A novel iron-regulated metal transporter from plants identified by functional expression in yeast.

Eide, David J.; Broderius, Margaret; Fett, Janette Palma; Guerinot, Mary Lou

doi:10.1073/pnas.93.11.5624

Cited by 1,247 publications

(1,104 citation statements)

References 34 publications

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“…0 Fe means 0 M Fe plus 50 M ferrozine. Mean values § SD were determined for three to eight measurements tip and in the upper root zones, induced by low iron and down-regulated in Wt loss of function mutants (Eide et al 1996;Robinson et al 1999;Vert et al 2002;Connolly et al 2003;Colangelo and Guerinot 2004;Jakoby et al 2004). FIT was also mainly expressed in roots near the root tip and in the upper root zones, induced by low iron and its promoter was down regulated in Wt loss of function mutants (Colangelo and Guerinot 2004;Jakoby et al 2004;this work).…”

Section: Discussionmentioning

confidence: 99%

“…This iron mobilization system is strictly regulated. At low iron supply FRO2 and IRT1 genes are induced whereas upon suYcient iron supply the genes are down regulated (Eide et al 1996;Robinson et al 1999;Vert et al 2002). A central transcriptional regulator in this process in tomato is the basic helix-loop-helix (bHLH) factor FER; the A. thaliana ortholog is encoded by the gene FIT (= fer-like iron deWciency-induced transcription factor, previously known as FRU and FIT1, gene number At2 g28160; Bauer et al 2004;Colangelo and Guerinot 2004;Jakoby et al 2004;Yuan et al 2005; FIT was renamed by Bauer, Guerinot and Ling).…”

Section: Introductionmentioning

confidence: 99%

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Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Hong-yu

Klatte

Jakoby

et al. 2007

Planta

224

202

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Networks of transcription factors control physiological, developmental and environmental responses. Root iron acquisition responses are controlled by the essential bHLH protein FIT. Recently, two group Ib BHLH genes were reported to be iron deWciency-regulated. Here, we studied expression patterns of these two group Ib BHLH genes and of their two closest homologs to analyze whether their regulation would support a function in iron deWciency responses. We found that BHLH038, BHLH039, BHLH100 and BHLH101 (comprising a subgroup of BHLH Ib genes) were up regulated by iron deWciency in roots and leaves. Single insertion mutants had no visible phenotype and were capable of inducing root iron acquisition responses, presumably due to functional redundancy. SpeciWc metal treatments like nickel, high zinc or high copper resulted in induction of the four BHLH Ib genes whereas high iron, low copper and low zinc repressed gene expression. Induction of the four BHLH Ib genes was also found in multiple iron acquisition mutants including Wt. Ectopic activation of FIT did not suppress the four BHLH Ib genes. Split-root analyses using promoter-GUS lines showed that FIT and BHLH100 promoters were controlled by diVerent local and systemic signals involved in their regulation by iron. These results indicated that the four BHLH Ib genes were induced independently from FIT by conditions causing iron deWciency. Taken together, BHLH038, BHLH039, BHLH100 and BHLH101 function diVerently from FIT and may be involved in mediating a signal related to iron deWciencyinduced stress and/or internal iron homeostasis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Hong-yu

Klatte

Jakoby

et al. 2007

Planta

224

202

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“…During the past few years several metal transporters have been isolated from Arabidopsis: Zn transporters ZIP1, 3, 4 (Grotz et al, 1998), Fe transporter IRT1 (Eide et al, 1996), and Cu transporter COPT1 (Kampfenkel et al, 1995). Several transporters like ZIP1, ZIP3 and IRT1 are expressed in response to metal de®ciency.…”

Section: Study the Organism At High Or Low Metal Exposurementioning

confidence: 99%

“…Several transporters like ZIP1, ZIP3 and IRT1 are expressed in response to metal de®ciency. IRT1 may also play a role in the uptake of other metals, because Cd, Zn, Co and Mn inhibited Fe uptake of IRT1 (Eide et al, 1996). Changing the regulation of the expression of these transporters may modify the uptake of metals to the cells or organelles in a useful way.…”

Section: Study the Organism At High Or Low Metal Exposurementioning

confidence: 99%

Genetic engineering in the improvement of plants for phytoremediation of metal polluted soils

Kärenlampi¹,

Schat²,

Vangronsveld³

et al. 2000

Environmental Pollution

226

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“…However, the IRT1 protein is not specific for iron. Other metals such as Zn 2+ , Mn 2+ and Co 2+ can be transported into plants via the IRT1 protein in Arabidopsis (Eide et al, 1996;Korshunova et al, 1999;Connolly et al, 2002;Henriques et al, 2002;Varotto et al, 2002;Vert et al, 2002). Following transportation into root cells, iron is chelated by citrate or nicotianamine and distributed to the shoots (Stephan and Scholz, 1993;Briat et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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These authors contributed equally to this work. SUMMARYIron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T-DNA insertion mutant, yellow and sensitive to iron-deficiency 1 (yid1), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID1 encodes the Arabidopsis Mediator complex subunit MED16. We demonstrated that YID1/MED16 interacted with another subunit, MED25. MED25 played an important role in regulation of iron homeostasis by interacting with EIN3 and EIL1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT, IRT1 and FRO2 were reduced in the yid1 and med25 mutants under iron-deficient conditions. The finding that YID1/MED16 and MED25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.

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A novel iron-regulated metal transporter from plants identified by functional expression in yeast.

Cited by 1,247 publications

References 34 publications

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Genetic engineering in the improvement of plants for phytoremediation of metal polluted soils

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

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