A novel L-type lectin was required for the multiplication of WSSV in red swamp crayfish (Procambarus clakii)

Abstract

L-type lectins are involved in glycoproteins secretory pathways and are associated with many immune responses. There is growing evidence that L-type lectins are also involved in viral replication. In this study, a novel L-type lectin (named as PcL-lectin) was identified from red swamp crayfish (Procambarus clakii). Gene sequencing and phylogenetic tree analysis results showed that the PcL-lectin was a kind of endoplasmic reticulum Golgi intermediate compartment-53 (ERGIC-53). The expression level of PcL-lectin was significantly down regulated in crayfish after challenged with white spot syndrome virus (WSSV). Recombinant PcL-lectin protein facilitated the replication of WSSV in crayfish. In addition, WSSV replication was decreased when endogenous PcL-lectin was knocked down by RNA interference in crayfish. Furthermore, PcL-lectin may interact with VP24, an envelope protein of WSSV. Our results suggest that PcL-lectin may be required for the multiplication of WSSV, and will pave a new way for the developing of strategies against WSSV infection.

Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus)

“…ERGIC-53 is a target of the ATF6 pathway of UPR [42]. PcL-lectin, a homolog of ERGIC-53, may interact with VP24 and is required for the multiplication of WSSV [12]. As a homolog of ERGIC-53, MjLTL2 may be a target of MjATF6 in M. japonicus and participate in WSSV proliferation.…”

“…Conversely, ERGIC-53 helps in the replication of infectious arenavirus, coronavirus, and filovirus particles [11]. A novel L-type lectin is required for the multiplication of white spot syndrome virus (WSSV) in red swamp crayfish Procambarus clakii [12].…”

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

“…from P. clarkii as L-type lectins (129), and PlFLP1 & 2 from Pacifastacus leniusculus as Ficolin-like lectins (130). Because of their role in activating the ProPO system, LGBP are the most studied type of PRRs and have been identified to belong to the typical b-1,3-glucanase related protein (BGRP) family that participates in the recognition of a wide range of pathogens, including bacteria and fungi like the A. astaci, the causative agent of the plague disease in crayfish [further details are found in (126)].…”

“…In fact, among the 13 animal classes of lectins identified (according to their structural characteristics), crayfish lectins were reported to belong to at least three structural groups. Pc-Lec2 & 3 from P. clarkii as C-type lectins ( 126 , 128 ), PcL-lectin from P. clarkii as L-type lectins ( 129 ), and PlFLP1 & 2 from Pacifastacus leniusculus as Ficolin-like lectins ( 130 ). Because of their role in activating the ProPO system, LGBP are the most studied type of PRRs and have been identified to belong to the typical β-1,3-glucanase related protein (BGRP) family that participates in the recognition of a wide range of pathogens, including bacteria and fungi like the A. astaci , the causative agent of the plague disease in crayfish [further details are found in ( 126 )].…”

Diet Comparison of Coexisting Collared Owlet (Glaucidium brodiei Burton, 1836), Spotted Owlet (Athene brama Temminck, 1821) and Eurasian Eagle Owl (Bubo bubo Linnaeus, 1758) in Wildlife Park Lohi Bher, Rawalpindi, Pakistan

“…With the capability to infect a wide range of crustaceans, including shrimp, crab and crayfish, WSSV is at present a major scourge to shrimp cultivation and has caused great economic loss in aquaculture industry worldwide since 1990s (Chou et al, 1995;Lo et al, 1996;Van Hulten et al, 2001). Up to date, there is lack of effective strategies against WSSV infection (Dai et al, 2016). The studies of WSSV replication and host-interactions would help to explore effective strategies for WSSV prevention and control.…”