A novel luminometer for rapid antimicrobial susceptibility tests on gram-positive cocci by ATP bioluminescence

Abstract

Summary. The susceptibility of 130 clinical isolates of gram-positive cocci to a wide range of antimicrobial agents was assessed by ATP bioluminescence in a 4-h test. ATP assays were performed on a novel luminometer, the Amerlite Analyser, which measures luminescence from microtitration trays. For most organisms tested, there was good correlation (>90%) with conventional MIC values estimated on 18-h cultures. However, a problem was found with detection of penicillin resistance in Staphylococcusaureus by the ATP method, 13% of strains showing major disagreement. Methicillin resistance of S. aureus was shown reliably for most strains (94%) by ATP assay, provided they were incubated at 30°C. The Amerlite Analyser offers the potential for the development of a semi-automated antimicrobial susceptibility test, with a significant reduction in reagent costs when compared with previously described bioluminescence protocols.

Rapid Antimicrobial Susceptibility Determination of Uropathogens in Clinical Urine Specimens by Use of ATP Bioluminescence

“…Bioluminescence methods have been shown to be useful for the rapid (2-to 4-h) determination of the antibiotic (15,27,31,32). The cutoff for susceptibility was determined to be Ͻ40% of the RLU measurement obtained for bacteria in the absence of antibiotic (15).…”

“…The bacterial ATP bioluminescence methodology has been shown to be useful for the rapid (2-to 4-h) determination of the antibiotic susceptibilities of cultured clinical isolates (15,27,31,32). In this study, we evaluated the analytic validity of bioluminescent antibiotic susceptibility testing of uropathogens in clinical urine specimens.…”

A two-hour antibiotic susceptibility test by ATP-bioluminescence

“…[5][6][7] Given that a reduction of the time needed to perform an AST has been achieved, our method would improve patients' outcome, 2 could decrease the health care expenditure 18 and may delay the rise of bacterial resistances. 1 Wheat et al 7 tested 76 clinical isolates of Enterobacteriaceae to Ampicillin, Piperacillin and Gentamicin by ATP bioluminescence.…”

“…Moreover, non-fermenting gramnegative rods (NFGNR) still remain to be tested. [5][6][7] In this context, the aim of this work was to perform a rapid and complete AST using a firefly bioluminescent ATP assay in which the most predominant bacteria isolated in Clinical Microbiology laboratories were tested against some used antibiotics in clinical practice.…”

Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology

“…Direct testing of killing activity is possible but cumbersome, and both the sensitivity and dynamic range can be limited. Therefore we adapted a luminescence inhibition assay that has been used previously for a variety of bacteria and antimicrobial agents [12][13][14][15][16] , can be performed in small volumes and is rapid (giving a readout of activity within 30 min or less), sensitive and quantitative 8,15 . This assay relies on the genes expressed from a luxCDABE cassette.…”