A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze]

Abstract

Background Tea plant (Camellia sinensis) is one of the most popular non-alcoholic beverage worldwide. Lateral roots (LRs) of tea plant are the main organ used for tea plant to absorb soil moisture and nutrients. Lateral roots formation and development are tightly regulated by the nitrogen and auxin signaling pathway. In order to understand the function of auxin and nitrogen signaling in LRs formation and development, transcriptome analysis was applied to investigate the differentially expressed genes involved in lateral roots of tea plants treated with indole-3-butyric acid (IBA), N-1-naphthylphthalamic acid (NPA), low and high nitrogen concentration. Results A total of 296 common differentially expressed genes were mainly identified and annotated to four signaling pathways, such as nitrogen metabolism, plant hormone signal transduction, Glutathione metabolism and transcription factors. RNA-sequencing results revealed that majority of differentially expressed genes play important roles in nitrogen metabolism and hormonal signal transduction. Low nitrogen condition induced the biosynthesis of auxin and accumulation of transcripts, thereby regulating lateral roots formation. Furthermore, metabolism of cytokinin and ethylene biosynthesis were also involved in lateral roots development. Transcription factors like MYB genes also contributed to the lateral roots formation of tea plants through secondary cell wall biosynthesis. Reversed phase ultra performance liquid chromatography (RP-UPLC) results showed that the auxin concentration in lateral roots was increased, while the nitrogen level decreased. Thus, tea plant lateral roots formation could be induced by low nitrogen concentration via auxin biosynthesis and accumulation. Conclusion This study provides new insights into the mechanisms associated with nitrogen and auxin signaling pathways to regulate LRs formation and arises new clues for the efficient utilization of nitrogen in tea plant at the genetic level.

Transcriptome analysis reveals multiple effects of nitrogen accumulation and metabolism in the roots, shoots, and leaves of potato (Solanum tuberosum L.)

“…It plays its regulatory role primarily by inducing the expression of early auxin response genes. The low nitrogen condition induced the biosynthesis of auxin and accumulation of transcripts, and the source of auxin or auxin transport revealed a role for auxin in regulating N remobilization [ 58 , 59 ]. Using WGCNA and co-expression methodology, we identified many DEGs involved in auxin -induced and auxin-regulated (Figs.…”

Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency

“…Excessive exogenous IAA treatment can down-regulate TAR2 to balance the auxin concentration in lateral roots. Auxin concentration increased with the decrease of nitrogen level, and the formation of lateral roots could be induced by the auxin biosynthesis and accumulation under LN stress [53,54]. In this study, the expression of TaSAUR66-5B was rapidly up-regulated by IAA treatment (Figure 4A), while overexpression of TaSAUR66-5B also promoted the biosynthesis of auxin (Figure 5E-H).…”

Transcriptome Analysis Reveals Multiple Effects of Nitrogen Accumulation and Metabolism in the Roots, Shoots, and Leaves of Potato (Solanum Tuberosum L.)

“…It plays its regulatory role primarily by inducing the expression of early auxin response genes. The source of auxin or auxin transport discovered a task for growth regulator in regulation N remobilization (Hu et al 2020;Li et al 2020). Using WGCNA and co-expression methodology, we tend to known many DEGs involved in Auxin-induced and Auxin-regulated (Figs.…”