A novel immobilization fluorescence capillary analysis method and its applications

Abstract

Fluorescence capillary analysis (FCA) realizes trace-level analysis of micro-volume samples; it is easy to operate, extremely low in analytical cost and can significantly lessen environmental pollution from analytical chemistry waste.

On-line monitoring of a recirculating-flow fluorescent capillary system for exploring the interaction mechanism of carbon dots/metal ions

“…To address these issues, we designed an RF-FCA system with online fluorescence monitoring based on recirculating-flow (RF) 33 and fluorescence capillary analysis (FCA). 34 The RF-FCA system is a fluorescence kinetics method for online monitoring of the interaction processes between metal ions and CDs. To exclude the interference of unbound metal ions and their hydrolysates and prevent the aggregation-induced fluorescence quenching of CDs, the separation and purification process of CDs/metal complexes was monitored online by integrating the immobilized CDs into the RF-FCA.…”

Sensitive and selective detection of p-nitroaniline with the assistance of a fluorescence capillary imprinted sensor

“…Fluorescence capillary analysis (FCA) uses capillary with microcavity structure instead of traditional fluorescence detection cells. 13 Reagent usage can be reduced to 1/400 of conventional fluorescence analysis, greatly reducing biological and chemical waste. At present, the method has been successfully applied to the determination of glucose 14 and bile acid 15 because of its advantages of simple operation, small amounts of sample and reagent (≤18 μL), and reusability.…”