A novel lignin degradation bacterial consortium for efficient pulping

Wang, Yanxia; Liu, Quan; Yan, Lei; Gao, Yamei; Wang, Yanjie; Wang, Weidong

doi:10.1016/j.biortech.2013.04.033

Cited by 113 publications

(49 citation statements)

References 31 publications

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“…They identified Burkholderiales, Caulobacterales, Rhizobiales, Sphingobacteriales and Xanthomonadales as the active bacterial groups. Enterobacteriales and Pseudomonadales were also enriched in insect herbivore microbiomes and lignin-enriched cultures, respectively [39,40]. Interestingly, in our RWS consortia the abundance of Enterobacteriales increased after T1, but the number of OTUs decreased, suggesting the selection of specific strains, for example, K. variicola , within this group.…”

Section: Discussionmentioning

confidence: 90%

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

Jiménez

Dini‐Andreote

Elsas

2014

Biotechnol Biofuels

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BackgroundMixed microbial cultures, in which bacteria and fungi interact, have been proposed as an efficient way to deconstruct plant waste. The characterization of specific microbial consortia could be the starting point for novel biotechnological applications related to the efficient conversion of lignocellulose to cello-oligosaccharides, plastics and/or biofuels. Here, the diversity, composition and predicted functional profiles of novel bacterial-fungal consortia are reported, on the basis of replicated aerobic wheat straw enrichment cultures.ResultsIn order to set up biodegradative microcosms, microbial communities were retrieved from a forest soil and introduced into a mineral salt medium containing 1% of (un)treated wheat straw. Following each incubation step, sequential transfers were carried out using 1 to 1,000 dilutions. The microbial source next to three sequential batch cultures (transfers 1, 3 and 10) were analyzed by bacterial 16S rRNA gene and fungal ITS1 pyrosequencing. Faith’s phylogenetic diversity values became progressively smaller from the inoculum to the sequential batch cultures. Moreover, increases in the relative abundances of Enterobacteriales, Pseudomonadales, Flavobacteriales and Sphingobacteriales were noted along the enrichment process. Operational taxonomic units affiliated with Acinetobacter johnsonii, Pseudomonas putida and Sphingobacterium faecium were abundant and the underlying strains were successfully isolated. Interestingly, Klebsiella variicola (OTU1062) was found to dominate in both consortia, whereas K. variicola-affiliated strains retrieved from untreated wheat straw consortia showed endoglucanase/xylanase activities. Among the fungal players with high biotechnological relevance, we recovered members of the genera Penicillium, Acremonium, Coniochaeta and Trichosporon. Remarkably, the presence of peroxidases, alpha-L-fucosidases, beta-xylosidases, beta-mannases and beta-glucosidases, involved in lignocellulose degradation, was indicated by predictive bacterial metagenome reconstruction. Reassuringly, tests for specific (hemi)cellulolytic enzymatic activities, performed on the consortial secretomes, confirmed the presence of such gene functions.ConclusionIn an in-depth characterization of two wheat straw degrading microbial consortia, we revealed the enrichment and selection of specific bacterial and fungal taxa that were presumably involved in (hemi) cellulose degradation. Interestingly, the microbial community composition was strongly influenced by the wheat straw pretreatment. Finally, the functional bacterial-metagenome prediction and the evaluation of enzymatic activities (at the consortial secretomes) revealed the presence and enrichment of proteins involved in the deconstruction of plant biomass.

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Section: Discussionmentioning

confidence: 90%

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

Jiménez

Dini‐Andreote

Elsas

2014

Biotechnol Biofuels

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“…strains with lignin degradation ability, Microbacterium species were found to be present in the gut of the wood-infesting termites [47]. There are also reports of strains of Microbacterium able to degrade polycyclic aromatic hydrocarbons [48–50]. …”

Section: Discussionmentioning

confidence: 99%

Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation

Puentes-Téllez

Salles

2018

Microb Ecol

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Enriched microbial communities, obtained from environmental samples through selective processes, can effectively contribute to lignocellulose degradation. Unfortunately, fully controlled industrial degradation processes are difficult to reach given the intrinsically dynamic nature and complexity of the microbial communities, composed of a large number of culturable and unculturable species. The use of less complex but equally effective microbial consortia could improve their applications by allowing for more controlled industrial processes. Here, we combined ecological theory and enrichment principles to develop an effective lignocellulose-degrading minimal active microbial Consortia (MAMC). Following an enrichment of soil bacteria capable of degrading lignocellulose material from sugarcane origin, we applied a reductive-screening approach based on molecular phenotyping, identification, and metabolic characterization to obtain a selection of 18 lignocellulose-degrading strains representing four metabolic functional groups. We then generated 65 compositional replicates of MAMC containing five species each, which vary in the number of functional groups, metabolic potential, and degradation capacity. The characterization of the MAMC according to their degradation capacities and functional diversity measurements revealed that functional diversity positively correlated with the degradation of the most complex lignocellulosic fraction (lignin), indicating the importance of metabolic complementarity, whereas cellulose and hemicellulose degradation were either negatively or not affected by functional diversity. The screening method described here successfully led to the selection of effective MAMC, whose degradation potential reached up 96.5% of the degradation rates when all 18 species were present. A total of seven assembled synthetic communities were identified as the most effective MAMC. A consortium containing Stenotrophomonas maltophilia, Paenibacillus sp., Microbacterium sp., Chryseobacterium taiwanense, and Brevundimonas sp. was found to be the most effective degrading synthetic community.Electronic supplementary materialThe online version of this article (10.1007/s00248-017-1141-5) contains supplementary material, which is available to authorized users.

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“…Mature-phase compost was a preferred niche for Chloroflexi (de Gannes et al, 2013) and they have been previously described as soil oligotrophs (Fierer et al, 2007), so application of maize residue will reduce their relative abundance. Strains belonging to the order Clostridiales have been found in a lignin degradation bacterial consortium (Wang et al, 2013), so application of easily decomposable organic material might reduce their relative abundance.…”

Section: Effect Of Residue Application On the Relative Abundance Of Bmentioning

confidence: 99%

Bacterial community structure in maize residue amended soil with contrasting management practices

Ramírez-Villanueva

Bello–López

Navarro‐Noya

et al. 2015

Applied Soil Ecology

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A novel lignin degradation bacterial consortium for efficient pulping

Cited by 113 publications

References 31 publications

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia

Construction of Effective Minimal Active Microbial Consortia for Lignocellulose Degradation

Bacterial community structure in maize residue amended soil with contrasting management practices

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