A novelabi5allele reveals the importance of the conserved Ala in the C3 domain for regulation of downstream genes and salt tolerance during germination inArabidopsis

Tezuka, Kenji; Taji, Teruaki; Hayashi, Takahisa; Sakata, Yoichi

doi:10.4161/psb.23455

Cited by 22 publications

(22 citation statements)

References 64 publications

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“…Since ABI3 is known to interact with ABI5 and other group A bZIP proteins (Hobo et al, 1999;Nakamura et al, 2001;BrocardGifford et al, 2003;Finkelstein et al, 2005;Marella et al, 2006;Park et al, 2011;Tezuka et al, 2013), our results together with the previous findings suggest that ABI3, ABI5, and DELLAs interact with each other on the SOM promoter to activate SOM mRNA expression at high temperature.…”

Section: Abi3 Abi5 and Della Proteins Bind To Each Othersupporting

confidence: 63%

“…Thus, seed germination is strongly inhibited at high temperature. bZIPs, including ABI5 and PIL5 (Hobo et al, 1999;Nakamura et al, 2001;Brocard-Gifford et al, 2003;Finkelstein et al, 2005;Park et al, 2011;Tezuka et al, 2013), their enrichment could be facilitated by the formation of a protein complex between ABI3 and other transcription factors on the SOM promoter. Such proteinprotein interactions could strengthen an otherwise weak targeting of transcription factors to a promoter.…”

Section: Abi3 Directly Activates Som Expression At High Temperaturementioning

confidence: 99%

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ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression ofSOMNUSand Other High-Temperature-Inducible Genes in Imbibed Seeds inArabidopsis

Lim

Park²,

Lee³

et al. 2013

The Plant Cell

210

174

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Seeds monitor the environment to germinate at the proper time, but different species respond differently to environmental conditions, particularly light and temperature. In Arabidopsis thaliana, light promotes germination but high temperature suppresses germination. We previously reported that light promotes germination by repressing SOMNUS (SOM). Here, we examined whether high temperature also regulates germination through SOM and found that high temperature activates SOM expression. Consistent with this, som mutants germinated more frequently than the wild type at high temperature. The induction of SOM mRNA at high temperature required abscisic acid (ABA) and gibberellic acid biosynthesis, and ABA-INSENSITIVE3 (ABI3), ABI5, and DELLAs positively regulated SOM expression. Chromatin immunoprecipitation assays indicated that ABI3, ABI5, and DELLAs all target the SOM promoter. At the protein level, ABI3, ABI5, and DELLAs all interact with each other, suggesting that they form a complex on the SOM promoter to activate SOM expression at high temperature. We found that high-temperature-inducible genes frequently have RY motifs and ABA-responsive elements in their promoters, some of which are targeted by ABI3, ABI5, and DELLAs in vivo. Taken together, our data indicate that ABI3, ABI5, and DELLAs mediate high-temperature signaling to activate the expression of SOM and other high-temperature-inducible genes, thereby inhibiting seed germination.

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Section: Abi3 Abi5 and Della Proteins Bind To Each Othersupporting

confidence: 63%

Section: Abi3 Directly Activates Som Expression At High Temperaturementioning

confidence: 99%

ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression ofSOMNUSand Other High-Temperature-Inducible Genes in Imbibed Seeds inArabidopsis

Lim

Park²,

Lee³

et al. 2013

The Plant Cell

210

174

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“…We transiently expressed the constructs in Arabidopsis mesophyll protoplasts and monitored transcriptional activity via LUCIFERASE (LUC) expression driven by the EM6 promoter (Fig. 8C), a well-defined ABI5-responsive promoter (Carles et al, 2002;Tezuka et al, 2013). As was observed in our yeast one-hybrid assays, the expression of wild-type ABI5 induced more than a 3-fold increase in LUC activity relative to the vector control (Fig.…”

Section: 2002;mentioning

confidence: 66%

SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5

et al. 2015

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Abscisic acid (ABA) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINASE5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, ABSCISIC ACID-INSENSITIVE5 (ABI5). We found that pks5-3 and pks5-4, two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast (Saccharomyces cerevisiae), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5. PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA-responsive gene expression. This phosphorylation was induced by ABA in vivo and transactivated ABI5. Expression of ABI5, in which Ser-42 was mutated to alanine, could not fully rescue the ABA-insensitive phenotypes of the abi5-8 and pks5-4abi5-8 mutants. In contrast, mutating Ser-42 to aspartate rescued the ABA insensitivity of these mutants. These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the ABA regulation of seed germination and gene expression in Arabidopsis (Arabidopsis thaliana).

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“…Previous studies showed that ABI3 interacts with ABI5 and regulates an overlapping set of physiological events as a regulatory complex (Nakamura et al ., ; Tezuka et al ., ). Thus, we further investigated the relationship between the ABI5–BES1 and ABI3–ABI5 interactions.…”

Section: Resultsmentioning

confidence: 97%

BES1 hinders ABSCISIC ACID INSENSITIVE5 and promotes seed germination in Arabidopsis

et al. 2018

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Proper regulation of seed germination is essential for the successful propagation of a plant. The transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5) of the abscisic acid (ABA) signaling pathway plays a central role in the inhibition of seed germination. ABI5 is precisely regulated by the core ABA signaling components and multiple other factors. However, the complex regulatory network of ABI5 remains largely unknown. In this study, we determined the biochemical interaction between ABI5 and the BRINSENSITIVE1 (BRI1)-EMS-SUPPRESSOR1 (BES1) transcription factor of the brassinosteroid (BR) signaling pathway, as well as the function of BES1 regulating ABI5 during seed germination in Arabidopsis. BES1 directly interacts with ABI5 both in vitro and in vivo. The bZIP domain of ABI5, which is responsible for DNA binding, is critical for ABI5 binding to BES1. The interaction of BES1 with ABI5 significantly suppressed the binding of ABI5 to the promoter regions of downstream genes, which resulted in their reduced expression and consequently facilitated seed germination. This study shed new light on the coordination of multiple signaling pathways during seed germination. In particular, BES1 directly binds to ABI5, which interferes with its transcriptional activity and suppresses ABA signaling output.

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A novelabi5allele reveals the importance of the conserved Ala in the C3 domain for regulation of downstream genes and salt tolerance during germination inArabidopsis

Cited by 22 publications

References 64 publications

ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression ofSOMNUSand Other High-Temperature-Inducible Genes in Imbibed Seeds inArabidopsis

ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression ofSOMNUSand Other High-Temperature-Inducible Genes in Imbibed Seeds inArabidopsis

SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5

BES1 hinders ABSCISIC ACID INSENSITIVE5 and promotes seed germination in Arabidopsis

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