A novel impedimetric immunosensor for the detection of chicken interferon-gamma based on a polythionine and gold nanoparticle modified glassy carbon electrode

Dai, Hua; Yang, Gongjun; Qi, Fen; Jiao, Xinan

doi:10.1039/c3ay40820e

Cited by 9 publications

(3 citation statements)

References 53 publications

(45 reference statements)

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“…The calibration curves of the fabricated Fe 3 O 4 -Au signal-amplified immunosensor for multiple detection of ChIL-4 and ChIFN-γ are shown in Figure B, C. It is obvious that the CL intensities increased linearly with the increase of both concentrations ranging from 0.0050 to 0.10 ng/mL (ChIL-4) and 0.0050 to 0.10 ng/mL (ChIFN-γ) with the linear regression equations of I CL = 5082.15 + 192936[ChIL-4] ( R 2 = 0.9962) and I CL = 5989.43 + 193277[ChIFN-γ] ( R 2 = 0.9994). The detection limits were calculated to be 0.58 pg/mL (ChIL-4) and 0.47 pg/mL (ChIFN-γ) at signal-noise ratio of 3, which are much lower than the previous literature values for detection of ChIL-4 or ChIFN-γ. − This demonstrates that a functional zonation strategy of nanozyme (Fe 3 O 4 -Au heterodimer) as signal detection probe enhanced the sensitivity of the CL imaging immunosensor.…”

Section: Resultsmentioning

confidence: 79%

Functional Zonation Strategy of Heterodimer Nanozyme for Multiple Chemiluminescence Imaging Immunoassay

Shi,

Peng,

Zhu

et al. 2023

Anal. Chem.

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Although nanozymes with intrinsic enzyme-like characteristics have aroused great interest in the biosensing field, the challenge is to keep high enzyme-like activity of the nanozyme after the modification of biomolecules onto nanozymes. Herein, a functional zonation strategy of a heterodimer nanozyme was proposed to tackle the challenge and further construct a multiple chemiluminescence (CL) imaging immunoassay. Here Fe 3 O 4 -Au as a heterodimer nanozyme model was divided into two zones, in which Fe 3 O 4 nanoparticles (NPs) were regarded as a nanozyme zone and AuNPs were defined as an antibody immobilization zone. A signal amplification probe (Fe 3 O 4 -Au-Ab 2 ) was prepared by modifying the secondary antibody (Ab 2 ) on AuNPs of the Fe 3 O 4 -Au heterodimer owing to the Au−S bond. The exposed Fe 3 O 4 of the Fe 3 O 4 -Au-Ab 2 probe shows very high peroxidase-like activity and can efficiently catalyze H 2 O 2 -luminol to produce strong CL imaging signals for multiple antigens detection. Using chicken interleukin-4 (ChIL-4) and chicken gamma interferon (ChIFN-γ) as models, the proposed CL imaging immunoassay shows wide linear ranges (0.005−0.10 ng/mL for both ChIL-4 and ChIFN-γ) and low detection limits (0.58 pg/mL for ChIL-4, 0.47 pg/mL for ChIFN-γ) with the characteristics of high sensitivity, high specificity, and good stability. This work provides a promising functional zonation concept for nanozymes to construct new types of nanozyme probes for immunoassay of multiple biomolecules.

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Section: Resultsmentioning

confidence: 79%

Functional Zonation Strategy of Heterodimer Nanozyme for Multiple Chemiluminescence Imaging Immunoassay

Shi,

Peng,

Zhu

et al. 2023

Anal. Chem.

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“…Therefore, the AuNPs are widely used in the field of biosensors. For instance, AuNPs had been used as DNA sensors for biomarkers, 10 and as functionalized sensors for small molecule substances. 11,12 Currently, mesoporous materials have been intensely pursued in chemical biology research because of their inherent characteristics, such as the desirable pore sizes (2 -50 nm), which are similar to the size of a number of drugs, protease, and DNA.…”

Section: Introductionmentioning

confidence: 99%

Based on Gold Nanoparticles-l-Tyr-Amino Functionalized Mesoporous Materials-Polyphenol Oxidase Modified Biosensor for the Detection of Resorcinol

et al. 2020

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Nowadays, resorcinol (RC) has been widely applied in the chemical and pharmaceutical industries. However, the electrochemical detection technique of RC still features some significant drawbacks, for instance, a low sensitivity. Hence, in the present work, a glass carbon electrode was developed for the electrochemical detection of RC with good specificity and stability, through modifying the glass carbon electrode (GCE) by polyphenol oxidase (PPO), an NH2-SBA-15 mesoporous material (NH2-SBA-15), L-tyrosine (L-Tyr) and gold nano-particles (AuNPs). After being successively modified by AuNPs, L-Tyr, NH2-SBA-15 and PPO, the constructed PPO/NH2-SBA-15/L-Tyr/AuNPs/GCE was used to discriminate RC from ions and other common micromolecules, which showed a fairly good specificity and stability. The proposed electrochemical detection method features a linear range of from 0.5 to 21.0 μM with a LOD down to 0.15 μM, revealing a better sensitivity than the existing methods. It is worth mentioning that the proposed PPO/NH2-SBA-15/L-Tyr/ AuNPs/GCE has been successfully used as an electrochemical probe for the RC assay in domestic sewage.

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“…53 Compared with APTES, pTN can be easily modified on various conductive matrixes 54,55 directly without any special functional group which would expand the application of pTN based immobilization methodology. 56,57 The performances of this type of biosensor in determining catechol were investigated. Meanwhile, the morphology of modified GCE surface, the parameters such as pH, enzyme concentration, and analytical performances were discussed and optimized in this paper.…”

Section: Introductionmentioning

confidence: 99%

Tyrosinase Modified Poly(thionine) Electrodeposited Glassy Carbon Electrode for Amperometric Determination of Catechol

Wang

Zhang

et al. 2017

Electrochemistry

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A stepwise strategy of mediator-free amperometric biosensor for the detection of catechol was developed based on the covalent bonding of tyrosinase (TYR) onto thionine (TN)-electrodeposited glassy carbon (GC) surface via glutaraldehyde (GA). Prior to the TYR-immobilization, poly(thionine) was prepared on a GC electrode surface by an electrooxidative polymerization of thionine. The TYR/GA/pTN modified electrode was evaluated by SEM and EIS measurements. The terminal amino groups (-NH 2 ) which electrodeposited on the GC surface were cross-linked with protein lysine group (or cysteine group) by GA. The resulting TYR/GA/pTN-immobilized GCE was utilized as a working electrode unit of a catechol-detect biosensor. Catechol was used as model analyte for the evaluation of catecholase activity, and the signal based on the electro-reduction of the enzymatically produced o-quinone species were monitored at −0.05 V vs. Ag/AgCl. The resulting TYR/GA/pTN/GCE biosensor exhibited rapid and sensitive response to catechol (100% response time: ≈5 s, sensitivity: 5.04 µA/mM, detection limit: 6.0 µM. The TYR/GA/ pTN/GCE retained 71% of original activity for catechol oxidation after 1 month storage.

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A novel impedimetric immunosensor for the detection of chicken interferon-gamma based on a polythionine and gold nanoparticle modified glassy carbon electrode

Cited by 9 publications

References 53 publications

Functional Zonation Strategy of Heterodimer Nanozyme for Multiple Chemiluminescence Imaging Immunoassay

Functional Zonation Strategy of Heterodimer Nanozyme for Multiple Chemiluminescence Imaging Immunoassay

Based on Gold Nanoparticles-l-Tyr-Amino Functionalized Mesoporous Materials-Polyphenol Oxidase Modified Biosensor for the Detection of Resorcinol

Tyrosinase Modified Poly(thionine) Electrodeposited Glassy Carbon Electrode for Amperometric Determination of Catechol

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