A novel insight into the key gene signature associated with the immune landscape in the progression of sarcopenia

Shen, Zi-Le; Chen, Wen-Hao; Liu, Zhang; Yu, Ding-Ye; Chen, Wei-Zhe; Zang, Wang-Fu; Zhang, Peng; Yan, Xia-Lin; Yu, Zhen

doi:10.1016/j.exger.2023.112244

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…In patients with sarcopenia, T helper 17 (Th17) cells are down-regulated, while NK cells are up-regulated. Furthermore, certain key genes regulate the progression of sarcopenia by influencing the immune microenvironment (Shen et al, 2023). When muscle is damaged, resident neutrophils and macrophages become activated, and the cytokines and growth factors released by activated T cells play a pivotal role in the proliferation and migration of muscle is the author/funder, who has granted medRxiv a license to display the preprint in (which was not certified by peer review) satellite cells (Saini, McPhee, Al-Dabbagh, Stewart, & Al-Shanti, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterizing the skeletal muscle immune microenvironment for sarcopenia using transcriptome analysis and histological validation

Shen,

Zong,

Zhao

et al. 2024

Preprint

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Sarcopenia is a condition characterized by the age-related loss of skeletal muscle mass and function. The pathogenesis of the disease is influenced by chronic low-grade inflammation. However, the specific changes in the immune landscape changes of sarcopenic muscle are not yet fully understood. To gain insights into the immune cell composition and interactions, we combined single-nuclei RNA sequencing data, bulk RNA sequencing datasets, and comprehensive bioinformatic analyses on skeletal muscle samples from young, aged, and sarcopenic individuals. Histological staining was then performed on skeletal muscles to validate the distribution of immune cells in clinical samples. Overall, we analyzed the transcriptomes of 101,862 single nuclei, revealing a total of 10 major cell types and 6 subclusters of immune cell types within the human skeletal muscle tissues. Among the immune cells, macrophages constituted the largest immune fraction. A specific marker gene LYVE1 for skeletal muscle macrophages was further identified. Cellular subclasses included four distinct groups of resident macrophages, which play a different role in physiological or non-physiological conditions. Using bulk RNA sequencing data, we identified strong enrichment for a macrophage-rich inflammation in sarcopenia. Our findings demonstrate age-related changes in the composition and cross-talk of immune cells, which contribute to chronic inflammation. Furthermore, macrophages emerge as a potential therapeutic target, thus advancing our understanding of the pathogenesis of sarcopenia.

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Section: Introductionmentioning

confidence: 99%

Characterizing the skeletal muscle immune microenvironment for sarcopenia using transcriptome analysis and histological validation

Shen,

Zong,

Zhao

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In patients with sarcopenia, T helper 17 (Th17) cells are down-regulated, while NK cells are up-regulated. Furthermore, certain key genes regulate the progression of sarcopenia by influencing the immune microenvironment ( 16 ). When muscle is damaged, resident neutrophils and macrophages become activated, and the cytokines and growth factors released by activated T cells play a vital role in the proliferation and migration of muscle satellite cells ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

Characterizing the skeletal muscle immune microenvironment for sarcopenia: insights from transcriptome analysis and histological validation

Shen,

Zong,

Zhao

et al. 2024

Front. Immunol.

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BackgroundSarcopenia is a condition characterized by the age-related loss of skeletal muscle mass and function. The pathogenesis of the disease is influenced by chronic low-grade inflammation. However, the specific changes in the immune landscape changes of sarcopenic muscle are not yet fully understood.MethodsTo gain insights into the immune cell composition and interactions, we combined single-nucleus RNA sequencing data, bulk RNA sequencing dataset, and comprehensive bioinformatic analyses on the skeletal muscle samples from young, aged, and sarcopenic individuals. Histological staining was then performed on skeletal muscles to validate the distribution of immune cells in clinical samples.ResultsWe analyzed the transcriptomes of 101,862 single nuclei, revealing a total of 10 major cell types and 6 subclusters of immune cell types within the human skeletal muscle tissues. Notable variations were identified in the immune microenvironment between young and aged skeletal muscle. Among the immune cells from skeletal muscle microenvironment, macrophages constituted the largest fraction. A specific marker gene LYVE1 for skeletal muscle resident macrophages was further identified. Cellular subclasses included four distinct groups of resident macrophages, which play different roles in physiological or non-physiological conditions. Utilizing bulk RNA sequencing data, we observed a significant enrichment of macrophage-rich inflammation in sarcopenia.ConclusionsOur findings demonstrate age-related changes in the composition and cross-talk of immune cells in human skeletal muscle microenvironment, which contribute to chronic inflammation in aged or sarcopenia muscle. Furthermore, macrophages emerge as a potential therapeutic target, thus advancing our understanding of the pathogenesis of sarcopenia.

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Polygonatum sibiricum polysaccharide ameliorates skeletal muscle aging via mitochondria-associated membrane-mediated calcium homeostasis regulation

Chen,

Shen,

Dong

et al. 2024

Phytomedicine

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A novel insight into the key gene signature associated with the immune landscape in the progression of sarcopenia

Cited by 3 publications

References 51 publications

Characterizing the skeletal muscle immune microenvironment for sarcopenia using transcriptome analysis and histological validation

Characterizing the skeletal muscle immune microenvironment for sarcopenia using transcriptome analysis and histological validation

Characterizing the skeletal muscle immune microenvironment for sarcopenia: insights from transcriptome analysis and histological validation

Polygonatum sibiricum polysaccharide ameliorates skeletal muscle aging via mitochondria-associated membrane-mediated calcium homeostasis regulation

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