A novel lectin-based enzyme-linked immunosorbent assay for the measurement of IgA1 in serum and secretory IgA1 in secretions

Airway glycoprotein secretion parallels production and predicts airway obstruction in pulmonary allergy☆

“…Jacalin binds to many glycoproteins possessing O-linked oligosaccharides 16,17 and has previously been shown to bind IgA, a property that proved useful in devising immunoassays for detection of this glycosylated Ig from bodily fluids. 18 Our data indicate that the assays provide comparable measurements of total airway glycoproteins that correlate with both glycoprotein production and airway hyperresponsiveness, commonly used indexes of airway obstruction. These assays should prove useful in investigating the biology of airway glycoprotein secretion further under diverse conditions.…”

Protective Immunity in Human Filariasis: A Role for Parasite‐Specific IgA Responses

“…A lectin-based method was used to quantify total IgA1 in serum samples, as described elsewhere [18]. First, 96-well polystyrene plates (MaxiSorp; Nunc) were coated with Jacalin lectin (Artocarpus integrifolia) (L-3515; Sigma) at a concentration of 10 g/well in 0.15 mol/L carbonate buffer (pH 9.6).…”

High‐Performance Liquid Chromatography of Biological Macromolecules

“…426 Jacalin, extracted from seeds of the jack fruit (Artocarpus integrifolia), has a galactose-specific interaction, 427 providing an affinity for proteins with O-linked oligosaccharides. 428 A useful immunochemical feature is its ability to isolate IgA, 429 which has been used to deduce the structure of the IgA core oligosaccharide by enzymatic scission and MS. 430 A micropreparative isolation technique for jacalin based on weak anion exchange chromatography has been published. 431 A wide spectrum of additional lectins have been purified from various sources, showing a substantial differences in their oligosaccharide binding patterns.…”