A novel intracellular pH-responsive formulation for FTY720 based on PEGylated graphene oxide nano-sheets

Masoudipour, Elham; Kashanian, Soheila; Maleki, Nasim; Karamyan, Ali; Omidfar, Kobra

doi:10.1080/03639045.2017.1386194

Cited by 15 publications

(14 citation statements)

References 45 publications

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“…Cytotoxicity investigations were also performed for different types of FTY720 NPs, including graphene oxide, liposomal, and calcium phosphate-based NPs. 21 , 22 , 50 As observed here, these authors also reported that cell viability decreased in a concentration-dependent manner upon incubation with FTY720 NPs.…”

Section: Resultssupporting

confidence: 85%

Modulating Fingolimod (FTY720) Anti-SARS-CoV-2 Activity Using a PLGA-Based Drug Delivery System

Miranda

Ferreira

Souza

et al. 2022

ACS Appl. Bio Mater.

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COVID-19 has resulted in more than 490 million people being infected worldwide, with over 6 million deaths by April 05th, 2022. Even though the development of safe vaccine options is an important step to reduce viral transmission and disease progression, COVID-19 cases will continue to occur, and for those cases, efficient treatment remains to be developed. Here, a drug repurposing strategy using nanotechnology is explored to develop a therapy for COVID-19 treatment. Nanoparticles (NPs) based on PLGA for fingolimod (FTY720) encapsulation show a size of ∼150 nm and high drug entrapment (∼90%). The NP (NP@FTY720) can control FTY720 release in a pH-dependent manner. Cytotoxicity assays using different cell lines show that NP@FTY720 displays less toxicity than the free drug. Flow cytometry and confocal microscopy reveal that NPs are actively internalized mostly through caveolin-mediated endocytosis and macropinocytosis pathways and co-localized with lysosomes. Finally, NP@FTY720 not only exhibits anti-SARS-CoV-2 activity at non-cytotoxic concentrations, but its biological potential for viral infection inhibition is nearly 70 times higher than that of free drug treatment. Based on these findings, the combination of drug repurposing and nanotechnology as NP@FTY720 is presented for the first time and represents a promising frontline in the fight against COVID-19.

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Section: Resultssupporting

confidence: 85%

Modulating Fingolimod (FTY720) Anti-SARS-CoV-2 Activity Using a PLGA-Based Drug Delivery System

Miranda

Ferreira

Souza

et al. 2022

ACS Appl. Bio Mater.

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“…The conservation of the carbon‐oxygen double bonds in mrGO, shown through the XPS measurements, could be a valuable source for chemical modifications, which are necessary for most biosensors . At the same time, the mrGO is up to 2.7 magnitudes more conductive than graphene oxide, which would enable applications requiring both chemical modification and electrical measurements (e. g. for field‐effect transistor biosensors) . The lower proportion of carbon‐oxygen single bonds in mrGO compared to GO could decrease the amount of accidentally‐bound biomolecules during biosensing in comparison to GO and, therefore, increase the selectivity of biosensors made from mrGO in comparison to GO .…”

Section: Discussionmentioning

confidence: 99%

“…[42] At the same time, the mrGO is up to 2.7 magnitudes more conductive than graphene oxide, which would enable applications requiring both chemical modification and electrical measurements (e. g. for field-effect transistor biosensors). [42,43] The lower proportion of carbon-oxygen single bonds in mrGO compared to GO could decrease the amount of accidentallybound biomolecules during biosensing in comparison to GO and, therefore, increase the selectivity of biosensors made from mrGO in comparison to GO. [44] The improved storability of mrGO in water coupled with the 2.7 orders-of-magnitude increase in conductance may be beneficial for conductive inks as well.…”

Section: Discussionmentioning

confidence: 99%

Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis

et al. 2019

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Graphene's maximized surface‐to‐volume ratio, high conductance, mechanical strength, and flexibility make it a promising nanomaterial. However, large‐scale graphene production is typically cost‐intensive. This manuscript describes a microbial reduction approach for producing graphene that utilizes the bacterium Shewanella oneidensis in combination with modern nanotechnology to enable a low‐cost, large‐scale production method. The bacterial reduction approach presented in this paper increases the conductance of single graphene oxide flakes as well as bulk graphene oxide sheets by 2.1 to 2.7 orders of magnitude respectively while simultaneously retaining a high surface‐area‐to‐thickness ratio. Shewanella ‐mediated reduction was employed in conjunction with electron‐beam lithography to reduce one surface of individual graphene oxide flakes. This methodology yielded conducting flakes with differing functionalization on the top and bottom faces. Therefore, microbial reduction of graphene oxide enables the development and up‐scaling of new types of graphene‐based materials and devices with a variety of applications including nano‐composites, conductive inks, and biosensors, while avoiding usage of hazardous, environmentally‐unfriendly chemicals.

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“…Introducing tunable material properties of degradability and stiffness could directly influence neural progenitor cell behaviour [ 106 ] and influence adipose derived stem cell chondrogenic differentiation [ 107 ]. A pH-responsive nanocarrier based on modified graphene oxide to promote acid-triggered intracellular release of a soluble drug illustrates the sophistication that can be envisaged, allowing triggered cell-response potency assays [ 108 ]. It is likely that more elaborate tissue-engineered biosensors may become useful in biomarker discovery as functional aspects of stem cells may be dependent on 3D derived signals that are not obtained in monolayer cultures.…”

Section: Discussionmentioning

confidence: 99%

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

Amărandi

Becheru

Vlăsceanu

et al. 2018

BioMed Research International

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Regenerative medicine is challenged by the need to conform to rigorous guidelines for establishing safe and effective development and translation of stem cell-based therapies. Counteracting widespread concerns regarding unproven cell therapies, stringent cell-based assays seek not only to avoid harm but also to enhance quality and efficacy. Potency indicates that the cells are functionally fit for purpose before they are administered to the patient. It is a paramount quantitative critical quality attribute serving as a decisive release criterion. Given a broad range of stem cell types and therapeutic contexts the potency assay often comprises one of the most demanding hurdles for release of a cell therapy medicinal product. With need for improved biomarker assessment and expedited measurement, recent advances in graphene-based biosensors suggest that they are poised to be valuable platforms for accelerating potency assay development. Among several potential advantages, they offer versatility for sensitive measurement of a broad range of potential biomarker types, cell biocompatibility for direct measurement, and small sample sufficiency, plus ease of use and point-of-care applicability.

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A novel intracellular pH-responsive formulation for FTY720 based on PEGylated graphene oxide nano-sheets

Abstract: PEGylated GO can act as a pH-responsive drug carrier to improve the efficacy of anticancer drug delivery.

Cited by 15 publications

References 45 publications

Modulating Fingolimod (FTY720) Anti-SARS-CoV-2 Activity Using a PLGA-Based Drug Delivery System

Modulating Fingolimod (FTY720) Anti-SARS-CoV-2 Activity Using a PLGA-Based Drug Delivery System

Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

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