A novel laminar-flow-based bioaerosol test system to determine biological sampling efficiencies of bioaerosol samplers

Pogner, Clara; Konlechner, Anja; Unterwurzacher, Verena; Kolk, Annette; Hinker, Manfred; Mölter, Leander; Strauss, Joseph; Gorfer, Markus; Strauss-Goller, Sabine

doi:10.1080/02786826.2018.1562151

Cited by 5 publications

(6 citation statements)

References 62 publications

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“…The newly developed filter system was tested under controlled conditions in a bioaerosol test chamber (Pogner et al 2019) and during a field study with different indoor mold situations. In both cases the MAS-100 NT impactor was used as a reference device for testing the suitability of the novel filter holders for bioaerosol sampling.…”

Section: Air Samplingmentioning

confidence: 99%

“…The sampling conditions in the bioaerosols chamber and the according experimental setup used for this study are based on Pogner et al 2019. In short, the test system that was used for the biological validation of the sampling device, comprised of the CCB 3000 bioaerosol chamber (Calibration Chamber for Bioaerosols 3000, Palas, Germany) and an LSA nebulizer (Liquid Sparging Aerosolizer, CH Technologies, United States).…”

Section: Bioaerosol Chambermentioning

confidence: 99%

“…For the spore suspensions, fungal strains were cultivated on malt extract agar (Merck, United States) for 2-3 weeks at room temperature (22 °C). Trichoderma longibrachiatum was used as test organism, since it was used to validate the CCB 3000 bioaerosol chamber as described in Pogner et al 2019 and its conidia can be aerosolized without detergent. As described previously, the use of detergents in aerosolization creates a high load of unspecific particle background and thus disqualifies parallel control measurements with the particle counter installed inside the chamber.…”

Section: Bioaerosol Chambermentioning

confidence: 99%

“…A bioaerosol chamber was used to establish stable sampling conditions for the reference sampler (MAS) and the air filtration-based system to be validated (VAC) as described before (Pogner et al 2019). Three different filter materials were tested with the air filtration-based sampler in this comparative study under controlled conditions.…”

Section: Sampling Device Comparison Under Controlled Conditionsmentioning

confidence: 99%

“…Specific technical limitations of each sampling device might amplify these differences. To compensate for such uncertainties, various bioaerosol chambers have been implemented to enable stabilized sampling condition (Dybwad et al 2014;Estill et al 2008;Feather and Chen 2003;Kesavan and Sagripanti 2015;Pogner et al 2019;Simon and Duquenne 2013;Wang et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Simple Bioaerosol Collection Filter System Using a Conventional Vacuum Cleaner for Sampling

Unterwurzacher

Bruck²,

Biedermann

et al. 2021

Aerosol Sci Eng

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Although numerous bioaerosol samplers for counting and identifying airborne microorganisms are available, the considerably high purchase and maintenance costs for the sampler often prevent broad monitoring campaigns for occupational or environmental surveillance of bioaerosols. We present here a newly developed simple adapter and filter system (TOP filter system) designed to collect bioaerosol particles from a defined air volume using conventional vacuum cleaners as air pumps. We characterized the physical properties of the system using air flow measurements and validated the biological performance. The culture-based detection capacities for airborne fungal species were compared to a standard impaction sampler (MAS-100 NT) under controlled conditions in a bioaerosol chamber (using Trichoderma spores as the test organism) as well as in the field. In the chamber, an overall equivalent detection capacity between all tested filters was recorded, although a significant underrepresentation of the TOP filter system for Trichoderma spores were seen in comparison to the MAS-100 NT. In a comparative field study (n = 345), the system showed similar biological sampling efficiencies compared to the MAS-100 NT impactor, only the diversity of identified fungal communities was slightly lower on the filters. Thus, the system is suitable for large-scale environmental sampling operations where many samples have to be taken in parallel at a given time at distant locations. This system would allow endeavors such as antibiotics resistance monitoring or hygiene surveys in agricultural or occupational settings.

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Section: Air Samplingmentioning

confidence: 99%

Section: Bioaerosol Chambermentioning

confidence: 99%

Section: Bioaerosol Chambermentioning

confidence: 99%

Section: Sampling Device Comparison Under Controlled Conditionsmentioning

confidence: 99%