A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H<sub>2</sub>O<sub>2</sub> system

Yang, Fengzhen; Li, Jiansen; Dong, Haowei; Wang, Guanjie; Han, Jingti; Xu, Rui; Kong, Qianqian; Huang, Jingcheng; Xiang, Yaodong; Yang, Qingqing; Sun, Xia; Guo, Yemin

doi:10.1039/d1an02060a

Cited by 13 publications

(6 citation statements)

References 47 publications

(49 reference statements)

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“…The aptamer structure is unstable in the binding buffer without Mg 2+ ( Zhao et al, 2020 ), and Mg 2+ is important for stabilizing the two- and three-dimensional structures of aptamers ( Huang et al, 2013 ). In addition to Mg 2+ , the Na + , K + and pH value of a buffer system also have an effect on the binding of aptamers to targets, and the optimization of them can significantly improve the efficiency of aptamer sensors in detecting targets ( Hianik et al, 2007 ; Belleperche and DeRosa, 2018 ) and significantly increase the sensitivity of aptamer sensors ( Yang et al, 2022 ). The binding of thrombin to its aptamer also depends on pH and electrolytes ( Hianik et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation

Zhang

et al. 2023

Front. Chem.

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Introduction: Aptamers are valuable for bioassays, but aptamer-target binding is susceptible to reaction conditions. In this study, we combined thermofluorimetric analysis (TFA) and molecular dynamics (MD) simulations to optimize aptamer-target binding, explore underlying mechanisms and select preferred aptamer.Methods: Alpha-fetoprotein (AFP) aptamer AP273 (as the model) was incubated with AFP under various experimental conditions, and melting curves were measured in a real-time PCR system to select the optimal binding conditions. The intermolecular interactions of AP273-AFP were analysed by MD simulations with these conditions to reveal the underlying mechanisms. A comparative study between AP273 and control aptamer AP-L3-4 was performed to validate the value of combined TFA and MD simulation in selecting preferred aptamers.Results: The optimal aptamer concentration and buffer system were easily determined from the dF/dT peak characteristics and the melting temperature (Tm) values on the melting curves of related TFA experiments, respectively. A high Tm value was found in TFA experiments performed in buffer systems with low metal ion strength. The molecular docking and MD simulation analyses revealed the underlying mechanisms of the TFA results, i.e., the binding force and stability of AP273 to AFP were affected by the number of binding sites, frequency and distance of hydrogen bonds, and binding free energies; these factors varied in different buffer and metal ion conditions. The comparative study showed that AP273 was superior to the homologous aptamer AP-L3-4.Conclusion: Combining TFA and MD simulation is efficient for optimizing the reaction conditions, exploring underlying mechanisms, and selecting aptamers in aptamer-target bioassays.

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Section: Discussionmentioning

confidence: 99%

Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation

Zhang

et al. 2023

Front. Chem.

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“…At present, the H 2 O 2 molecule detection method is used in the design of biosensors for glucose, xanthine, cholesterol, organophosphorus and others. [19][20][21] Among them, the detection of glucose is considered to be of great significance in clinical diagnosis and the food industry. The detection methods for glucose include colorimetry, photoelectrochemical technology, fluorescence, electrochemical analysis, etc.…”

Section: Glucose Sensing Applicationmentioning

confidence: 99%

Copper-based biological alloys and nanocomposites for enzymatic catalysis and sensing applications

Chen

Yang

2023

Nanoscale

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“…Recently, Yang and co-workers developed an Au-tetrahedral DNA nanostructure (Au-TDN) and AgNPs that can be efficiently employed as an electrochemiluminescence aptasensor to determine the organophosphate pesticides . Additionally, the tetrahedron has separate probes at each vertex, which could boost the specific binding with OPs.…”

Section: Exploring Various Photochemical Processes For Pesticide Dete...mentioning

confidence: 99%

Exploring Various Photochemical Processes in Optical Sensing of Pesticides by Luminescent Nanomaterials: A Concise Discussion on Challenges and Recent Advancements

Paul,

Daga,

Dey

2023

ACS Omega

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Food safety is a burning global issue in this present era. The prevalence of harmful food additives and contaminants in everyday food is a significant cause for concern as they can adversely affect human health. More particularly, among the different food contaminants, the use of excessive pesticides in agricultural products is severely hazardous. So, the optical detection of residual pesticides is an effective strategy to counter the hazardous effect and ensure food safety. In this perspective, nanomaterials have played a leading role in defending the open threat against food safety instigated by the reckless use of pesticides. Now, nanomaterial-based optical detection of pesticides has reached full pace and needs an inclusive discussion. This Review covers the advancement of photoprocess-based optical detection of pesticides categorically using nanomaterials. Here, we have thoroughly dissected the photoprocesses (aggregation and aggregation-induced emission (AIE), charge transfer and intramolecular charge transfer (ICT), electron transfer and photoinduced electron transfer (PET), fluorescence resonance energy transfer (FRET), hydrogen bonding, and inner filter effect) and categorically demarcated their significant role in the optical detection of pesticides by luminescent nanomaterials over the last few years.

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A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H₂O₂ system

Abstract: In this work, a new type of Au-Tetrahedral DNA nanostructure (Au-TDN) was originally proposed and successfully applied in an electrochemiluminescence aptasensor to detect organophosphorus pesticides (Ops). The aptamers modified by...

Cited by 13 publications

References 47 publications

Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation

Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation

Copper-based biological alloys and nanocomposites for enzymatic catalysis and sensing applications

Exploring Various Photochemical Processes in Optical Sensing of Pesticides by Luminescent Nanomaterials: A Concise Discussion on Challenges and Recent Advancements

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A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H2O2 system

Abstract: In this work, a new type of Au-Tetrahedral DNA nanostructure (Au-TDN) was originally proposed and successfully applied in an electrochemiluminescence aptasensor to detect organophosphorus pesticides (Ops). The aptamers modified by...

Cited by 13 publications

References 47 publications

Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation

Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation

Copper-based biological alloys and nanocomposites for enzymatic catalysis and sensing applications

Exploring Various Photochemical Processes in Optical Sensing of Pesticides by Luminescent Nanomaterials: A Concise Discussion on Challenges and Recent Advancements

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A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol–H₂O₂ system