Abstract:the aim of our study was to develop a novel approach to investigating mouse detrusor smooth muscle cell (SMc) physiological activity, utilizing an acute tissue dissection technique and confocal calcium imaging. The bladder of a sacrificed adult female NMRI mouse was dissected. We used light and transmission electron microscopy to assess morphology of SMcs within the tissue. calcium imaging in individual SMcs was performed using confocal microscopy during stimulation with increasing concentrations of carbamylch… Show more
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