A novel inhibitor of NADH dehydrogenase in <i>Paracoccus denitrificans</i>

Phillips, Mary K.; Kell, Douglas B.

doi:10.1016/0014-5793(82)80905-8

Cited by 13 publications

(5 citation statements)

References 6 publications

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“…denitrijicans resulted in an immediate cessation of growth, followed after about 1 h by a recovery of growth (Meijer et al, 1978). We have confirmed these findings (Phillips & Kell, 1981, 1982 both for rotenone, and also for antimycin A (Phillips, 1983), a segment I1 inhibitor. An immediate cessation of growth occurred when rotenone was added to give concentrations of 1 0 0~~ (300 nmol mg dry wt-l) or 200 p~ (600 nmol mg dry wt-l); however, in both cases, growth resumed after about 1 h at rates similar to those attained in the absence of rotenone (Meijer et al, 1978 ;Phillips & Kell, 1982).…”

Section: Effect Of Tinopal An On the Growth Kinetics Of P Denitrijisupporting

confidence: 79%

“…We have confirmed these findings (Phillips & Kell, 1981, 1982 both for rotenone, and also for antimycin A (Phillips, 1983), a segment I1 inhibitor. An immediate cessation of growth occurred when rotenone was added to give concentrations of 1 0 0~~ (300 nmol mg dry wt-l) or 200 p~ (600 nmol mg dry wt-l); however, in both cases, growth resumed after about 1 h at rates similar to those attained in the absence of rotenone (Meijer et al, 1978 ;Phillips & Kell, 1982). Similar effects were recently reported using the electron transport inhibitor myxothiazol (Thierbach & Reichenbach, 1983).…”

Section: Effect Of Tinopal An On the Growth Kinetics Of P Denitrijisupporting

confidence: 74%

“…Further experiments (Phillips & Kell, 1982) were carried out to determine whether Tinopal AN acted at the same site as other segment I inhibitors such as rotenone, amytal and piericidin A, which were all shown to bind to the same polypeptide (Horgan et al, 1968), namely the iron-sulphur centre N-2 (Meijer et al, 1978). The results showed that Tinopal AN did not share the same specific binding site as rotenone (and also presumably amytal and piericidin A), and hence provided the first example of an inhibitor of the NADH dehydrogenase region of the respiratory chain whose binding site was not associated with iron-sulphur centre N-2 (Phillips & Kell, 1982). Maguire et al (1983) subsequently reported that the respiratory block by Tinopal AN occurred on the oxidizing side of cluster N-2 in submitochondrial particles.…”

Section: Introductionmentioning

confidence: 98%

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The Antibacterial Action of Tinopal AN

1984

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The bactericidal activity of Tinopal AN [1,1-bis(3,N-5-dimethyl-benzoxazol-2-yl)-methine p-toluene sulphonate] was shown to be due to a mechanism entirely independent of its inhibitory effects upon NADH dehydrogenase which were reported previously. Whereas the compound had no significant effect upon DNA synthesis in Escherichia coli D22, RNA and protein synthesis were immediately and markedly inhibited. In confirmation, Tinopal AN caused an immediate cessation in inducible beta-galactosidase synthesis in the same organism. An in vitro assay of the transcription of calf-thymus DNA by purified E. coli RNA polymerase showed that this process was inhibited by Tinopal AN.

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Section: Effect Of Tinopal An On the Growth Kinetics Of P Denitrijisupporting

confidence: 79%

Section: Effect Of Tinopal An On the Growth Kinetics Of P Denitrijisupporting

confidence: 74%

Section: Introductionmentioning

confidence: 98%

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The Antibacterial Action of Tinopal AN

1984

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“…Tinopal AN was previously shown to be a potent inhibitor of certain bacterial NADH dehydrogenases (Phillips & Kell 1981, 1982, and of RNA and thus protein synthesis (Phillips et a!. 1984).…”

Section: Discussionmentioning

confidence: 99%

Tinopal AN as a selective agent for the differentiation of phytopathogenic and saprophytic Pseudomonas species

Phillips

Roberts

Kell

1985

Journal of Applied Bacteriology

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The selective toxicity of the respiratory inhibitor Tinopal AN (1,1‐bis (3,N‐5–dimethylbenzoxazol‐2‐yl) methine p‐toluene sulphonate) towards phytopathogenic bacteria was investigated further and in general was confirmed using more than 160 additional strains of Pseudomonas spp. The mechanism(s) of the resistance shown by saprophytic fluorescent pseudomonads were studied to elucidate the differences between resistant saprophytic and sensitive phytopathogenic Pseudomonas species. Damage to, or partial removal of the cell wall of Tinopal AN‐resistant Pseudomonas aeruginosa, resulted in a marked Tinopal AN‐sensitivity, as judged by the ability of Tinopal AN to inhibit oxygen uptake. Furthermore, removal of part of the lipo‐polysaccharide (LPS) component of the outer membrane also resulted in sensitivity. Mutants of Ps. aeruginosa with modified outer cell walls were tested for their reactions towards Tinopal AN, and two cell wall lipopolysaccharide (LPS) mutants of Escherichia coli (env Al) and Salmonella typhimurium (rfa) were, in contrast to the wild‐type strains, found to be sensitive towards Tinopal AN. The results therefore suggest that the resistance of saprophytic pseudomonads towards Tinopal AN is due (at least in part) to the selectively permeable properties of the outer membrane of the cell wall. The usefulness of Tinopal AN for screening potentially phytopathogenic strains of Pseudomonas was confirmed.

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“…Fluorescent brighteners are stains which are widely used as additives in domestic washing powders to improve the apparent ''whiteness'' of clothes; they exert this effect by absorbing otherwise invisible UV light and giving off a blue fluorescence (11,38). They have occasionally been used in fluorescence microscopy (21) and are known to interact with a number of microbial cells (22)(23)(24)(25). Some, such as calcofluor white, are well-known stains of the fungal cell wall, and have been exploited in flow cytometric analyses (13).…”

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confidence: 99%

Fluorescent brighteners: Novel stains for the flow cytometric analysis of microorganisms

Davey¹,

Kell²

1997

Cytometry

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Flow cytometry is a rapid method for measuring the optical properties of individual cells. The technique has found great utility in the study of mammalian cells, but microbiological applications have been more limited. We here show that UV-excited fluorescent whitening agents, in particular Tinopal CBS-X, are effective stains for both vegetative microbial cells and for spores of Gram-positive bacteria. Pretreatment of samples with ethanol speeds the staining process. Under favourable conditions, Tinopal CBS-X may be used to discriminate among organisms, a fact that may be useful when screening for a target microorganism against a high biological background. Cytometry 28:311-315, 1997.

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A novel inhibitor of NADH dehydrogenase in Paracoccus denitrificans

Cited by 13 publications

References 6 publications

The Antibacterial Action of Tinopal AN

The Antibacterial Action of Tinopal AN

Tinopal AN as a selective agent for the differentiation of phytopathogenic and saprophytic Pseudomonas species

Fluorescent brighteners: Novel stains for the flow cytometric analysis of microorganisms

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