A novel impedimetric sensor for detecting LAMP amplicons of pathogenic DNA based on magnetic separation

Sharif, Sumaira; Wang, Yixian; Ye, Zunzhong; Wang, Zhen; Qiu, Qimin; Ying, Shengna

doi:10.1016/j.snb.2019.127051

Cited by 24 publications

(13 citation statements)

References 33 publications

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“…Polyaniline@graphene oxide nanocomposites were used to functionalize the sensing electrodes, and the microfluidic impedance sensors could detect down to 0.12 ppb. Sharif et al integrated a microfluidic system and a magnetic separation procedure to develop a novel impedance sensor for the detection of various foodborne pathogens [44]. Their results showed that the impedance sensor was effective for the detection of various foodborne pathogens, including Escherichia coli (E. coli O157:H7), Vibrio parahaemolyticus (V. parahaemolyticus), Staphylococcus aureus (S. aureus), and Listeria monocytogenes (L. monocytogenes).…”

Section: Polymer-based Impedance Biosensorsmentioning

confidence: 99%

A Review on Microfluidics-Based Impedance Biosensors

et al. 2023

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Electrical impedance biosensors are powerful and continuously being developed for various biological sensing applications. In this line, the sensitivity of impedance biosensors embedded with microfluidic technologies, such as sheath flow focusing, dielectrophoretic focusing, and interdigitated electrode arrays, can still be greatly improved. In particular, reagent consumption reduction and analysis time-shortening features can highly increase the analytical capabilities of such biosensors. Moreover, the reliability and efficiency of analyses are benefited by microfluidics-enabled automation. Through the use of mature microfluidic technology, complicated biological processes can be shrunk and integrated into a single microfluidic system (e.g., lab-on-a-chip or micro-total analysis systems). By incorporating electrical impedance biosensors, hand-held and bench-top microfluidic systems can be easily developed and operated by personnel without professional training. Furthermore, the impedance spectrum provides broad information regarding cell size, membrane capacitance, cytoplasmic conductivity, and cytoplasmic permittivity without the need for fluorescent labeling, magnetic modifications, or other cellular treatments. In this review article, a comprehensive summary of microfluidics-based impedance biosensors is presented. The structure of this article is based on the different substrate material categorizations. Moreover, the development trend of microfluidics-based impedance biosensors is discussed, along with difficulties and challenges that may be encountered in the future.

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Section: Polymer-based Impedance Biosensorsmentioning

confidence: 99%

A Review on Microfluidics-Based Impedance Biosensors

et al. 2023

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“…The magnetic-based isolation approach exploits magnetic particles to extract nucleic acids at the circumstance of external magnetic fields. The commonly utilized magnetic particles include silica-coated, amino-coated, and carboxyl-coated Fe 3 O 4 or γ-Fe 2 O 3 magnetic particles [33][34][35][36]. Compared with silicon-based, solid-phase extraction techniques, a distinct feature of the magnetic particles is ease of manipulation and control using an external magnet.…”

Section: Magnetic-based Strategiesmentioning

confidence: 99%

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Wang

Hong

et al. 2022

Military Med Res

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Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

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“…[8a,38] Among various electrochemical techniques used for the determination of microbes, impedimetric sensors have attracted wealth of attention of researchers. [39] Various impedance approaches have been applied to detect bacteria but electrochemical technique which measures electron-transfer resistance change for bacterial detection, is the most commonly used strategy. In this regards, Wan et al fabricated agglutination assay based impedimetric immunosensor for fast and label-free detection of SRB concentration.…”

Section: Impedimetric Immunosensorsmentioning

confidence: 99%

Turning the Page: Advancing Detection Platforms for Sulfate Reducing Bacteria and their Perks

Asif

Aziz

Ashraf

et al. 2021

The Chemical Record

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Sulfate reducing bacteria (SRB) are blamed as main culprits in triggering huge corrosion damages by microbiologically influenced corrosion. They obtained their energy through enzymatic conversion of sulfates to sulfides which are highly corrosive. However, conventional SRB detection methods are complex, time‐consuming and are not enough sensitive for reliable detection. The advanced biosensing technologies capable of overcoming the aforementioned drawbacks are in demand. So, nanomaterials being economical, environmental friendly and showing good electrocatalytic properties are promising candidates for electrochemical detection of SRB as compared with antibody based assays. Here, we summarize the recent advances in the detection of SRB using different techniques such as PCR, UV visible method, fluorometric method, immunosensors, electrochemical sensors and photoelectrochemical sensors. We also discuss the SRB detection based on determination of sulfide, typical metabolic product of SRB.

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A novel impedimetric sensor for detecting LAMP amplicons of pathogenic DNA based on magnetic separation

Cited by 24 publications

References 33 publications

A Review on Microfluidics-Based Impedance Biosensors

A Review on Microfluidics-Based Impedance Biosensors

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Turning the Page: Advancing Detection Platforms for Sulfate Reducing Bacteria and their Perks

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