A novel ion pairing LC/MS metabolomics protocol for study of a variety of biologically relevant polar metabolites

Capillary ion chromatography–mass spectrometry for simultaneous determination of glucosylglycerol and sucrose in intracellular extracts of cyanobacteria

“…Significantly Table 2). This result indicated that the sensitivity significantly increased with capillary IC-MS compared with HPLC-MS methods based on ion-pair chromatography [23] or hydrophilic interaction and [24] anion-exchange IC-MS method using 4 or 2 mm i.d. columns [25][26][27].…”

Quantifying intracellular metabolites in yeast using a matrix with minimal interference from naturally occurring analytes

“…Six matrix blanks were analyzed during the quantification. The average peak area of each metabolite, detected in the matrix blanks, was subtracted from the peak area of that metabolite identified in the spiked samples, to correct for the naturally occurring amount of the particular metabolite [12]. Weighted linear regression (weighted to 1/x) was used to establish the linearity for each of the compounds.…”

“…Furthermore, most of the intracellular metabolites are ionic and highly polar, providing a formidable analytical challenge from a separation perspective. As a consequence, sensitive and very selective analytical methods are required, such as liquid chromatography tandem mass spectrometry (LC-MS/MS), gas chromatography MS/MS (GC-MS/MS), and capillary electrophoresis MS/MS (CE-MS/MS) [12][13][14]. However, GC-MS is generally not a suitable analytical technique for measurement of large polar molecules such as di-and triphosphorylated nucleotides, NAD + , and NADH [15] because they decompose during injection even when derivatized.…”

Metabolomic Profiling of Anionic Metabolites in Head and Neck Cancer Cells by Capillary Ion Chromatography with Orbitrap Mass Spectrometry

“…14,15 However, ionic and very polar compounds are unable to be retained on a conventional reversed-phase (RP) column. Recently, an ion-pairing LC (IPLC) method has been demonstrated by introducing certain ion-pairing agents e.g., diamyl ammonium 16 or tributylamine 17 to improve chromatographic retention for polar molecules. On the other hand, the hydrophilic interaction chromatography (HILIC) method has emerged as an alternative to RPLC and shows promise in separating polar compounds.…”