A novel lipoprotein-mimic nanocarrier composed of the modified protein and lipid for tumor cell targeting delivery

The mechanism of enhancement on oral absorption of paclitaxel by N-octyl-O-sulfate chitosan micelles

“…The cell suspension was obtained in lysis buffer by blowing air repeatedly and tenderly. After centrifugation at 12,000 rpm for 5 min, 20 mL of supernatant was used for BCA protein assay [25] to quantitate the cells protein. In addition, 100 mL of supernatant was mixed with equal-volume of methyl cyanides for protein precipitation, vortexed for 5 min, and centrifuged at 12,000 rpm for 10 min.…”

Long-circulating lipoprotein-mimic nanoparticles for smart intravenous delivery of a practically-insoluble antineoplastic drug: Development, preliminary safety evaluations and preclinical pharmacokinetic studies

“…Recent reports elucidated that the hydrophobic interactions of albumin with lipophilic drugs and/or fatty acid chains of phospholipids could provide potential stabilization of many 85 drug-loaded phospholipid-enriched NPs (Xu et al, 2010;Zhou et al, 2013;McMahon et al, 2015;Gao et al, 2013). The reactivity of albumin with many targeting legends, via the amino and/or carboxylic groups, can minimize the possible association with serum proteins and/or rapid clearance of the reticuloendothelial system (Xu et al, 2010;Zhou et al, 2013).…”

“…The reactivity of albumin with many targeting legends, via the amino and/or carboxylic groups, can minimize the possible association with serum proteins and/or rapid clearance of the reticuloendothelial system (Xu et al, 2010;Zhou et al, 2013).…”

Tumor targeting effects of a novel modified paclitaxel-loaded discoidal mimic high density lipoproteins

“…The cytotoxicities of P-d-rHDL and cP-d-rHDL with or without LCAT were determined through the MCF-7 cells viability using MTT assays (Xu et al, 2010). The cells were seeded in 96-well plates at a density of 10 5 cells/well in their media for 24 h. Then the medium was washed with PBS (pH 7.4) twice, and then incubated with samples diluted in DMEM at 37 C for 6 h. 100 mL of MTT…”