A Novel Katanin-Tethering Machinery Accelerates Cytokinesis

Sasaki, Takema; Tsutsumi, Motosuke; Otomo, Kohei; Murata, Takashi; Yagi, Noriyoshi; Nakamura, Masayoshi; Nemoto, Tomomi; Oda, Y.

doi:10.1016/j.cub.2019.09.049

Cited by 26 publications

(33 citation statements)

References 49 publications

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“…Katanin recruitment during cell division does not require the Msd1-Wdr8 complex. Plant microtubules in mitotic arrays, spindles, and phragmoplasts, are also nucleated along preexisting microtubules in a γTuRCand augmin-dependent manner 26,27 , and katanin is required for the organization of mitotic microtubule arrays and for proper progression of mitosis and cytokinesis 21,22,28 . Subcellular localization of Msd1a-mCherry and GFP-KTN1 during mitosis revealed partially overlapping but distinct distributions.…”

Section: Resultsmentioning

confidence: 99%

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An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites

et al. 2021

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Microtubules are severed by katanin at distinct cellular locations to facilitate reorientation or amplification of dynamic microtubule arrays, but katanin targeting mechanisms are poorly understood. Here we show that a centrosomal microtubule-anchoring complex is used to recruit katanin in acentrosomal plant cells. The conserved protein complex of Msd1 (also known as SSX2IP) and Wdr8 is localized at microtubule nucleation sites along the microtubule lattice in interphase Arabidopsis cells. Katanin is recruited to these sites for efficient release of newly formed daughter microtubules. Our cell biological and genetic studies demonstrate that Msd1-Wdr8 acts as a specific katanin recruitment factor to cortical nucleation sites (but not to microtubule crossover sites) and stabilizes the association of daughter microtubule minus ends to their nucleation sites until they become severed by katanin. Molecular coupling of sequential anchoring and severing events by the evolutionarily conserved complex renders microtubule release under tight control of katanin activity.

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Section: Resultsmentioning

confidence: 99%

“…Therefore, Msd1-Wdr8 does not appear to play a major role for katanin recruitment on mitotic microtubules. A phragmoplast-localized plant-specific protein CORD4 contributes to tethering of katanin to the distal end of phragmoplast microtubules 28 .…”

Section: Resultsmentioning

confidence: 99%

An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites

et al. 2021

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“…Image analysis with two-photon excitation confocal microscopy with spinning disk unit was performed as previously described ( Otomo et al 2015 , Sasaki et al 2019 ). The GFP and chlorophyll signals were excited by 920-nm femtosecond light pulses generated by a mode-locked titanium-sapphire laser light source (Mai Tai eHP DeepSee; Spectra Physics).…”

Section: Methodsmentioning

confidence: 99%

Autophagy Contributes to the Quality Control of Leaf Mitochondria

Nakamura

Hagihara

Otomo

et al. 2020

Plant and Cell Physiology

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In autophagy, cytoplasmic components of eukaryotic cells are transported to lysosomes or the vacuole for degradation. Autophagy is involved in plant tolerance to the photooxidative stress caused by ultraviolet B (UVB) radiation, but its roles in plant adaptation to UVB damage have not been fully elucidated. Here, we characterized organellar behavior in UVB-damaged Arabidopsis (Arabidopsis thaliana) leaves and observed the occurrence of autophagic elimination of dysfunctional mitochondria, a process termed mitophagy. Notably, Arabidopsis plants blocked in autophagy displayed increased leaf chlorosis after a 1-h UVB exposure compared to wild-type plants. We visualized autophagosomes by labeling with a fluorescent protein-tagged autophagosome marker, AUTOPHAGY8 (ATG8), and found that a 1-h UVB treatment led to increased formation of autophagosomes and the active transport of mitochondria into the central vacuole. In atg mutant plants, the mitochondrial population increased in UVB-damaged leaves due to the cytoplasmic accumulation of fragmented, depolarized mitochondria. Furthermore, we observed that autophagy was involved in the removal of depolarized mitochondria when mitochondrial function was disrupted by mutation of the FRIENDLY gene, which is required for proper mitochondrial distribution. Therefore, autophagy of mitochondria functions in response to mitochondrion-specific dysfunction as well as UVB damage. Together, these results indicate that autophagy is centrally involved in mitochondrial quality control in Arabidopsis leaves.

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“…Strong katanin mutant alleles show defects in these processes resulting in cell division plane defects [45,46]. In addition, a line with a mutation in CORD4, a protein recruiting katanin to the phragmoplast, shows a delayed progression of the phragmoplast to the division site [47]. After the initial definition of the future division plane by the PPB orientation, the spatial information is retained by some of the above-mentioned proteins, but the precise mechanism by which the leading edge of the phragmoplast grows in the precise direction of the division site remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Plant cell divisions: variations from the shortest symmetric path

Serra

Robinson

2020

Biochemical Society Transactions

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In plants, the spatial arrangement of cells within tissues and organs is a direct consequence of the positioning of the new cell walls during cell division. Since the nineteenth century, scientists have proposed rules to explain the orientation of plant cell divisions. Most of these rules predict the new wall will follow the shortest path passing through the cell centroid halving the cell into two equal volumes. However, in some developmental contexts, divisions deviate significantly from this rule. In these situations, mechanical stress, hormonal signalling, or cell polarity have been described to influence the division path. Here we discuss the mechanism and subcellular structure required to define the cell division placement then we provide an overview of the situations where division deviates from the shortest symmetric path.

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A Novel Katanin-Tethering Machinery Accelerates Cytokinesis

Cited by 26 publications

References 49 publications

An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites

An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites

Autophagy Contributes to the Quality Control of Leaf Mitochondria

Plant cell divisions: variations from the shortest symmetric path

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