A novel long noncoding RNA FAF inhibits apoptosis via upregulating FGF9 through PI3K/AKT signaling pathway in ischemia–hypoxia cardiomyocytes

Osteosarcoma (OS) is the most common malignant bone tumor in teens. Non-coding RNA activated by DNA damage (NORAD), a long non-coding RNA (lncRNA), has been reported to be involved in cancer biology, although its role in OS remains largely unknown. In the present study reverse transcription-quantitative PCR (RT-qPCR) was used to determine the expression levels of NORAD and miR-155-5p in samples from patients with OS. OS cell lines (Saos-2 and U2OS) were used as cell models. The biological influence of NORAD on OS cells was studied in vitro using Cell Counting Kit-8 and Transwell assays. The interaction between NORAD and miR-155-5p was clarified by bioinformatics analysis, RT-qPCR, luciferase reporter assay and RNA immunoprecipitation. NORAD was significantly increased in OS samples in comparison with controls, while miR-155-5p was reduced. Knockdown of NORAD and transfection of miR-155-5p mimics markedly inhibited the viability, migration and invasion of OS cells. There was a negative correlation between NORAD and miR-155-5p expression levels in OS samples. Taken together, the results of the present study indicated that the NORAD/miR-155-5p axis played a crucial role in regulating the proliferation, migration and invasion of OS cells. It is hypothesized that NORAD and miR-155-5p may serve as potential novel therapeutic targets for OS management.

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“…In recent years, studies have shown that lncRNAs play important roles in the progression of many diseases (24)(25)(26).…”

Section: Discussionmentioning

confidence: 99%

lncRNA NORAD promotes the progression of osteosarcoma via targeting of miR‑155‑5p

et al. 2021

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“…One novel lncRNA FAF (FGF9-associated factor),verified by bioinformatics 66 , upon its overexpression could significantly inhibit ischemia-induced cardiomyocytes apoptosis. Knockdown of FAF could induced apoptosis.…”

Section: Lncrna Malat1mentioning

confidence: 97%

Long Non-Coding RNAs: Crucial Players of Cardiomyocyte Apoptosis

Chen¹,

Liu²,

Li³

et al. 2019

J Cardiol and Cardiovasc Sciences

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Long non-coding RNAs (lncRNAs) have gained more attention in recent years as a potential new regulator of nearly all biological regulation. LncRNAs are over 200 nucleotides in length, and it can interact with other non-coding RNAs or specific proteins to influence the gene expression. Cardiomyocyte apoptosis is associated with cardiovascular diseases. Accumulating studies have uncovered novel lncRNAs-mediated regulation of cardiovascular diseases; however, the knowledge of the mechanisms by how to act is still limited. This review highlights the role of lncRNAs involved in cardiomyocyte apoptosis with a focus on the regulatory axis. These examples may provide helpful insights on how lncRNAs interfere with cardiomyocyte apoptosis.

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“…A specific analysis of lncRNAs involved in myocardial infarction has been performed by Chen and colleagues, which reports numerous studies aimed at performing high‐throughput screening of lncRNAs which are differentially expressed in various heart diseases 91 . They also report an implication for the lncRNAs ZFAS1, 92,93 HOTAIR, 94 MALAT1, 95,96 GAS5, 97 FAF, 98 TTTY15, 99 ECRAR, 100 AK080084, 101 NR_045363, 102 TUG1, 103 and Meg3 91,104 …”

Section: Lncrnas In Obesity‐associated Diseasesmentioning

confidence: 99%

Role of long non‐coding RNAs in adipogenesis: State of the art and implications in obesity and obesity‐associated diseases

et al. 2021

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Summary Obesity is an evolutionary, chronic, and relapsing disease that consists of a pathological accumulation of adipose tissue able to increase morbidity for high blood pressure, type 2 diabetes, metabolic syndrome, and obstructive sleep apnea in adults, children, and adolescents. Despite intense research over the last 20 years, obesity remains today a disease with a complex and multifactorial etiology. Recently, long non‐coding RNAs (lncRNAs) are emerging as interesting new regulators as different lncRNAs have been found to play a role in early and late phases of adipogenesis and to be implicated in obesity‐associated complications onset. In this review, we discuss the most recent advances on the role of lncRNAs in adipocyte biology and in obesity‐associated complications. Indeed, more and more researchers are focusing on investigating the underlying roles that these molecular modulators could play. Even if a significant number of evidence is correlation‐based, with lncRNAs being differentially expressed in a specific disease, recent works are now focused on deeply analyzing how lncRNAs can effectively modulate the disease pathogenesis onset and progression. LncRNAs possibly represent new molecular markers useful in the future for both the early diagnosis and a prompt clinical management of patients with obesity.

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A novel long noncoding RNA FAF inhibits apoptosis via upregulating FGF9 through PI3K/AKT signaling pathway in ischemia–hypoxia cardiomyocytes

Cited by 28 publications

References 37 publications

lncRNA NORAD promotes the progression of osteosarcoma via targeting of miR‑155‑5p

lncRNA NORAD promotes the progression of osteosarcoma via targeting of miR‑155‑5p

Long Non-Coding RNAs: Crucial Players of Cardiomyocyte Apoptosis

Role of long non‐coding RNAs in adipogenesis: State of the art and implications in obesity and obesity‐associated diseases

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