A Novel HPLC Method Validation Based on Analytical Techniques of Metoclopramide Benzamide Derivative (Metoclopramide Base) and its Determination from Solid Dispersion by Solvent Evaporation Method

Abstract

The crucial endeavor in this method is focused on the study analysing recovery and column efficiency (theoretical plate) after extraction of metoclopramide from PVP K30-Metoclopramide (3:1 w/w) solid dispersion (SD). The proposed work also presents a reversed-phase high performance liquid chromatography method for the detection of very small amount of metoclopramide base followed by different analytical techniques. In this context, it may be noted that the values of limit of detection (LOD) and limit of quantitation (LOQ) for the metoclopramide base as obtained are, 0.052 µg/mL and 0.159 µg/mL respectively. The linearity is performed in the range of 2-20 µg/ mL; performance of column remained satisfactory throughout the analysis, N = 4927-2434. The percentage recovery of pure and extracted metoclopramide ranges from 103-105% and 86-120% approximately. The results collectively demonstrate that the proposed method is selective. Clear isolation of metoclopramide from SD is achieved without any solid state interference.

Using of Oxidative - Coupling Reaction in Spectrophotometric Determination of Metoclopramide Hydrochloride in Pharmaceutical Preparations

“…There are many different techniques that have been used for the determination of metoclopramide by various methods, including spectrophotometric methods [6,15], HPLC [16][17][18][19][20][21], potentiometric [22], Densitometric and UPLC methods [23], fluorometric [24], Flow Injection Chemiluminescence Technique [25], voltammetric [26], electrochemiluminescence [27],Capillary electrophoresis [28],LC-MS/MS [29], Atomic Absorbance [30] and molar refraction and polarizability method [31]. The main aim of the current work is to suggest an accurate and sensitive visible spectrophotometric method for the assay of METH as pure and in dosage form via oxidative…”