A Novel Liquid Mixture in Iron Chemical-Biological Process Using Immobilized <i>Acidithiobacillus ferrooxidans</i>

Abstract

Abstract. Iron chemical-biological process was widely applied in desulfurization and hydrometally. 9K medium is used usually in this process. However, 9K medium is regarded as an optimal medium for suspended A. ferrooxidans culture. Iron oxidation rate is slow and some precipitation would be formed when the medium is used in this process. In this paper, a novel liquid mixture for iron chemical-biological process was reported: (NH 4 ) 2 SO 4 1 g/L, K 2 HPO 4 0.2 g/L, KCl 0.15, iron 17 g/L with initial pH 1.8. A verification test indicated that Fe 2+ oxidation rate was 6.1 g/(L·h), which was better than the results obtained in previous experiments. At the same time the precipitation formed is very small compared with all the experiments. So the liquid mixture may apply in this chemical-biological process better.

A Method for Preparation of Desulfurizing Biomass from Crude Oil

“…This colony could contain some bacteria such as multi strains of Acidithioballus which have the ability to desulfurize or defrade the crude oil and its derivative [14,15]. But mainly, 9k medium encourages the growth of the strains of thiobacillus [16,17] in addition to preferential tendency to medium PTCC 105 [11,18]. It was founded that 9k medium can extract four stains of Acidithiobacillus ferrooxidans which have been identified by phenotypicand 16S rDNA sequence analyses as shown in Figure 3.…”