A novel JNK induces innate immune response by activating the expression of antimicrobial peptides in Chinese mitten crab Eriocheir sinensis

Huang, Ying; Nie, Ximei; Zhu, Zhenke; Zhang, Xing; Bing-zhi, LI; Ge, Jiachun; Ren, Qian

doi:10.1016/j.molimm.2021.07.011

Cited by 10 publications

(2 citation statements)

References 39 publications

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“…63 A recent study showed that a JNK and its isoforms identified in Eriocheir sinensis can over activate the expression of antibacterial peptides to participate in antibacterial immune defense. 64 In this test, the detection results of the expression of inflammatory cytokines and antimicrobial peptides are consistent with the above test results.…”

Section: Discussionsupporting

confidence: 86%

“…JNK signal in Drosophila model can participate in immune function defects by regulating the expression of various immune related genes (such as AMPs) to meet the challenge of pathogens 63 . A recent study showed that a JNK and its isoforms identified in Eriocheir sinensis can over activate the expression of antibacterial peptides to participate in antibacterial immune defense 64 . In this test, the detection results of the expression of inflammatory cytokines and antimicrobial peptides are consistent with the above test results.…”

Section: Discussionsupporting

confidence: 82%

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Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway

Sun

Liu

Zhu

et al. 2023

Environmental Toxicology

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Tetrabromobisphenol A (TBBPA) is a common environmental pollutant which has multi‐organ toxicity to mammals. Eucalyptol (EUC) has super antioxidant biological activity. However, in this experimental study, we probed into the mechanism of toxic of TBBPA exposure on Grass carp hepatocytes (L8824 cells) and the antagonistic impact of EUC on TBBPA. We treated L8824 cells with 8 μg/ml TBBPA and/or 20 μM EUC for 24 h in this test research. The experiment results suggested that TBBPA exposure induced elevated levels of reactive oxygen species (ROS), led to oxidative stress, decreased SOD and CAT activities, decreased GSH and T‐AOC contents, exacerbated MDA accumulation, activated ASK1/JNK signaling pathway, and further increased the contents of mitochondrial dependent apoptosis pathway related indicators (Cyt‐C, Bax, Caspase 9, Caspase 3), while Bcl‐2 expression decreased. In addition, TBBPA exposure induced increased expression of TNF‐α, IL‐6, IL‐1β, and decreased expression of IL‐2, IFN‐γ, Hepcidin, β‐defensin, LEAP2. The oxidative stress level, ASK1/JNK signal pathway expression level, apoptosis ratio and cellular immune function of cells exposed to EUC alone did not change significantly. Combined exposure of TBBPA and EUC significantly reduced the proportion of apoptosis and restored cellular immune function. Therefore, these results suggest that EUC can effectively antagonize TBBPA‐induced apoptosis and immune dysfunction of L8824 cells by regulating ROS/ASK1/JNK signaling pathway.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 82%