A novel lineage of candidate pheromone receptors for sex communication in moths

Bastin-Héline, Lucie; Fouchier, Arthur de; Cao, Song; Koutroumpa, Fotini; Caballero-Vidal, Gabriela; Robakiewicz, Stefania; Monsempes, Christelle; François, Marie-Christine; Ribeyre, Tatiana; Maria, Annick; Chertemps, Thomas; Cian, Anne De; Walker, William B.; Wang, Guirong; Jacquin‐Joly, Emmanuelle; Montagné, Nicolas

doi:10.7554/elife.49826

Cited by 59 publications

(60 citation statements)

References 55 publications

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“…However, according to the FPKM values, HrhoOR14 exhibited male-biased expression while HrhoOR30 exhibited female-biased expression. This expression pattern was similar to the novel lineage of PRs, whereby approximately one-half of the ORs were male-biased and the other half were female-biased (Bastin-Héline et al, 2019).…”

Section: Discussionsupporting

confidence: 62%

“…However, two ORs (HrhoOR14 and 30) clustered into a novel lineage distantly related to the conserved lepidopteran PR clade. Notably, several DpunPRs (Shen et al, 2020) and SlitOR5 (Bastin-Héline et al, 2019) that grouped with HrhoOR14 and 30 were recently characterized as a new evolution origin of PRs in Lepidoptera. We speculate that these two ORs may be involved in the detection of H. rhodope sex pheromones, although further functional analysis is needed to confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, H. rhodope ORs lacked in the classical moth pheromone receptor (PR) clade, while HrhhoOR14 and HrhoOR30 clustered within a novel PR clade containing SlitOR5 in S. littoralis and several candidate PRs from D. punctatus. These ORs were recently identified as a novel lineage of PRs detecting type I pheromones in Lepidoptera (Bastin-Héline et al, 2019;Shen et al, 2020).…”

Section: Identification and Phylogenetic Analyses Of Ors In H Rhodopementioning

confidence: 99%

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Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Yang¹,

Dong²,

Sun³

et al. 2020

PeerJ

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Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), nine ionotropic receptors (IRs), eight gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR co-receptor (ORco), ionotropic receptors co-receptor, CO2 receptors, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, six ORs, five IRs and two GRs displayed female-biased expression, while two ORs displayed male-biased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Identification and Phylogenetic Analyses Of Ors In H Rhodopementioning

confidence: 99%

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Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Yang¹,

Dong²,

Sun³

et al. 2020

PeerJ

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“…However, we noticed that the two other minor pheromone components (Z11-16:OAc and Z7-12:OAc) failed to activate any of the six ORs tested, suggesting that some other ORs outside the PR clade are responsible for their detection. Recently, it was reported that in S. littoralis, SlittOR5, a member of the general odorant receptor clade, responds to the sex pheromone component Z9,E11-14:OAc, rather than one of the receptors in the PR clade [62].…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)

Guo

Liu

et al. 2020

Insects

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Pheromone receptors (PRs) found in the antennae of male moths play a vital role in the recognition of sex pheromones released by females. The fall armyworm (FAW), Spodoptera frugiperda, is a notorious invasive pest, but its PRs have not been reported. In this report, six candidate PRs (SfruOR6,11,13,16, 56 and 62) suggested by phylogenetic analysis were cloned, and their tissue-sex expression profiles were determined by quantitative real-time PCR (qPCR). All six genes except for SfruOR6 were highly and specifically expressed in the antennae, with SfruOR6, 13 and 62 being male-specific, while the other three (SfruOR11, 16 and 56) were male biased, suggesting their roles in sex pheromone perception. A functional analysis by the Xenopus oocyte system further demonstrated that SfruOR13 was highly sensitive to the major sex pheromone component Z9-14:OAc and the pheromone analog Z9,E12-14:OAc, but less sensitive to the minor pheromone component Z9-12:OAc; SfruOR16 responded weakly to pheromone component Z9-14:OAc, but strongly to pheromone analog Z9-14:OH; the other four candidate PRs did not respond to any of the four pheromone components and four pheromone analogs. This study contributes to clarifying the pheromone perception in the FAW, and provides potential gene targets for developing OR-based pest control techniques.

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“…Interestingly, H. rhodope ORs lacked in the classical moth pheromone receptor (PR) clade, while HrhhoOR14 and HrhoOR30 clustered within a novel PR clade containing SlitOR5 in S. littoralis and several candidate PRs from D. punctatus. These ORs were recently identified as a novel lineage of PRs detecting type Ⅰ pheromones in Lepidoptera(Bastin-Héline et al, 2019;Shen et al, 2020).…”

mentioning

confidence: 99%

Peer Review #1 of "Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae) (v0.1)"

Montagné¹

2020

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Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), 9 ionotropic receptor s (IRs), 8 gustatory receptors (GRs) and 2 sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR coreceptor (ORco), ionotropic receptor co-receptor, CO 2 receptor, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, 6 ORs, 5 IRs and 2 GRs displayed female-biased expression, while 2 ORs displayed malebiased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

show abstract

A novel lineage of candidate pheromone receptors for sex communication in moths

Cited by 59 publications

References 55 publications

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)

Peer Review #1 of "Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae) (v0.1)"

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