruses infect monocotyledonous plants, but there have also been reports of mastreviruses that can infect dicotyledonous plants, such as the chickpea chlorotic dwarf virus. No exception was reported among begomoviruses known as infecting dicots only. This is the first report of TYLCV as a member of the genus Begomovirus infecting monocotyledonous plants.

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Section: Discussioncontrasting

confidence: 64%

Tomato Yellow Leaf Curl Virus Infection in a Monocotyledonous Weed (Eleusine indica)

et al. 2021

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“…Currently, there are 29 recognized members belonging to the genus Mastrevirus based on ICTV classification [ 2 ]. Maize streak Reunion virus (MSRV) is an emerging new member of genus Mastrevirus that has been reported only in La Reunion Island and Nigeria and shares less than 57 % genome-wide identity with all other known mastreviruses [ 4 , 5 ]. Apart from Maize streak virus (MSV), MSRV is the only mastrevirus species detected in maize.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a maize-associated mastrevirus in China by deep sequencing small RNA populations

Chen

Huang

et al. 2015

Virol J

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BackgroundMaize streak Reunion virus (MSRV) is a member of the Mastrevirus genus in the family Geminiviridae. Of the diverse and increasing number of mastrevirus species found so far, only Wheat dwarf virus and Sweetpotato symptomless virus 1 have been discovered in China. Recently, a novel, unbiased approach based on deep sequencing of small interfering RNAs followed by de novo assembly of siRNA, has greatly offered opportunities for plant virus identification.MethodsSamples collected from maize leaves was deep sequencing for virus identification. Subsequently, the assay of PCR, rolling circle amplification and Southern blot were used to confirm the presence of a mastrevirus.ResultsMaize streak Reunion virus Yunnan isolate (MSRV-[China:Yunnan 06:2014], abbreviated to MSRV-YN) was identified from maize collected from Yunnan Province, China, by small RNA deep sequencing. The complete genome of this virus was ascertained as 2,880 nucleotides long by conventional sequencing. A phylogenetic analysis showed it shared 96.3 % nucleotide sequence identity with the isolate of Maize streak Reunion virus from La Reunion Island. To our knowledge, this is the first identification of MSRV in China. Analyses of the viral derived small interfering RNAs (vsiRNAs) profile showed that the most abundant MSRV-YN vsiRNAs were 21, 22 and 24 nt long and biased for A and G at their 5’ terminal residue. There was a slightly higher representation of MSRV-YN siRNAs derived from the virion-sense strand genome than the complementary-sense strand genome. Moreover, MSRV-YN vsiRNAs were not uniformly distributed along the genome, and hotspots were detected in the movement protein and coat protein-coding region.ConclusionsA mastrevirus MSRV-YN collected in Yunnan Province, China, was identified by small RNA deep sequencing. This vsiRNAs profile derived from MSRV-YN was characterized, which might contribute to get an insight into the host RNA silencing defense induced by MSRV-YN, and provide guidelines on designing antiviral strategies using RNAi against MSRV-YN.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-015-0384-3) contains supplementary material, which is available to authorized users.

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