A novel intervention on the inhibiting effects of Catunaregam spinosa induced free radical formation and DNA damage in Aedes aegypti (Diptera: Culicidae): a verdict for new perspectives on microorganism targeted vector control approach

Anoopkumar, A. N.; Rebello, Sharrel; Sudhikumar, Ambalaparambil Vasu; Puthur, Sreedev; Aneesh, Embalil Mathachan

doi:10.1007/s42690-020-00157-0

Cited by 8 publications

(5 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The insecticidal properties of the bioactive compound "squalene" were justified by. 7 In agreement with the previous findings, as discussed in the earlier sections, it was reported that the presence of the abovementioned phytochemical compounds is probably responsible for the larvicidal potential of Stachytarpheta jamaicensis since most of all the compounds have exhibited a prominent range of toxicity in previous findings. The use of environmentfriendly approaches and toxic studies to achieve benefits for humans in all aspects has significantly increased in recent years.…”

Section: Discussionsupporting

confidence: 91%

“…One of the major facts is that the ROS allied metabolism intensely influences the fecundity, immune response, and mosquitoes' vector competence. As reported in a previous study by, 7 it was noted that the augmented levels of free radicals might interrupt various biological activities, including the interruption of redox signalling pathways. Such circumstances will also cause damages to cell organelles, proteins, and nucleic acids as described by.…”

Section: Discussionmentioning

confidence: 63%

“…Based on the previous literature and research gap, it was clearly evident that the prominent use of compounds that were isolated from traditionally used medicinal plants 3,4,6 as bioherbicides have been recognised as the backbone of novel vector control strategies and are becoming an effective alternative to synthetic insecticides. 2,3,7 There exist a large number of research articles regarding the effectiveness of plant-based insecticides. However, only a few research articles, including our previous studies 2 have focussed on the probable mode of phytochemical exposure to mosquito vectors with special reference to the Reactive Oxygen Species Generation (ROS).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Rajan

2021

JCD

View full text Add to dashboard Cite

Introduction: Plant-based knowledge has been used for generations for personal protection from various mosquito species. The notion of applying such traditional perspectives in vector control research has received extensive attention in recent years. Unlike other common patterns, the present investigation has tried to explore the augmented production of reactive oxygen species (ROS) in response to Stachytarpheta jamaicensis exposure with special inference on larvicidal potential, mode of action of phytochemical compounds, and oxidative stress. Methods: The larvicidal potential was determined as per the WHO protocol. Ultraviolet-visible spectroscopy was used to determine the excessive production of ROS. GC-MS was employed to characterise the phytochemical constituents. The statistical analysis was done by using SPSS version 24.0.0. Result: The acetone extract has been found to exhibit a maximum range of toxicity in terms of larvicidal potential and reactive oxygen species formation. Among the 40 phytochemical elements characterised, Cyclopropane, 1,1,2,2-Tetramethyl; Phenyl-Acetonitrile; Pyranone; Tetradecene; Neophytadiene; Mome Inositol; Monocrotaline; and Squalene may be responsible for the augmented production of ROS in the Culex quinquefasciatus. Conclusion: The phytochemical elements in Stachytarpheta jamaicensis displayed extensive toxicity and inhibited the normal development of Culex quinquefasciatus mosquitoes by augmented production of reactive oxygen species, indicating its prominent role in oxidative stress.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Rajan

2021

JCD

View full text Add to dashboard Cite

show abstract

“…The water extract of the seed of C. spinosa was tested against human pathogenic bacteria such as Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Klebsiella pneumonia, and Proteus mirabilis ; and the minimum inhibitory concentration (MIC) was found to be 0.07, 0.08, 0.88, 3, and 0.73 mg/mL, respectively, and the zone of inhibition ranged from 15 to 20 mm [ 36 ].…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Catunaregam spinosa (Thunb.) Tirveng: A Review of Traditional Uses, Phytochemistry, Pharmacological Activities, and Toxicological Aspects

Timalsina

Devkota

Bhusal

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Catunaregam spinosa (Thunb.) Tirveng. (Syn. Randia dumetorum (Retz.) Lam.), belonging to the Rubiaceae family, is distributed in south Asian countries. It is used as a traditional medicine to treat gastrointestinal and hepatic problems and as an anti-inflammatory and antimicrobial agent. The main aim of this review is to collect and analyze the available scientific information on traditional uses, phytochemistry, and pharmacological activities of C. spinosa. The scientific information related to C. spinosa was collected from various resources and databases such as SciFinder, Scopus, PubMed, and other databases. C. spinosa was found to be an important crude drug of the traditional medicinal systems such as Ayurveda. It was found to be used by the people of India as an alternative medicine, while the fruit of this plant was found to be used in dietary regimens as well. Active phytochemicals such as catunarosides, randianin, and several other saponins and triterpenoids possess various pharmacological activities such as anti-inflammatory, hepatoprotective, antibacterial, and immunomodulatory activities. Many studies have been performed to isolate the active compounds; however, there is a need for more activity-guided isolation studies. Various in vitro studies showed promising results but there are not many studies related to mechanism of actions using animal models. Hence, future studies on C. spinosa should focus on correlating the traditional uses with active phytoconstituents and modern pharmacological activities.

show abstract

“…Emesis activity is mostly reputed pharmacological activity of C. spinosa where its extreme bitterness induces the vomiting sensation [18]. Anoopkumar, [19] studied insecticidal activity of seeds of C. spinosa on 4 th instar larvae of Aedes agypti and found LC50 as 210.212 mg L -1 [19].…”

Section: Introductionmentioning

confidence: 99%

In vitro Callus Induction of Catunaregam spinosa Using Leaves as Explant

Lawrence

Senarath²,

Munasinghe³

2022

JPRI

View full text Add to dashboard Cite

Aims: C. spinosa (Family Rubiaceae) is a valuable medicinal since years ago. A protocol was developed including best sterilization and best medium for callus induction of C. spinosa using leaf discs as explants. Methodology: Sterilization protocol optimized using different concentrations of Carbendazim® (0.2, 0.3%) and Clorox (10, 15%) exposing to different time intervals (10, 15 min). Percentage survival and contaminations were calculated. Best medium was optimized using different concentrations of 6-Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) (1.0-6.0 mg L-1). Growth regulators free Murashige and Skoog (MS) medium was used as control. Completely randomized design was followed with ten replicates in each concentration. Days taken to initiate calli, morphological characteristics and mean dry weights of calli were evaluated after 3 months of incubation. Results: Leaf discs sterilization with 0.3% Carbendazim for 10 min, 10 % Clorox for 10 min and 70% ethanol for 30 sec followed by two washings in sterile distilled water was found to be best sterilization protocol. It recorded lowest percentage contamination (13.34%) and highest percentage survival (86.66%) after 8 weeks. No observable changes were found in calli grown in growth regulators free MS medium. Calli growth and morphologies were significantly affected by the type of growth regulator and their concentrations in MS medium. Color of the calli varied from white opaque to yellow brown to green and the texture from foamy, loose, and friable to compact. Best medium with 1.0 mg L-1 BAP and 3.0 mg L-1 NAA produced green friable calli with 0.0969±0.01 g mean dry weight after 3 months. Some traits were found to be depended on synergetic effect of growth regulators and genotypic characteristics of explant. Conclusion: The study provides a better sterilization protocol and medium for in vitro calli induction and growth of C. spinosa.

show abstract

A novel intervention on the inhibiting effects of Catunaregam spinosa induced free radical formation and DNA damage in Aedes aegypti (Diptera: Culicidae): a verdict for new perspectives on microorganism targeted vector control approach

Cited by 8 publications

References 58 publications

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Catunaregam spinosa (Thunb.) Tirveng: A Review of Traditional Uses, Phytochemistry, Pharmacological Activities, and Toxicological Aspects

In vitro Callus Induction of Catunaregam spinosa Using Leaves as Explant

Contact Info

Product

Resources

About