A novel inexpensive electrochemical sensor for pyrazinoic acid as a potential tool for the identification of pyrazinamide-resistant Mycobacterium tuberculosis

Azagra, Daniel; Furukawa, Roberto; Fuentes, Patricia; Comina, Germán; Castro, Nicolas Rey de; Requena, David; Gilman, Robert H.; Sheen, Patricia; Zimic, Mirko

doi:10.4103/ijmy.ijmy_63_18

Cited by 4 publications

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“…The obtained slopes from the current vs pH in the pH range of 3.0–9.0 indicates equal number of protons and electrons are participating in the reduction of ETB and PZM as described previously 32 .…”

Section: Resultsmentioning

confidence: 95%

Simultaneous detection of ethambutol and pyrazinamide with IL@CoFe2O4NPs@MWCNTs fabricated glassy carbon electrode

Chokkareddy

Kanchi

Inamuddin

2020

Sci Rep

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For the first time, we report a novel electrochemical sensor for the simultaneous detection of ethambutol (ETB) and pyrazinamide (PZM) using 1-ethyl-3-methylimidazolium tetrafluoroborate ([emim][Bf 4 ]) ionic liquid (iL) assimilated with multiwalled carbon nanotubes (MWcnts) decorated cobalt ferrite nanoparticles (cofe 2 o 4 nps) on the surface of glassy carbon electrode (Gce). the surface morphological and electrochemical properties of the iL@cofe 2 o 4 nps@MWcnts was characterized with X-ray diffraction (XRD), transmission electron microscope (TEM), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry (CV), differential pulse voltammetry (DPV) respectively. Moreover, the obtained results of CV demonstrated that the 9-folds enhancement in the electrochemical signals was achieved with iL@cofe 2 o 4 nps@MWcnts@Gce compared to that of a bare Gce. Additionally, the simultaneous electrochemical detection of etB and pZM was successfully accomplished using iL@cofe 2 o 4 NPs@MWCNTs over a wide-range of concentration with good limit of detection (3S/m) of 0.0201 and 0.010 μM respectively. The findings of this study identify iL@cofe 2 o 4 nps@MWcnts@Gce has promising abilities of simultaneous detection of etB and pZM in pharmaceutical formulations.

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Section: Resultsmentioning

confidence: 95%

Simultaneous detection of ethambutol and pyrazinamide with IL@CoFe2O4NPs@MWCNTs fabricated glassy carbon electrode

Chokkareddy

Kanchi

Inamuddin

2020

Sci Rep

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The overview and perspectives of biosensors and Mycobacterium tuberculosis: A systematic review

Rizi

Aryan

Meshkat

et al. 2020

Journal Cellular Physiology

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Tuberculosis (TB) is referred to as a "consumption" or phthisis, which has been a fatal human disease for thousands of years. Mycobacterium tuberculosis (M. tb) might have been responsible for the death of more humans than any other bacterial pathogens. Therefore, the rapid diagnosis of this bacterial infection plays a pivotal role in the timely and appropriate treatment of the patients, as well as the prevention of disease spread. More than 98% of TB cases are reported in developing countries, and due to the lack of well-equipped and specialized diagnostic laboratories, development of effective diagnostic methods based on biosensors is essential for this bacterium. In this review, original articles published in English were retrieved from multiple databases, such as PubMed, Scopus, Google Scholar, Science Direct, and Cochrane Library during January 2010-October 2019. In addition, the reference lists of the articles were also searched. Among 109 electronically searched citations, 42 articles met the inclusion criteria. The highest potential and wide usage of biosensors for the diagnosis of M. tb and its drug resistance belonged to DNA electrochemical biosensors (isoniazid and rifampin strains). Use of biosensors is expanding for the detection of resistant strains of anti-TB antibiotics with high sensitivity and accuracy, while the speed of these sensory methods is considered essential as well. Furthermore, the lowest limit of detection (0.9 fg/ml) from an electrochemical DNA biosensor was based on graphene-modified iron-oxide chitosan hybrid deposited on fluorine tin oxide for the MPT64 antigen target. According to the results, the most common methods used for M. tb detection include acid-fast staining, cultivation, and polymerase chain reaction (PCR). Although molecular techniques (e.g., PCR and real-time PCR) are rapid and sensitive, they require sophisticated laboratory and apparatuses, as well as skilled personnel and expertise in the commentary of the results. Biosensors are fast, valid, and cost-efficient diagnostic method, and the improvement of their quality is of paramount importance in resource-constrained settings.

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Electrochemical Techniques-Based approaches for Mycobacterium Tuberculosis Detection: Last Decade Review

Alfarhan

Zakaria

Yusof³

et al. 2019

IOP Conf. Ser.: Mater. Sci. Eng.

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Mycobacterium Tuberculosis (MTB) is a common airborne infectious disease that leads to millions of deaths every year worldwide. It is still one of the top ten causes of death and the victims of TB are more than HIV/AIDS in 2017. Traditional approaches for MTB detection are either take a long time, unreliable or high cost. The electrochemical techniques (ECTs) as improved and inexpensive approaches to detect the MTB. Many of ECTs were used in MTB detection such as differential pulse voltammetric (DPV), cyclic voltammetric (CV), square wave voltammetric (SWV), amperometric and impedimetric. Principle of the MTB detection using ECTs depends on DNA hybridization of the MTB on the working electrode of ECTs. The researchers developed biosensors or aptasensors and used them for ECTs analyzing to detect the MTB. They developed various biosensors from various composite and DNA probes but all the developed composite of the biosensors were used to coat the electrodes that used in ECTs. Many types of electrodes and electrolytes were used in MTB detection. The most used ECTs in MTB detection is DPV and CV while the least used is amperometric. The ECTs for MTB detection achieved high sensitivity, reliability, low detection time and very low detection limits.

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A novel inexpensive electrochemical sensor for pyrazinoic acid as a potential tool for the identification of pyrazinamide-resistant Mycobacterium tuberculosis

Abstract: Inexpensive and reusable electrochemical sensors with a portable potentiostat are a promising tool for the detection of POA, a biomarker of PZA susceptible M. Tuberculosis.

Cited by 4 publications

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Simultaneous detection of ethambutol and pyrazinamide with IL@CoFe2O4NPs@MWCNTs fabricated glassy carbon electrode

Simultaneous detection of ethambutol and pyrazinamide with IL@CoFe2O4NPs@MWCNTs fabricated glassy carbon electrode

The overview and perspectives of biosensors and Mycobacterium tuberculosis: A systematic review

Electrochemical Techniques-Based approaches for Mycobacterium Tuberculosis Detection: Last Decade Review

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