A novel insight into the mode of action of glufosinate: how reactive oxygen species are formed

Takano, Hudson Kagueyama; Beffa, Roland; Preston, Christopher; Westra, Philip; Dayan, Franck E.

doi:10.1007/s11120-020-00749-4

Cited by 37 publications

(49 citation statements)

References 51 publications

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“…Ammonia accumulated to high levels in glufosinatetreated plants exogenously supplied with glutamine and in glufosinate-treated plants under low light, but no apparent rapid phytotoxicity was observed in either treatment. 15,58,59 A physiological comparison between mature and young leaves demonstrated that ammonia accumulated in both leaf types, but rapid phytotoxicity was observed only in older leaves. 58 Likewise, the addition of low doses of atrazine to glufosinate prevented the rapid phytotoxicity, but ammonia still accumulated to high levels in these plants.…”

Section: Relationship Between Gs Inhibition Ammonia Accumulation Andmentioning

confidence: 99%

“…58 Likewise, the addition of low doses of atrazine to glufosinate prevented the rapid phytotoxicity, but ammonia still accumulated to high levels in these plants. 59 Ammonia and other amines have been proposed as uncouplers of photophosphorylation in the light reactions of photosynthesis, ultimately decreasing carbon assimilation rates. 60 Photosynthetic uncouplers disrupt the coupling between the electron transport and phosphorylation reactions and thus inhibit ATP synthesis without affecting the respiratory chain and ATP synthase.…”

Section: Relationship Between Gs Inhibition Ammonia Accumulation Andmentioning

confidence: 99%

“…A recent breakthrough research challenged the long-accepted paradigm regarding the mode of action of glufosinate. 58,59 There is now strong evidence that reactive oxygen species (ROS) are the main driver for the rapid phytotoxicity observed in glufosinatetreated plants. The massive light-dependent production of ROS causes the catastrophic lipid peroxidation of the cell membranes, and consequently, rapid cell death.…”

Section: The Role Of Reactive Oxygen Speciesmentioning

confidence: 99%

“…Consequently, glyoxylate is not converted into glycine by the glutamate-dependent aminotransferase, which leads to glycolate and glyoxylate accumulation. 55,59 This leads to a feedback inhibition of the photorespiration pathway and subsequent inactivation of the Calvin cycle. 62 If both photorespiration and the Calvin cycle are disrupted, the equilibrium between energy generation and consumption is broken, leading to a massive oxidative stress in the chloroplasts.…”

Section: The Role Of Reactive Oxygen Speciesmentioning

confidence: 99%

“…64 In fact, plants accumulated higher levels of ROS when sprayed with glufosinate plus a low dose of dinoseb (a known uncoupler of photosynthesis) compared to glufosinate only. 59 However, the uncoupling activity of glufosinate and its physiological consequences remains to be proven.…”

Section: The Role Of Reactive Oxygen Speciesmentioning

confidence: 99%

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Glufosinate‐ammonium: a review of the current state of knowledge

Takano

Dayan

2020

Pest Management Science

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148

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Glufosinate is a key herbicide to manage glyphosate-resistant weeds mainly because it is a broad-spectrum herbicide, and transgenic glufosinate-resistant crops are available. Although glufosinate use has increased exponentially over the past decade, the treated area with this herbicide is far less than that with glyphosate. This is because glufosinate often provides inconsistent performance in the field, which is attributed to several factors including environmental conditions, application technology, and weed species. Glufosinate is also highly hydrophilic and does not translocate well in plants, generally providing poor control of grasses and perennial species. In the soil, glufosinate is rapidly degraded by microorganisms, leaving no residual activity. While there have been concerns regarding glufosinate toxicology, its proper use can be considered safe. Glufosinate is a fast-acting herbicide that was first discovered as a natural product, and is the only herbicide presently targeting glutamine synthetase. The mode of action of glufosinate has been controversial, and the causes for the rapid phytotoxicity have often been attributed to ammonia accumulation. Recent studies indicate that the contact activity of glufosinate results from the accumulation of reactive oxygen species and subsequent lipid peroxidation. Glufosinate disrupts both photorespiration and the light reactions of photosynthesis, leading to photoreduction of molecular oxygen, which generates reactive oxygen species. The new understanding of the mode of action provided new ideas to improve the herbicidal activity of glufosinate. Finally, a very few weed species have evolved glufosinate resistance in the field, and the resistance mechanisms are generally not well understood requiring further investigation.

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Section: Relationship Between Gs Inhibition Ammonia Accumulation Andmentioning

confidence: 99%

Section: Relationship Between Gs Inhibition Ammonia Accumulation Andmentioning

confidence: 99%

Section: The Role Of Reactive Oxygen Speciesmentioning

confidence: 99%

Section: The Role Of Reactive Oxygen Speciesmentioning

confidence: 99%

Section: The Role Of Reactive Oxygen Speciesmentioning

confidence: 99%

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Glufosinate‐ammonium: a review of the current state of knowledge

Takano

Dayan

2020

Pest Management Science

Self Cite

148

158

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Biological effects on Palmer amaranth surviving glufosinate

Jones

León

Everman

2022

Agrosystems Geosci & Env

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Palmer amaranth (Amaranthus palmeri S. Watson) is a difficult weed to manage due to competitive growth, fecundity, and evolved herbicide resistance. Limited information exist on the fecundity of vegetative stage Palmer amaranth surviving glufosinate applied at different timings. In addition, research has not investigated the germination or glufosinate susceptibility of the offspring from these surviving plants. Field experiments were conducted across three locations in 2019 to determine (a) the fecundity of Palmer amaranth plants surviving glufosinate applied at different vegetative growth stages, and (b) if the offspring from the surviving plants exhibited differential germination and susceptibility to glufosinate. Palmer amaranth was treated at three different vegetative sizes (5 cm, 7-10 cm, > 10 cm) and orthogonal combinations of these application timings. The application timings corresponded to early-, mid-and late-postemergence applications. Palmer amaranth plants surviving the mid-, late-, and the mid postemergence followed by late postemergence glufosinate application were fecund. Palmer amaranth plants surviving the mid postemergence followed by late postemergence application produced less seed than the plants surviving the mid postemergence and late postemergence application. Palmer amaranth was controlled with other glufosinate applications resulting in no seed production. Germination was affected across location and glufosinate treatments, but no clear trend/pattern was observed. The offspring from Palmer amaranth plants surviving glufosinate applications were controlled by all glufosinate rates tested. These experiments provide evidence that Palmer amaranth surviving glufosinate in the vegetative stages may remain fecund, but fecundity can vary with application timing. No measurable effect on the offspring germination or susceptibility to glufosinate was apparent.

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PROTAC for agriculture: learning from human medicine to generate new biotechnological weed control solutions

Leon,

Bassham

2023

Pest Management Science

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Weed control has relied on the use of organic and inorganic molecules that interfere with druggable targets, especially enzymes, for almost a century. This approach, although effective, has resulted in multiple cases of herbicide resistance. Furthermore, the rate of discovery of new druggable targets that are selective and with favorable environmental profiles has slowed down, highlighting the need for innovative control tools. The arrival of the biotechnology and genomics era gave hope to many that all sorts of new control tools would be developed. However, the reality is that most efforts have been limited to the development of transgenic crops with resistance to a few existing herbicides, which in fact is just another form of selectivity. Proteolysis‐targeting chimera (PROTAC) is a new technology developed to treat human diseases but that has potential for multiple applications in agriculture. This technology uses a small bait molecule linked to an E3 ligand. The 3‐dimensional structure of the bait favors physical interaction with a binding site in the target protein in a manner that allows E3 recruitment, ubiquitination and then proteasome‐mediated degradation. This system makes it possible to circumvent the need to find druggable targets because it can degrade structural proteins, transporters, transcription factors, and enzymes without the need to interact with the active site. PROTAC can help control herbicide‐resistant weeds as well as expand the number of biochemical targets that can be used for weed control. In the present article, we provide an overview of how PROTAC works and describe the possible applications for weed control as well as the challenges that this technology might face during development and implementation for field uses.This article is protected by copyright. All rights reserved.

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A novel insight into the mode of action of glufosinate: how reactive oxygen species are formed

Cited by 37 publications

References 51 publications

Glufosinate‐ammonium: a review of the current state of knowledge

Glufosinate‐ammonium: a review of the current state of knowledge

Biological effects on Palmer amaranth surviving glufosinate

PROTAC for agriculture: learning from human medicine to generate new biotechnological weed control solutions

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