A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603

Batrakov, S. G.; Rodionova, T. A.; Esipov, S. E.; Polyakov, N. B.; Шейченко, В. И.; Shekhovtsova, Nina V.; Lukin, S. M.; Panikov, N. S.; Nikolaev, Yu. A.

doi:10.1016/j.bbalip.2003.09.004

Cited by 43 publications

(23 citation statements)

References 31 publications

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“…3B and C). The cleavage mode of amino acid residues indicated the existence of a cyclic structure (27). MS/MS data also showed that compound 2 contained the same amino acid sequence as did compound 1 ( Fig.…”

Section: Resultsmentioning

confidence: 79%

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Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp. Strain 176 Suppress the Motility of Vibrio alginolyticus

Xiu

Zhang

et al. 2017

Appl Environ Microbiol

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Bacterial motility is a crucial factor during the invasion and colonization processes of pathogens, which makes it an attractive therapeutic drug target. Here, we isolated a marine bacterium (Vibrio alginolyticus strain 178) from a seamount in the tropical West Pacific that exhibits vigorous motility on agar plates and severe pathogenicity to zebrafish. We found that V. alginolyticus 178 motility was significantly suppressed by another marine bacterium, Bacillus sp. strain 176, isolated from the same niche. We isolated, purified, and characterized two different cyclic lipopeptides (CLPs) from Bacillus sp. 176 using high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopy. The two related CLPs have a pumilacidin-like structure and were both effective inhibitors of V. alginolyticus 178 motility. The CLPs differ by only one methylene group in their fatty acid chains. In addition to motility suppression, the CLPs also induced cell aggregation in the medium and reduced adherence of V. alginolyticus 178 to glass substrates. Notably, upon CLP treatment, the expression levels of two V. alginolyticus flagellar assembly genes (flgA and flgP) dropped dramatically. Moreover, the CLPs inhibited biofilm formation in several other strains of pathogenic bacteria without inducing cell death. This study indicates that CLPs from Bacillus sp. 176 show promise as antimicrobial lead compounds targeting bacterial motility and biofilm formation with a low potential for eliciting antibiotic resistance.IMPORTANCE Pathogenic bacteria often require motility to establish infections and subsequently spread within host organisms. Thus, motility is an attractive therapeutic target for the development of novel antibiotics. We found that cyclic lipopeptides (CLPs) produced by marine bacterium Bacillus sp. strain 176 dramatically suppress the motility of the pathogenic bacterium Vibrio alginolyticus strain 178, reduce biofilm formation, and promote cellular aggregation without inducing cell death. These findings suggest that CLPs hold great promise as potential drug candidates targeting bacterial motility and biofilm formation with a low overall potential for triggering antibiotic resistance.KEYWORDS lipopeptide, pumilacidin, Vibrio alginolyticus, motility, antibiotic, antimicrobial, antibiofilm B acterial motility is one of several crucial factors during the initial infection and colonization processes of pathogens. In particular, it helps pathogens overcome the repulsive forces between the bacterial cell wall and the host tissues and facilitates host attachment (1-3). The flagellum is one of the main locomotive organelles respon-

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Section: Resultsmentioning

confidence: 79%

“…Fractions of each eluted peak were tested for antimotility activity as described above. The active fractions were passed through the HPLC column two times to assess stability (27).…”

Section: Methodsmentioning

confidence: 99%

Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp. Strain 176 Suppress the Motility of Vibrio alginolyticus

Xiu

Zhang

et al. 2017

Appl Environ Microbiol

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“…Clone bo22C02 showed a close relationship with B. sporothermodurans type strain, which is able to produce highly heat-resistant endospores (Pettersson et al 1996), and with two others species, Bacillus sonorensis and Bacillus licheniformis, the latter being both thermophilic and halotolerant (Batrakov et al 2003).…”

Section: Discussionmentioning

confidence: 96%

Analysis of the composition of bacterial communities in oil reservoirs from a southern offshore Brazilian basin

Sette

Simioni

Vasconcellos

et al. 2006

Antonie van Leeuwenhoek

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The aim of this study was to characterize and compare the bacterial community structure of two distinct oil samples from a petroleum field in Brazil by using both molecular, based on the construction of 16S rRNA gene libraries, and cultivation methods. Statistical comparisons of libraries based on Amplified Ribosomal DNA Restriction Analysis (ARDRA) data revealed no significant differences between the communities recovered in the non-biodegraded (NBD) and highly biodegraded oils (HBD). BlastN analysis of the 16S rRNA gene sequences representative of distinct ribotypes from both oils showed the presence of nine different bacterial genera in these samples, encompassing members of the genera Arcobacter, Halanaerobium, Marinobacter, Propionibacterium, Streptomyces, Leuconostoc, Acinetobacter, Bacillus and Streptococcus. Enrichments obtained using oil as inoculum and sole carbon source yielded bacterial isolates showing high 16S rRNA gene sequence similarity with Achromobacter xylosoxidans, Bacillus subtilis, Brevibacillus sp., Dietzia sp. and Methylobacterium sp. Comparison between the data obtained using cultivation-independent and enrichment cultures suggests that different selection of community members may occur when using distinct approaches. All the organisms found, except for Leuconostoc sp. and Streptococus sp., have been previously reported in the literature as hydrocarbon degraders and/or associated to oil field environments.

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“…33) An isolate from a deep oil well grew in a medium within a range of 0 to 13% NaCl. 34) Other isolates from oil field injection water 35) and subsurface thermal water 36) grew in media at pH 9.0 and 9.8 respectively. We observed that the B. licheniformis isolates grew well in a range from pH 9.0 to 10.0 (Fig.…”

Section: Discussionmentioning

confidence: 99%

BacillusSpecies Predominated in an Incineration Ash Layer at a Landfill

Mizuno

Fukuda

Fujii

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603

Cited by 43 publications

References 31 publications

Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp. Strain 176 Suppress the Motility of Vibrio alginolyticus

Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp. Strain 176 Suppress the Motility of Vibrio alginolyticus

Analysis of the composition of bacterial communities in oil reservoirs from a southern offshore Brazilian basin

BacillusSpecies Predominated in an Incineration Ash Layer at a Landfill

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