A novel low-molecular weight alkaline mannanase from Streptomyces tendae

Abstract

A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular mannanase was purified with 26.3% yield using a Sepharose Cl-6B column. The molecular mass of MnSt was approximately 24 kDa. MnSt was stable over a broad pH range (5 ~ 12.5), was thermally stable at 60°C, and functioned optimally at 50°C and a pH of 12.0. MnSt had K m and V max values of 0.05 ± 1 mg/mL and 439 ± 0.5 mmol/min, respectively, using bean gum galactomannan as a substrate. The N-terminal sequence of MnSt was GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis of the MnSt hydrolysis products suggested that the major oligosaccharide produced was mannobiose. MnSt activity was remarkably affected by metal ions, modulators, chelators, and detergents. MnSt was simple to purify, had high thermal stability, was stable over a broad pH range, and produced mannooligosaccharides. MnSt has high potential for use as an industrial biocatalyst, particularly as a bio-bleaching agent or for oligosaccharide production.

Characterization of mannanase-producing bacteria from sago hump

“…The same optimum pH has been reported by Titapoka et al [46]; Mabrouk and Ahwany [28]; Mou et al [33]; Kim et al [24]; Chandra et al [9] and Zhang et al [39]. Meanwhile, Lu et al [27]; Yoo et al [52]; Yang et al [51] and Wang et al [50] reported different optimum pH at 11, 4, 5.5 and 5.…”

“…While Cu 2+ , Zn 2+ and Ni 2+ ions only decrease the activity of mannanase up to 20-30%. The property of Co 2+ ions as activators have been reported by Yoo et al [52] who reported that increasing of activity of mannanase from S. tendae was up to 63%. Yang et al [51] reported that the Co 2+ ions enhanced the activity of mannanase from Neosartorya fischeri up to 10-13%.…”

“…Currently, some microbes have been identified for having mannolytic activity such as Weissella viridescens LB37 [3], Gloeophyllum trabeum CBS900.73 [49], Neosartorya fischeri P1 [51], Arabidopsis thaliana [50], Bacillus nealsonii [10], Bacillus subtilis Bs5 [21], Bacillus subtilis NM-39 [30], Bacillus sp KKO1 [18], Thermotoga neopalitana 5068 [15]. Groups of fungi also exhibits mannolytic activity such as Streptomyces, Aspergillus and Trichoderma [52,27,10,14].…”

Prospects for Bio‐Industrial Application of an Extremely Alkaline Mannanase FromBacillus subtilissubsp.inaquosorumCSB31

“…The released reducing sugars were measured using the 3,5‐dinitrosalicyclic (DNS) method with LBG as the substrate against a standard curve of mannose . An appropriately diluted enzyme (100 µl) was mixed with 1% (w/v) LBG (100 µl) in 10 mM KCl/NaOH buffer (pH 12.5) and incubated at 60 °C for 30 min in a water bath, and then 100 µl DNS reagent was added and boiled for 10 min.…”

Isolasi Bakteri Pendegradasi Xilan dan Manan dari Perairan Indonesia

“…Tidak banyak penelitian yang melaporkan enzim mananase alkali dari bakteri laut, terutama dari perairan Indonesia. Mannanase alkali banyak dilaporkan diproduksi dari Bacillus (Hatada et al, 2005) dan Streptomyces (Yoo et al, 2015;Pradeep et al, 2016) (2014) mengisolasi Bacillus manolitik dari air laut di Pul au Pari, DKI Jakarta untuk produksi manooligosakarida. Potensi mananase alkali sangat luas, seperti dimanfaatkan oleh industri deterjen dan kertas karena sebagian besar proses di industri tersebut dilakukan di pH tinggi (Chauhan, Puri, Sharma, & Gupta, 2012;Dhawan & Kaur, 2007;Hatada et al, 2005).…”